TABLE OF CONTENTSnnn1. Technology of Patch Clamp ElectrodesnJames L. Rae and Richard A. Levisnn2. Whole Cell Patch-Clamp RecordingsnHarald Sontheimer and Michelle L. Olsennn3. Single-Channel RecordingnDavid J.A. Wyllienn4. Combined Fluorometric and Electrophysiological RecordingsnHartmut Schmidt and Jens Eilersnn5. Combining Uncaging Techniques with Patch Clamp Recording and OpticalnPhysiologynDmitry V. Sarkisov and Samuel S.-H. Wangnn6. Visually-Guided Patch-Clamp Recordings in Brain SlicesnJames R. Moyer, Jr. and Thomas H. Brownnn7. In Vivo Patch-Clamp TechniquenHidemasa Furue, Toshihiko Katafuchi and Megumu Yoshimurann8. Techniques for Perforated Patch RecordingsnConstantine Sarantopoulosnn9. Fast-Drug ApplicationnManfred Heckmann and Stefan Hallermannnn10. Pipette Internal Perfusion: Methods and ApplicationsnSrinivas M. Tipparaju and Aruni Bhatnagarnn11. Loose-Patch Clamp MethodnHector G. Marrero and Jose R. Lemosnn12. Recording Currents from Channels and Transporters in MacropatchesnGuiying Cui, Matthew D. Fuller, Christopher H. Thompson, Zhi-RennZhang and Nael McCartynn13. Structure-Function Analyses of Single Cells by CombiningnPatch-Clamp Techniques with RT-PCRnGerald Seifert and Christian Steinhausernn14. Planar Patch ClampingnJan C. Behrends and Niels Fertignn15. Automated Glass Pipette-based Patch Clamp TechniquesnMichael Fejtl, Uwe Czubayko, Alexander Hummer, Tobias Krauternand Albrecht Lepple-Wienhues
Continuing the research of the best-selling first edition, this second edition collects three more years of research in the ever-expanding study of the cell membrane. It covers the latest developments in the "traditional" patch techniques. This authoritative second edition updates the standard techniques while introducing three brand new, cutting-edge technical advances in the field.
Thorough and timely, this edition is an invaluable resource.
Provides readily reproducible, step-by-step protocols that ensure experimental success
Describes methods combining patch-clamp with molecular biology and imaging
Includes new state-of-the-art techniques