Brings recent developments to the forefront in a timely manner
Presents scientific advances in stem cell methods for a wider use by novice and expert scientists
The scope for improving health care using stem cell therapies is thrilling, but has considerable technical challenges and methodological constraints that need to be addressed. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, this book presents scientific advances in stem cell methods for a wider use by novice and expert scientists, through the series of Methods in Molecular Biology.
|Stem cells and regenerative medicine is a fast emerging field with rapid strides of progress and focus on human health. Successful utilization of stem cells in regenerative medicine depends on two important features. One, stem cells can grow and divide indefinitely, and two, stem cells can differentiate into specialized cell types that make up tissues such as pancreas, heart, liver, blood, and others. Utilizing the property of differentiation, stem cells can be engineered to produce cell types to replace wornout cells and to regenerate damaged tissue. The scope for improving healthcare using stem cell therapies is thrilling, but considerable technical challenges and methodological constraints need to be addressed. There is a great need for isolation and propagation of "transplant-ready” or "clinical-grade” stem cells and screening methods, well before they are used in therapies. Recent scientific advances have contributed enormously to the development of new methods pertaining to both embryonic and adult stem cell isolation, characterization, expansion in undifferentiated state, customized differentiation, efficient stem cell transduction for gene therapy, quick monitoring of rejection, through polymerase chain reaction approaches following transplantation, and more. The scientific progress that makes stem cell biology so exciting also threatens to suffocate researchers with an avalanche of information. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, the book Stem Cell Assays aims to present scientific advances in stem cell methods for a wider use by novice and expert scientists, through the Methods in Molecular Biology series.
Book Title: Stem Cell Assays Publisher: Humana PressEditor : Mohan C. Vemuri SeriesEditor : John WalkerSeries : Methods in Molecular Biology Date ofRelease: Late 2006 / Early 2007ContentsPrefaceContributorsGuide to the companion CD1 Derivation of Human Embryonic Stem Cells in xeno-free conditionsMohan C. Vemuri, Tim Schimmel, Pere Colls, Santiago Munne and JacquesCohen2 Feeder Layer Free Culture System For Human Embryonic Stem CellsMichal Amit3 Digital Imaging of Stem Cells by Electron MicroscopyHenry Sathananthan and Stefania Nottola4 A Controlled Cooling Protocol For Cryopreservation Of Human AndNon-Human Primate Embryonic Stem CellsCarol B. Ware and Szczepan Baran5 Cell Surface Markers In Human Embryonic Stem CellsRaj R. Rao, Alison Venable Johnson and Steven L. Stice6 Generation Of Monoclonal Antibody Library Against Human EmbryonicStem CellsMicha Drukker, Christina Muscat, Irving L. Weissman7 Analytical Methods for Cancer Stem Cells - Review chapterVinagolu K. Rajasekhar8 Micro RNA Profiling: An Easy and Rapid Method to Screen andCharacterize Stem Cell PopulationsUma Lakshmipathy, Bradley Love, Christopher Adams, BhaskarThyagarajan, andJonathan D. Chesnut9 Gene Transfer Via Nucleofection into Adult And Embryonic Stem CellsUma Lakshmipathy, Shannon Buckley and Catherine Verfaillie10 RNAi-Knockdown Of Tranillegalscription Factor Pu.1 In The DifferentiationOf Mouse EmbryonicStem CellsGang-Ming Zou, Meredith A. Thompson and Mervin C. Yoder11 StemBase - A Resource for the Analysis of Stem Cell Gene ExpressionData - Review chapterChristopher J. Porter, Gareth A. Palidwor, Reatha Sandie, Paul M.Krzyzanowski, Enrique M. Muro, Carolina Perez-Iratxeta, Miguel A.Andrade-Navarro12 Isolation Of Stem Cells From Human Umbilical Cord BloodNishant P. Reddy, Mohan C. Vemuri and Reddanna Pallu13 Ex Vivo Expansion Of Hematopoietic Stem Cells From Human Cord BloodIn Serum-Free ConditionsChao-Ling Yao and Shiaw-Min Hwang14 Hematopoietic Colony Forming Cell AssaysCarla Pereira, Emer Clarke and Jackie Damen15 Assays For Alloreactive Responses By PCRPatrick Stordeur16 Immune Properties of Mesenchymal Stem Cells Review chapterPanagiota A. Sotiropoulou and Michael Papamichail17 Clinical Grade Expansion of Human Bone Marrow Mesenchymal Stem CellsPanagiota A. Sotiropoulou, Sonia A. Perez and Michael Papamichail18 Adenoviral Transduction of Mesenchymal Stem CellsPablo Bosch and Steven L. Stice19 Directed Differentiation of Human Embryonic Stem Cells to DendriticCellsMaxim A. Vodyanik and Igor I. Slukvin20 Insulin Producing Cells From Embryonic Stem Cells: ExperimentalConsiderationsEnrique Roche, Roberto Ensenat-Waser, Nestor Vicente-Sala, AlfredoSantana,Martin Zenke and Juan Antonio Rei21 Efficient Generation of Dopamine Neurons from Human Embryonic Stem(hES) CellsChang-Hwan Park and Sang-Hun Lee22 Isolation Of Oligodendroglial Cells From Cultured NeuralStem/ProgenitorsFrank Zeigler and Stephen G. Hall23 Differentiation of Human Embryonic Stem Cells towards theChondrogenic LineageWei Seong Toh, Zheng Yang Boon Chin Heng and Tong Cao24 Cartilage Tissue Engineering: Directed differentiation of embryonicstem cells in Three-Dimensional Hydrogel CultureNathaniel S. Hwang, Shyni Varghese and Jennifer Elisseeff
With such benefits as producing cell types to replace worn-out cells and regenerating damaged tissue, stem cells and regenerative medicine is a rapidly emerging field focusing on improving human health. In Stem Cell Assays, an expert team of hands-on researchers contribute step-by-step laboratory protocols aimed at clearly organizing the avalanche of information coming from the stem cell biology field. Following the successful and practical Methods in Molecular Biology™ series format, each chapter provides concise methods utilizing the most recent developments in embryonic and adult stem cell isolation, characterization, customized differentiation and many more areas, a complete list of the necessary materials, and the valuable Notes section for tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Stem Cell Assays brings the newest developments to the forefront in an invaluable reference volume for both novice and expert scientists alike.
From the reviews:
"The present volume `Stem Cell Assays´ ... include very detailed experimental sections, which are essentially raw lab protocols. ... All protocols are explained in full detail ... and should be very useful for researchers novel to this field. In addition, for more experienced scientists it may be very useful to compare their own protocols with those provided in this volume ... . this book will be of great value for experimental scientists that wish to introduce novel technology into their labs." (Gerald de Haan, Molecular Biotechnology, May, 2008)
Stem cells and regenerative medicine is a fast emerging field with rapid strides of progress and focus on human health. Successful utilization of stem cells in regenerative medicine depends on two important features. One, stem cells can grow and divide indefinitely, and two, stem cells can differentiate into specialized cell types that make up tissues such as pancreas, heart, liver, blood, and others. Utilizing the property of differentiation, stem cells can be engineered to produce cell types to replace wornout cells and to regenerate damaged tissue. The scope for improving healthcare using stem cell therapies is thrilling, but considerable technical challenges and methodological constraints need to be addressed. There is a great need for isolation and propagation of "transplant-ready" or "clinical-grade" stem cells and screening methods, well before they are used in therapies. Recent scientific advances have contributed enormously to the development of new methods pertaining to both embryonic and adult stem cell isolation, characterization, expansion in undifferentiated state, customized differentiation, efficient stem cell transduction for gene therapy, quick monitoring of rejection, through polymerase chain reaction approaches following transplantation, and more. The scientific progress that makes stem cell biology so exciting also threatens to suffocate researchers with an avalanche of information. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, the book Stem Cell Assays aims to present scientific advances in stem cell methods for a wider use by novice and expert scientists, through the Methods in Molecular Biology series.
Preface.- Contributors.- Derivation of Human Embryonic Stem Cells in Xeno-Free ConditionsMohan C. Vemuri, Tim Schimmel, Pere Colls, Santiago Munne, and Jacques Cohen.- Feeder-Layer Free Culture System for Human Embryonic Stem CellsMichal Amit.- Digital Imaging of Stem Cells by Electron MicroscopyA. Henry Sathananthan and Stefania A. Nottola.- A Controlled-Cooling Protocol for Cryopreservation of Human and Non-Human Primate Embryonic Stem CellsCarol B. Ware and Szczepan W. Baran.- Cell Surface Markers in Human Embryonic Stem CellsRaj R. Rao, Alison Venable Johnson, and Steven L. Stice.- Generation of a Monoclonal Antibody Library Against Human Embryonic Stem CellsMicha Drukker, Christina Muscat, and Irving L. Weissman.- Analytical Methods for Cancer Stem CellsVinagolu K. Rajasekhar.- Micro RNA Profiling: An Easy and Rapid Method to Screen and Characterize Stem Cell PopulationsUma Lakshmipathy, Brad Love, Christopher Adams, Bhaskar Thyagarajan, and Jonathan D. Chesnut.- Gene Transfer Via Nucleofection Into Adult and Embryonic Stem CellsUma Lakshmipathy, Shannon Buckley, and Catherine Verfaillie.- RNAi Knockdown of Tranillegalscription Factor Pu.1 in the Differentiation of Mouse Embryonic Stem CellsGang-Ming Zou, Meredith A. Thompson, and Mervin C. Yoder.- StemBase: A Resource for the Analysis of Stem Cell Gene Expression DataChristopher J. Porter, Gareth A. Palidwor, Reatha Sandie, Paul M. Krzyzanowski, Enrique M. Muro, Carolina Perez-Iratxeta, and Miguel A. Andrade-Navarro.- Isolation of Stem Cells from Human Umbilical Cord BloodNishanth P. Reddy, Mohan C. Vemuri, and Reddanna Pallu.- Ex Vivo Expansion of Hematopoietic Stem Cells from Human Cord Blood in Serum-Free ConditionsChao-Ling Yao and Shiaw-Min Hwang.- Hematopoietic Colony-Forming Cell AssaysCarla Pereira, Emer Clarke,and Jackie Damen.- Assays for Alloreactive Responses by PCRPatrick Stordeur.- Immune Properties of Mesenchymal Stem CellsPanagiota A. Sotiropoulou and Michael Papamichail.- Clinical Grade Expansion of Human Bone Marrow Mesenchymal Stem CellsPanagiota A. Sotiropoulou, Sonia A. Perez, and Michael Papamichail.- Adenoviral Transduction of Mesenchymal Stem CellsPablo Bosch and Steven L. Stice.- Directed Differentiation of Human Embryonic Stem Cells to Dendritic CellsMaxim A. Vodyanik and Igor I. Slukvin.- Insulin-Producing Cells from Embryonic Stem Cells Experimental ConsiderationsEnrique Roche, Roberto Ensenat-Waser, Nestor Vicente-Salar, Alfredo Santana, Martin Zenke, and Juan Antonio Reig.- Efficient Generation of Dopamine Neurons from Human Embryonic Stem CellsChang-Hwan Park and Sang-Hun Lee.- Isolation of Oligodendroglial Cells from Cultured Neural Stem/ProgenitorsFrank Zeigler and Stephen G. Hall.- Differentiation of Human Embryonic Stem Cells Toward the Chondrogenic LineageWei Seong Toh, Zheng Yang, Boon Chin Heng, and Tong Cao.- Cartilage Tissue Engineering: Directed Differentiation of Embryonic Stem Cells in Three-Dimensional Hydrogel CultureNathaniel S. Hwang, Shyni Varghese, and Jennifer Elisseeff.
From the reviews:
"The present volume 'Stem Cell Assays' ... include very detailed experimental sections, which are essentially raw lab protocols. ... All protocols are explained in full detail ... and should be very useful for researchers novel to this field. In addition, for more experienced scientists it may be very useful to compare their own protocols with those provided in this volume ... . this book will be of great value for experimental scientists that wish to introduce novel technology into their labs." (Gerald de Haan, Molecular Biotechnology, May, 2008)
Inhaltsverzeichnis
Book Title: Stem Cell Assays nPublisher: Humana PressnEditor : Mohan C. Vemuri SeriesnEditor : John WalkernSeries : Methods in Molecular Biology Date ofnRelease: Late 2006 / Early 2007nnnContentsnnPrefacenContributorsnGuide to the companion CDnnn1 Derivation of Human Embryonic Stem Cells in xeno-free conditionsnMohan C. Vemuri, Tim Schimmel, Pere Colls, Santiago Munne and JacquesnCohennnn2 Feeder Layer Free Culture System For Human Embryonic Stem CellsnMichal Amitnnn3 Digital Imaging of Stem Cells by Electron MicroscopynHenry Sathananthan and Stefania Nottolannn4 A Controlled Cooling Protocol For Cryopreservation Of Human AndnNon-Human Primate Embryonic Stem CellsnCarol B. Ware and Szczepan Barannnn5 Cell Surface Markers In Human Embryonic Stem CellsnRaj R. Rao, Alison Venable Johnson and Steven L. Sticennn6 Generation Of Monoclonal Antibody Library Against Human EmbryonicnStem CellsnMicha Drukker, Christina Muscat, Irving L. Weissmannnn7 Analytical Methods for Cancer Stem Cells - Review chapternVinagolu K. Rajasekharnnn8 Micro RNA Profiling: An Easy and Rapid Method to Screen andnCharacterize Stem Cell PopulationsnUma Lakshmipathy, Bradley Love, Christopher Adams, BhaskarnThyagarajan, andnJonathan D. Chesnutnnn9 Gene Transfer Via Nucleofection into Adult And Embryonic Stem CellsnUma Lakshmipathy, Shannon Buckley and Catherine Verfailliennn10 RNAi-Knockdown Of Tranillegalscription Factor Pu.1 In The DifferentiationnOf Mouse EmbryonicnStem CellsnGang-Ming Zou, Meredith A. Thompson and Mervin C. Yodernnn11 StemBase - A Resource for the Analysis of Stem Cell Gene ExpressionnData - Review chapternChristopher J. Porter, Gareth A. Palidwor, Reatha Sandie, Paul M.nKrzyzanowski, Enrique M. Muro, Carolina Perez-Iratxeta, Miguel A.nAndrade-Navarronnn12 Isolation Of Stem Cells From Human Umbilical Cord BloodnNishant P. Reddy, Mohan C. Vemuri and Reddanna Pallunnn13 Ex Vivo Expansion Of Hematopoietic Stem Cells From Human Cord BloodnIn Serum-Free ConditionsnChao-Ling Yao and Shiaw-Min Hwangnnn14 Hematopoietic Colony Forming Cell AssaysnCarla Pereira, Emer Clarke and Jackie Damennnn15 Assays For Alloreactive Responses By PCRnPatrick Stordeurnnn16 Immune Properties of Mesenchymal Stem Cells Review chapternPanagiota A. Sotiropoulou and Michael Papamichailnnn17 Clinical Grade Expansion of Human Bone Marrow Mesenchymal Stem CellsnPanagiota A. Sotiropoulou, Sonia A. Perez and Michael Papamichailnnn18 Adenoviral Transduction of Mesenchymal Stem CellsnPablo Bosch and Steven L. Sticennn19 Directed Differentiation of Human Embryonic Stem Cells to DendriticnCellsnMaxim A. Vodyanik and Igor I. Slukvinnnn20 Insulin Producing Cells From Embryonic Stem Cells: ExperimentalnConsiderationsnEnrique Roche, Roberto Ensenat-Waser, Nestor Vicente-Sala, AlfredonSantana,nMartin Zenke and Juan Antonio Reinnnn21 Efficient Generation of Dopamine Neurons from Human Embryonic Stemn(hES) CellsnChang-Hwan Park and Sang-Hun Leennn22 Isolation Of Oligodendroglial Cells From Cultured NeuralnStem/ProgenitorsnnFrank Zeigler and Stephen G. Hallnnn23 Differentiation of Human Embryonic Stem Cells towards thenChondrogenic LineagenWei Seong Toh, Zheng Yang Boon Chin Heng and Tong Caonnn24 Cartilage Tissue Engineering: Directed differentiation of embryonicnstem cells in Three-Dimensional Hydrogel CulturenNathaniel S. Hwang, Shyni Varghese and Jennifer Elisseeff
Klappentext
The scope for improving health care using stem cell therapies is thrilling, but has considerable technical challenges and methodological constraints that need to be addressed. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, this book presents scientific advances in stem cell methods for a wider use by novice and expert scientists, through the series of Methods in Molecular Biology.
Brings recent developments to the forefront in a timely manner
Presents scientific advances in stem cell methods for a wider use by novice and expert scientists
