Offers various detailed chapters on experimental deillegalscription, including anecdotal pointers and notes
Intended for Molecular Biologists, Biochemists, and Geneticists
Intended for Molecular Biologists, Biochemists, and Geneticists interested in manipulating and analyzing the Hedgehog Signaling Pathway, this volume offers various detailed chapters on experimental deillegalscription, including anecdotal pointers and notes.
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In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the pathway, which makes it an incredibly important system to understand. This volume examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible and comprehensive text. This volume provides a single source for investigators, presenting several different experimental approaches, which are all varied and broad in nature, as well as procedures for a variety of different model systems. Researchers will find this volume to be an invaluble tool as they work towards deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates.
TABLE OF CONTENTSChapter 1. Purifying the Hedgehog protein and its variants by Darren P.Baker, Frederick R. Taylor and R. Blake PepinskyChapter 2. Application of Sonic hedgehog to the developing chick limbby Eva Tiecke and Cheryll TickleChapter 3. Manipulation of Hedgehog signaling in Xenopus by means ofembryo microinjection and application of chemical inhibitors by ThomasHollemann, Emmanuel Tadjuidje, Katja Koebernick & Tomas PielerChapter 4. Isolation of rat telencephalic neural explants to assay ShhGABAergic interneuron differentiation-inducing activity by Rina Madyand Jhumku D. KohtzChapter 5. Genetic Analysis of the Vertebrate Hedgehog SignalingPathway Using Muscle Cell Fate Specification in the Zebrafish Embryo bySudipto RoyChapter 6. Efficient Manipulation of Hedgehog/GLI Signaling usingRetroviral Expression Systems by Maria Kasper, Gerhard Regl, ThomasEichberger, Anna-Maria Frischauf and Fritz AbergerChapter 7. Cell Surface Marker and Cell Cycle Analysis, HedgehogSignaling, and Flow Cytometry by Kristina Detmer and Ronald E. GarnerChapter 8. Detecting tagged Hedgehog with Intracellular andExtracellular Immunocytochemistry for functional analysis by AinhoaCallejo, Luis Quijada and Isabel GuerreroChapter 9. Confocal Analysis of Hedgehog Morphogenetic Gradient Coupledwith Fluorescent in situ Hybridization of Hedgehog Target Genes byArmel Gallet and Pascal P. ThérondChapter 10. RNAi in the Hedgehog signaling pathway: pFRiPE, a vectorfor temporally and spatially controlled RNAi in Drosophila by EricMarois and Suzanne EatonChapter 11. Germline clone analysis for maternally acting DrosophilaHedgehog components by Erica M. Selva and Beth E. StronachChapter 12. Clonal analysis of Hedgehog signaling in Drosophila somatictissues by Christine M. Bankers and Joan E. HooperChapter 13. GAL4/UAS targeted gene expression for studyingDrosophilaHedgehog signaling by Denise Busson and Anne-Marie PretChapter 14. Biochemical Fractionation of Drosophila Cells by MelanieStegman and David RobbinsChapter 15. Using Immunoprecipitation to Study Protein-proteinInteractions in the Hedgehog Signaling Pathway by Chao Tong and JinJiangChapter 16. Sequence analyses to study the evolutionary history andcis-regulatory elements of Hedgehog genes by Ferenc Müller andAnne-Gaelle Borycki
In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the pathway, which makes it an incredibly important system to understand. This volume examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible and comprehensive text. This volume provides a single source for investigators, presenting several different experimental approaches, which are all varied and broad in nature, as well as procedures for a variety of different model systems. Researchers will find this volume to be an invaluble tool as they work towards deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates.
Purifying the Hedgehog Protein and its Variants.- Application of Sonic Hedgehog to the Developing Chick Limb.- Manipulation of Hedgehog Signaling in Xenopus by Means of Embryo Microinjection and Application of Chemical Inhibitors.- Isolation of Rat Telencephalic Neural Explants to Assay Shh GABAergic Interneuron Differentiation-Inducing Activity.- Genetic Analysis of the Vertebrate Hedgehog-Signaling Pathway Using Muscle Cell Fate Specification in the Zebrafish Embryo.- Efficient Manipulation of Hedgehog/GLI Signaling Using Retroviral Expression Systems.- Cell Surface Marker and Cell Cycle Analysis, Hedgehog Signaling, and Flow Cytometry.- Detecting Tagged Hedgehog with Intracellular and Extracellular Immunocytochemistry for Functional Analysis.- Confocal Analysis of Hedgehog Morphogenetic Gradient Coupled with Fluorescent In Situ Hybridization of Hedgehog Target Genes.- RNAi in the Hedgehog Signaling Pathway: pFRiPE, a Vector for Temporally and Spatially Controlled RNAi in Drosophila.- Germline Clone Analysis for Maternally Acting Drosophila Hedgehog Components.- Clonal Analysis of Hedgehog Signaling in Drosophila Somatic Tissues.- GAL4/UAS Targeted Gene Expression for Studying Drosophila Hedgehog Signaling.- Biochemical Fractionation of Drosophila Cells.- Using Immunoprecipitation to Study Protein-Protein Interactions in the Hedgehog-Signaling Pathway.- Sequence Analyses to Study the Evolutionary History and Cis-Regulatory Elements of Hedgehog Genes.
Inhaltsverzeichnis
TABLE OF CONTENTS
Chapter 1. Purifying the Hedgehog protein and its variants by Darren P.
Baker, Frederick R. Taylor and R. Blake Pepinsky
Chapter 2. Application of Sonic hedgehog to the developing chick limb
by Eva Tiecke and Cheryll Tickle
Chapter 3. Manipulation of Hedgehog signaling in Xenopus by means of
embryo microinjection and application of chemical inhibitors by Thomas
Hollemann, Emmanuel Tadjuidje, Katja Koebernick & Tomas Pieler
Chapter 4. Isolation of rat telencephalic neural explants to assay Shh
GABAergic interneuron differentiation-inducing activity by Rina Mady
and Jhumku D. Kohtz
Chapter 5. Genetic Analysis of the Vertebrate Hedgehog Signaling
Pathway Using Muscle Cell Fate Specification in the Zebrafish Embryo by
Sudipto Roy
Chapter 6. Efficient Manipulation of Hedgehog/GLI Signaling using
Retroviral Expression Systems by Maria Kasper, Gerhard Regl, Thomas
Eichberger, Anna-Maria Frischauf and Fritz Aberger
Chapter 7. Cell Surface Marker and Cell Cycle Analysis, Hedgehog
Signaling, and Flow Cytometry by Kristina Detmer and Ronald E. Garner
Chapter 8. Detecting tagged Hedgehog with Intracellular and
Extracellular Immunocytochemistry for functional analysis by Ainhoa
Callejo, Luis Quijada and Isabel Guerrero
Chapter 9. Confocal Analysis of Hedgehog Morphogenetic Gradient Coupled
with Fluorescent in situ Hybridization of Hedgehog Target Genes by
Armel Gallet and Pascal P. Thérond
Chapter 10. RNAi in the Hedgehog signaling pathway: pFRiPE, a vector
for temporally and spatially controlled RNAi in Drosophila by Eric
Marois and Suzanne Eaton
Chapter 11. Germline clone analysis for maternally acting Drosophila
Hedgehog components by Erica M. Selva and Beth E. Stronach
Chapter 12. Clonal analysis of Hedgehog signaling in Drosophila somatic
tissues by Christine M. Bankers and Joan E. Hooper
Chapter 13. GAL4/UAS targeted gene expression for studyingDrosophila
Hedgehog signaling by Denise Busson and Anne-Marie Pret
Chapter 14. Biochemical Fractionation of Drosophila Cells by Melanie
Stegman and David Robbins
Chapter 15. Using Immunoprecipitation to Study Protein-protein
Interactions in the Hedgehog Signaling Pathway by Chao Tong and Jin
Jiang
Chapter 16. Sequence analyses to study the evolutionary history and
cis-regulatory elements of Hedgehog genes by Ferenc Müller and
Anne-Gaelle Borycki
Klappentext
In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the pathway, which makes it an incredibly important system to understand. This volume examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible and comprehensive text. This volume provides a single source for investigators, presenting several different experimental approaches, which are all varied and broad in nature, as well as procedures for a variety of different model systems. Researchers will find this volume to be an invaluble tool as they work towards deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates.
Offers various detailed chapters on experimental deillegalscription, including anecdotal pointers and notes
Intended for Molecular Biologists, Biochemists, and Geneticists
