Provides an easily accessible reference volume for analysis of chromatin structure and function incorporating the latest techniques
Includes detailed, step by step guidelines for the analysis of chromatin structure and its role in tranillegalscription, replication and other biological processes
Details elaborate protocols for the detection and characterization of histone modifications including acetylation, methylation and ubiquitination
Describes protocols for techniques ranging from immunohistochemical detection of chromatin modifications to genome-wide analysis of chromatin associated proteins
Acts as a comprehensive guide to the most up to date techniques dealing with all aspects of chromatin biology
Presents experimental details in an easy to follow modular format typical of the MiMB series
Provides an easily accessible reference volume for analysis of chromatin structure and function incorporating the latest techniques
Includes detailed, step by step guidelines for the analysis of chromatin structure and its role in tranillegalscription, replication and other biological processes
Details elaborate protocols for the detection and characterization of histone modifications including acetylation, methylation and ubiquitination
Describes protocols for techniques ranging from immunohistochemical detection of chromatin modifications to genome-wide analysis of chromatin associated proteins
Acts as a comprehensive guide to the most up to date techniques dealing with all aspects of chromatin biology
Presents experimental details in an easy to follow modular format typical of the MiMB series
Includes supplementary material: sn.pub/extras
Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular Biology™ series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.
1. Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila EmbryosEdgar Bonte and Peter B. Becker2. Analysis of Reconstituted Chromatin Using a Solid-Phase ApproachRaphael Sandaltzopoulos and Peter B. Becker3. In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Characteristics of Selected ProteinsSherry L. Winter, Philip Wong, and Mark G. Alexandrow4. DNA Methyltransferase Probing of Chromatin Structure within Populations and on Single MoleculesCarolina Pardo, Scott A. Hoose, Santhi Pondugula, and Michael P. Kladde5. Visualization of the Expression of HMGN Nucleosomal Binding Proteins in the Developing Mouse Embryo and in Adult Mouse TissuesTakashi Furusawa and Michael Bustin 6. Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase ChromatinEisuke Gotoh 7. Analysis of DNA Topology in Yeast ChromatinRandall H. Morse8. Preparation and Analysis of Uniquely Positioned MononucleosomesDaria A. Gaykalova, Olga I. Kulaeva, Vladimir A. Bondarenko, and Vasily M. Studitsky9. Monitoring DNA Breaks in Optically Highlighted Chromatin in Living Cells by Laser Scanning Confocal MicroscopyMichael J. Kruhlak, Arkady Celeste, and André Nussenzweig10. Methods to Study Tranillegalscription-Coupled Repair in ChromatinHélène Gaillard, Ralf Erik Wellinger, and Andrés Aguilera11. Cytometric Analysis of DNA Damage: Phosphorylation of Histone H2AX as a Marker of DNA Double-Strand Breaks (DSBs)Toshiki Tanaka, Dorota Halicka, Frank Traganos, and Zbigniew Darzynkiewicz12. Detection of DNA Damage Induced by Topoisomerase II Inhibitors, Gamma Radiation and Crosslinking Agents Using the Comet AssayLori A.Hazlehurst13. Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase ChromosomesMelanie Carless14. In Vitro Replication Assay with Mammalian Cell ExtractsWasia Rizwani and Srikumar P. Chellappan15. Investigation of Genomic Methylation Status Using Methylation Specific and Bisulfite Sequencing Polymerase Chain ReactionMelanie Carless16. Biochemical Analysis of Arginine Methylation in TranillegalscriptionMarc Tini, Hina Naeem, and Joseph Torchia17. In Vitro Histone Demethylase AssaysKenji Kokura and Jia Fang18. Histone Chaperone as Coactivator of Chromatin Tranillegalscription: Role of Acetylation Shrikanth S. Gadad, Jayasha Shandilya, Venkatesh Swaminathan, and Tapas K. Kundu19. Histone Deacetylase Activity AssayZhigang Yuan, Natalie Rezai-Zadeh, Xiaohong Zhang, and Edward Seto20. In vitro and in vivo Assays for Studying Histone Ubiquitination and DeubiquitinationLing Zhai, Heui-Yun Joo, and Hengbin Wang21. Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by ImmunostainingJaya Padmanabhan22. Chromatin Immunoprecipitation Assays: Analyzing Tranillegalscription Factor Binding and Histone Modifications in vivoSmitha Pillai, Piyali Dasgupta, and Srikumar P. Chellappan23. ChIP on Chip Assays: Genomewide Analysis of Tranillegalscription Factor Binding and Histone ModificationsSmitha Pillai and Srikumar P. Chellappan24. ChIP-on-Chip Analysis Methods for Affymetrix Tiling ArraysSean J. Yoder and Steven A. Enkemann25. Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere LengthPartha P. Banerjee and Shankar Jagadeesh26. Detecting ATM Dependent Chromatin Modification in DNA Damage a
Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular Biology™ series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.
From the reviews of the second edition:"This book contains a collection of detailed and useful procedures concerning chromatin analysis. It includes methods for the isolation and assembly of nucleosomes, the analysis of basic chromatin structure, the covalent modification of histones and the analysis of chromatin function. ... In summary, this is a very useful text, with lots of strengths ... .” (Ray Waters, Human Genetics, Vol. 126, 2009)
Comprehensive and up-to-date, this revised second edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.
Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular Biology(TM) series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.
Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos.- Analysis of Reconstituted Chromatin Using a Solid-Phase Approach.- In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Characteristics of Selected Proteins.- DNA Methyltransferase Probing of Chromatin Structure Within Populations and on Single Molecules.- Visualization of the Expression of HMGN Nucleosomal Binding Proteins in the Developing Mouse Embryo and in Adult Mouse Tissues.- Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin.- Analysis of DNA Topology in Yeast Chromatin.- Preparation and Analysis of Uniquely Positioned Mononucleosomes.- Monitoring DNA Breaks in Optically Highlighted Chromatin in Living Cells by Laser Scanning Confocal Microscopy.- Methods to Study Tranillegalscription-Coupled Repair in Chromatin.- Cytometric Analysis of DNA Damage: Phosphorylation of Histone H2AX as a Marker of DNA Double-Strand Breaks (DSBs).- Detection of DNA Damage Induced by Topoisomerase II Inhibitors, Gamma Radiation and Crosslinking Agents Using the Comet Assay.- Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes.- In Vitro Replication Assay with Mammalian Cell Extracts.- Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Polymerase Chain Reaction.- Biochemical Analysis of Arginine Methylation in Tranillegalscription.- In Vitro Histone Demethylase Assays.- Histone Chaperone as Coactivator of Chromatin Tranillegalscription: Role of Acetylation.- Histone Deacetylase Activity Assay.- In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination.- Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining.- Chromatin Immunoprecipitation Assays: Analyzing Tranillegalscription Factor Binding and Histone Modifications In Vivo.- ChIP on Chip Assays: Genome-Wide Analysis of Tranillegalscription Factor Binding and Histone Modifications.- ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays.- Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length.- Detecting ATM-Dependent Chromatin Modification in DNA Damage and Heat Shock Response.
From the reviews of the second edition: "This book contains a collection of detailed and useful procedures concerning chromatin analysis. It includes methods for the isolation and assembly of nucleosomes, the analysis of basic chromatin structure, the covalent modification of histones and the analysis of chromatin function. ... In summary, this is a very useful text, with lots of strengths ... ." (Ray Waters, Human Genetics, Vol. 126, 2009)
Inhaltsverzeichnis
Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos.- Analysis of Reconstituted Chromatin Using a Solid-Phase Approach.- In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Characteristics of Selected Proteins.- DNA Methyltransferase Probing of Chromatin Structure Within Populations and on Single Molecules.- Visualization of the Expression of HMGN Nucleosomal Binding Proteins in the Developing Mouse Embryo and in Adult Mouse Tissues.- Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin.- Analysis of DNA Topology in Yeast Chromatin.- Preparation and Analysis of Uniquely Positioned Mononucleosomes.- Monitoring DNA Breaks in Optically Highlighted Chromatin in Living Cells by Laser Scanning Confocal Microscopy.- Methods to Study Tranillegalscription-Coupled Repair in Chromatin.- Cytometric Analysis of DNA Damage: Phosphorylation of Histone H2AX as a Marker of DNA Double-Strand Breaks (DSBs).- Detection of DNA Damage Induced by Topoisomerase II Inhibitors, Gamma Radiation and Crosslinking Agents Using the Comet Assay.- Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes.- In Vitro Replication Assay with Mammalian Cell Extracts.- Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Polymerase Chain Reaction.- Biochemical Analysis of Arginine Methylation in Tranillegalscription.- In Vitro Histone Demethylase Assays.- Histone Chaperone as Coactivator of Chromatin Tranillegalscription: Role of Acetylation.- Histone Deacetylase Activity Assay.- In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination.- Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining.- Chromatin Immunoprecipitation Assays: Analyzing Tranillegalscription Factor Binding and Histone Modifications In Vivo.- ChIP on Chip Assays: Genome-Wide Analysis of Tranillegalscription Factor Binding and Histone Modifications.- ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays.- Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length.- Detecting ATM-Dependent Chromatin Modification in DNA Damage and Heat Shock Response.
Klappentext
Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular Biology¿ series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.nnComprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.
Provides an easily accessible reference volume for analysis of chromatin structure and function incorporating the latest techniques
Includes detailed, step by step guidelines for the analysis of chromatin structure and its role in tranillegalscription, replication and other biological processes
Details elaborate protocols for the detection and characterization of histone modifications including acetylation, methylation and ubiquitination
Describes protocols for techniques ranging from immunohistochemical detection of chromatin modifications to genome-wide analysis of chromatin associated proteins
Acts as a comprehensive guide to the most up to date techniques dealing with all aspects of chromatin biology
Presents experimental details in an easy to follow modular format typical of the MiMB series
Includes supplementary material: sn.pub/extras
