Details how electrophysiology can be best utilized in drug discovery and development
Introduces applications of the dynamic clamp method
Includes an extensive theoretical treatise concerning single channel kinetic analysis
Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training, and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is organized by the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. Individual techniques are explored within the area that they are applied most often. Researchers will find this text to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies.
Table of contentsPart 1. Pharmacology1. Pharmacological analysis of recombinant NR1a/2A and NR1a/2B NMDAreceptors using whole-cell patch clamp method. Laszlo Fodor and JozsefNagy2. Memantine as an example of a fast, voltage-dependent, open channelN-methyl-D-aspartate (NMDA) receptor blocker. Chris G. Parsons and KateGilling3. Methods for evaluation of positive allosteric modulators ofglutamate AMPA receptors. Edward R. Siuda, Jennifer C. Quirk and EricS. Nisenbaum4. Automated voltage clamp technique. Andrea Ghetti, Antonio Guia andJia Xu5. Flip-the-Tip: automated patch clamping based on glass electrodes.Michael Fejtl, Uwe Czubayko, Alexander Hümmer, Tobias Krauter andAlbrecht Lepple-Wienhues6. The Roboocyte: Automated Electrophysiology Based on Xenopus Oocytes.Christine Leisgen, Mike Kuester and Christoph MethfesselPart 2. Physiology7. Infrared guided laser stimulation as a tool for elucidating thesynaptic site of expression of long-term synaptic plasticity. GerhardRammes, Mathias Eder, Walter Zieglgänsberger and Hans-Ulrich Dodt8. Single-cell RT-PCR, a Technique to Decipher the Electrical,Anatomical and Genetic Determinants of Neuronal Diversity. MariaToledo-Rodriguez and Henry Markram9. Mechanosensitive Ion Channels investigated simultaneously byScanning Probe Microscopy and Patch clamp. Matthias G. Langer10. Synaptic Connectivity in Engineered Neuronal Networks. PeterMolnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das and James J.Hickman11. Modeling of Action Potential Generation in NG108-15 Cells. PeterMolnar and James J. Hickman12. Whole-cell Voltage-clamp on Skeletal Muscle Fibers with theSilicone-Clamp Technique. Sandrine Pouvreau, Claude Collet, BrunoAllard and Vincent Jacquemond13. Determination of channel properties at the unitary level in adultmammalian isolated cardiomyocytes. RomainGuinamard14. Electrophysiological Properties of Embryonic Stem Cells DuringDifferentiation into Cardiomyocyte-like Cell Types. Antoni C.G. vanGinneken and Arnoud. C. Fijnvandraat15. Hybrid Neuronal Network Studies Under Dynamic Clamp. Alan D. DorvalII, Jonathan Bettencourt, Theoden I. Netoff and John A. White16. Cardiac channelopathies studied with the dynamic action potentialclamp technique. Géza Berecki, Jan G. Zegers, Ronald Wilders and AntoniC.G. van GinnekenPart 3. Biophysics17. Principles of Single-Channel Kinetic Analysis. Feng Qin *Theoretical chapter18. Use of Xenopus oocytes to measure ionic selectivity of pore-formingpeptides and ion channels. Thierry Cens and Pierre Charnet19. Estimation of quantal parameters with multiple-probabilityfluctuation analysis. Chiara Saviane and Robin Angus Silver
Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is divided into three sections that cover the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. The first section provides examples and step by step instructions on how to use whole-cell and single-channel patch clamp methods for testing drugs in industrial settings. The second section provides a wide selection of patch clamp applications in physiological studies. The last part focuses on the biophysical applications of the patch clamp method using single channel recordings or statistical analysis of whole-cell currents in order to obtain parameters that describe ion channel properties or transmitter release. Individual techniques are explored within the area that they are applied most often. Researchers will find Patch Clamp Methods and Protocols to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies.
From the reviews:
"This book will be helpful to researchers already working in the field of electrophysiology, but also those coming to this field from pharmacological, physiological, and biophysical areas." - Doody's Book Review Service
"'Patch-clamp: Methods and Protocols´ introduces some common patch-clamp applications. It is intended to assist scientists in identifying problems, which could be best addressed by this technique. The book provides the step-by-step procedures to perform the experiments that answer those questions. ... It will be a useful companion for moderately advanced users wishing to extend their skills." (Maria Drigelova, General Physiology and Biophysics, Vol. 27, April, 2008)
Pharmacology.- Pharmacological Analysis of Recombinant NR1a/2A and NR1a/2B NMDA Receptors Using the Whole-Cell Patch-Clamp Method.- Memantine as an Example of a Fast, Voltage-Dependent, Open Channel N-Methyl-d-Aspartate Receptor Blocker.- Methods for Evaluation of Positive Allosteric Modulators of Glutamate AMPA Receptors.- Automated Voltage-Clamp Technique.- Flip-the-Tip.- The Roboocyte.- Physiology.- Infrared-Guided Laser Stimulation as a Tool for Elucidating the Synaptic Site of Expression of Long-Term Synaptic Plasticity.- Single-Cell RT-PCR, a Technique to Decipher the Electrical, Anatomical, and Genetic Determinants of Neuronal Diversity.- Mechanosensitive Ion Channels Investigated Simultaneously by Scanning Probe Microscopy and Patch Clamp.- Synaptic Connectivity in Engineered Neuronal Networks.- Modeling of Action Potential Generation in NG108-15 Cells.- Whole-Cell Voltage Clamp on Skeletal Muscle Fibers With the Silicone-Clamp Technique.- Determination of Channel Properties at the Unitary Level in Adult Mammalian Isolated Cardiomyocytes.- Electrophysiological Properties of Embryonic Stem Cells During Differentiation Into Cardiomyocyte-Like Cell Types.- Hybrid Neuronal Network Studies Under Dynamic Clamp.- Cardiac Channelopathies Studied With the Dynamic Action Potential-Clamp Technique.- Biophysics.- Principles of Single-Channel Kinetic Analysis.- Use of Xenopus Oocytes to Measure Ionic Selectivity of Pore-Forming Peptides and Ion Channels.- Estimation of Quantal Parameters With Multiple-Probability Fluctuation Analysis.
From the reviews:
"This book will be helpful to researchers already working in the field of electrophysiology, but also those coming to this field from pharmacological, physiological, and biophysical areas." - Doody's Book Review Service
"'Patch-clamp: Methods and Protocols' introduces some common patch-clamp applications. It is intended to assist scientists in identifying problems, which could be best addressed by this technique. The book provides the step-by-step procedures to perform the experiments that answer those questions. ... It will be a useful companion for moderately advanced users wishing to extend their skills." (Maria Drigelova, General Physiology and Biophysics, Vol. 27, April, 2008)
Inhaltsverzeichnis
Table of contents
Part 1. Pharmacology
1. Pharmacological analysis of recombinant NR1a/2A and NR1a/2B NMDA
receptors using whole-cell patch clamp method. Laszlo Fodor and Jozsef
Nagy
2. Memantine as an example of a fast, voltage-dependent, open channel
N-methyl-D-aspartate (NMDA) receptor blocker. Chris G. Parsons and Kate
Gilling
3. Methods for evaluation of positive allosteric modulators of
glutamate AMPA receptors. Edward R. Siuda, Jennifer C. Quirk and Eric
S. Nisenbaum
4. Automated voltage clamp technique. Andrea Ghetti, Antonio Guia and
Jia Xu
5. Flip-the-Tip: automated patch clamping based on glass electrodes.
Michael Fejtl, Uwe Czubayko, Alexander Hümmer, Tobias Krauter and
Albrecht Lepple-Wienhues
6. The Roboocyte: Automated Electrophysiology Based on Xenopus Oocytes.
Christine Leisgen, Mike Kuester and Christoph Methfessel
Part 2. Physiology
7. Infrared guided laser stimulation as a tool for elucidating the
synaptic site of expression of long-term synaptic plasticity. Gerhard
Rammes, Mathias Eder, Walter Zieglgänsberger and Hans-Ulrich Dodt
8. Single-cell RT-PCR, a Technique to Decipher the Electrical,
Anatomical and Genetic Determinants of Neuronal Diversity. Maria
Toledo-Rodriguez and Henry Markram
9. Mechanosensitive Ion Channels investigated simultaneously by
Scanning Probe Microscopy and Patch clamp. Matthias G. Langer
10. Synaptic Connectivity in Engineered Neuronal Networks. Peter
Molnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das and James J.
Hickman
11. Modeling of Action Potential Generation in NG108-15 Cells. Peter
Molnar and James J. Hickman
12. Whole-cell Voltage-clamp on Skeletal Muscle Fibers with the
Silicone-Clamp Technique. Sandrine Pouvreau, Claude Collet, Bruno
Allard and Vincent Jacquemond
13. Determination of channel properties at the unitary level in adult
mammalian isolated cardiomyocytes. RomainGuinamard
14. Electrophysiological Properties of Embryonic Stem Cells During
Differentiation into Cardiomyocyte-like Cell Types. Antoni C.G. van
Ginneken and Arnoud. C. Fijnvandraat
15. Hybrid Neuronal Network Studies Under Dynamic Clamp. Alan D. Dorval
II, Jonathan Bettencourt, Theoden I. Netoff and John A. White
16. Cardiac channelopathies studied with the dynamic action potential
clamp technique. Géza Berecki, Jan G. Zegers, Ronald Wilders and Antoni
C.G. van Ginneken
Part 3. Biophysics
17. Principles of Single-Channel Kinetic Analysis. Feng Qin *
Theoretical chapter
18. Use of Xenopus oocytes to measure ionic selectivity of pore-forming
peptides and ion channels. Thierry Cens and Pierre Charnet
19. Estimation of quantal parameters with multiple-probability
fluctuation analysis. Chiara Saviane and Robin Angus Silver
Klappentext
Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training, and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is organized by the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. Individual techniques are explored within the area that they are applied most often. Researchers will find this text to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies.
Details how electrophysiology can be best utilized in drug discovery and development
Introduces applications of the dynamic clamp method
Includes an extensive theoretical treatise concerning single channel kinetic analysis
