A compendium of the most recent and advanced methods applied to the rapidly expanding field of telomerase inhibition
Provides the researcher with a diverse and comprehensive set of tools
This volume presents a compendium of the most recent and advanced methods applied to the rapidly expanding field of telomerase inhibition. The techniques described provide the researcher with a diverse and comprehensive set of tools for the study of telomerase inhibition. The volume is aimed at biochemists, molecular biologists, cancer researchers, and geneticists.
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Due in part to the selective nature of telomerase inhibition as an anticancer approach, the field has expanded considerably in the past decade. The recent advances in methods of telomerase inhibition encompass many different areas of research including molecular biology, cell biology, biochemistry, oncology and gerontology. Telomerase Inhibition provides methods and protocols for those researchers. The techniques described in this book should provide the researcher with a diverse and comprehensive set of tools with which to study telomerase inhibition. Leaders in the field provide recently developed methods that have widespread application such as targeting the telomerase holoenzyme, its RNA template and other elements associated with telomerase activity. Additional methods involving the screening of telomerase inhibitors and telomerase inhibition combined with other chemotherapeutic agents are presented. This text, on the cutting edge of the field, will provide investigators with the most recent methods applied to the expanding field of telomerase inhibition.
Contents:PrefaceContributors1. Methods of Telomerase InhibitionLucy G. Andrews and Trygve O. Tollefsbol2. Telomerase Inhibition by Synthetic Nucleic Acids andChemosensitization in Human Bladder Cancer Cell LinesKai Kraemer, Susanne Fuessel, and Axel Meye3. hTERT Knockdown in Human Embryonic Kidney Cells UsingDouble-Stranded RNASerene R. Lai, Lucy G. Andrews, and Trygve O. Tollefsbol4. RNA Interference Using a Plasmid Construct Expressing Short HairpinRNASerene R. Lai, Lucy G. Andrews, and Trygve O. Tollefsbol5. Retrovirus-mediated RNA Interference: Targeting hTERT Through StableExpression of Short Hairpin RNAAmanda P. Cunningham, Lucy G. Andrews, and Trygve O. Tollefsbol6. Telomerase Inhibition and Telomere Targeting in Hematopoietic CellLines with Small Non-Nucleosidic Synthetic Compounds (BIBR1532)Hesham El Daly and Uwe M. Martens7. Uses of Telomerase Peptides in Anti-tumor Immune TherapyHe Li, Indzi Katik, and Jun-Ping Liu8. Establishing Cell-based Reporter Systems for the Analysis of hTERTexpressionYi-Yuan Huang, Jing-Wen Shih, and Jing-Jer Lin9. Telomerase RNA Inhibition Using Antisense Oligonucleotide AgainstHuman Telomerase RNA Linked to a 2', 5'-Oligoadenylate (2-5A)Yasuko Kondo and Seji Kondo10. Knockdown of Telomerase RNA Using Hammerhead Ribozymes and RNAInterferenceShang Li, Mehdi Nosrati, and Mohammed Kashani-Sabet11. Evaluation of Tankyrase Inhibition in Whole CellsTomohazu Ohishi, Takashi Tsuruo, and Hiroyuki Seimiya12. Inhibition of Telomerase by Targeting MAP Kinase SignalingDakang Xu, He Li, and Jun-Ping Li13. Screening of Telomerase InhibitorsElke Kleideiter, Kamilla Piotrowska, and Ulrich Klotz14. Telomerase Inhibition Combined with Other Chemotherapeutic Reagentsto Enhance Anti-Cancer EffectTetsuzo Tauchi, Junko H. Ohyashiki,and Kazuma Ohyashiki
From the reviews:
"This book contains 14 chapters by active players in the telomerase field. ... Overall this book will be a useful resource for researchers performing telomerase-related experiments." (Lionel Guittat and Jean-Louis Mergny, ChemMedChem, Issue 3, 2008)
Methods of Telomerase Inhibition.- Telomerase Inhibition by Synthetic Nucleic Acids and Chemosensitization in Human Bladder Cancer Cell Lines.- hTERT Knockdown in Human Embryonic Kidney Cells Using Double-Stranded RNA.- RNA Interference Using a Plasmid Construct Expressing Short-Hairpin RNA.- Retrovirus-Mediated RNA Interference.- Telomerase Inhibition and Telomere Targeting in Hematopoietic Cancer Cell Lines with Small Non-Nucleosidic Synthetic Compounds (BIBR1532).- Uses of Telomerase Peptides in Anti-Tumor Immune Therapy.- Establishing Cell-Based Reporter Systems for the Analysis of hTERT Expression.- Telomerase RNA Inhibition Using Antisense Oligonucleotide Against Human Telomerase RNA Linked to a 2?,5?-Oligoadenylate.- Knockdown of Telomerase RNA Using Hammerhead Ribozymes and RNA Interference.- Evaluation of Tankyrase Inhibition in Whole Cells.- Inhibition of Telomerase by Targeting MAP Kinase Signaling.- Screening of Telomerase Inhibitors.- Telomerase Inhibition Combined with Other Chemotherapeutic Reagents to Enhance Anti-Cancer Effect.
From the reviews:
"This book contains 14 chapters by active players in the telomerase field. ... Overall this book will be a useful resource for researchers performing telomerase-related experiments." (Lionel Guittat and Jean-Louis Mergny, ChemMedChem, Issue 3, 2008)
Inhaltsverzeichnis
Contents:
Preface
Contributors
1. Methods of Telomerase Inhibition
Lucy G. Andrews and Trygve O. Tollefsbol
2. Telomerase Inhibition by Synthetic Nucleic Acids and
Chemosensitization in Human Bladder Cancer Cell Lines
Kai Kraemer, Susanne Fuessel, and Axel Meye
3. hTERT Knockdown in Human Embryonic Kidney Cells Using
Double-Stranded RNA
Serene R. Lai, Lucy G. Andrews, and Trygve O. Tollefsbol
4. RNA Interference Using a Plasmid Construct Expressing Short Hairpin
RNA
Serene R. Lai, Lucy G. Andrews, and Trygve O. Tollefsbol
5. Retrovirus-mediated RNA Interference: Targeting hTERT Through Stable
Expression of Short Hairpin RNA
Amanda P. Cunningham, Lucy G. Andrews, and Trygve O. Tollefsbol
6. Telomerase Inhibition and Telomere Targeting in Hematopoietic Cell
Lines with Small Non-Nucleosidic Synthetic Compounds (BIBR1532)
Hesham El Daly and Uwe M. Martens
7. Uses of Telomerase Peptides in Anti-tumor Immune Therapy
He Li, Indzi Katik, and Jun-Ping Liu
8. Establishing Cell-based Reporter Systems for the Analysis of hTERT
expression
Yi-Yuan Huang, Jing-Wen Shih, and Jing-Jer Lin
9. Telomerase RNA Inhibition Using Antisense Oligonucleotide Against
Human Telomerase RNA Linked to a 2', 5'-Oligoadenylate (2-5A)
Yasuko Kondo and Seji Kondo
10. Knockdown of Telomerase RNA Using Hammerhead Ribozymes and RNA
Interference
Shang Li, Mehdi Nosrati, and Mohammed Kashani-Sabet
11. Evaluation of Tankyrase Inhibition in Whole Cells
Tomohazu Ohishi, Takashi Tsuruo, and Hiroyuki Seimiya
12. Inhibition of Telomerase by Targeting MAP Kinase Signaling
Dakang Xu, He Li, and Jun-Ping Li
13. Screening of Telomerase Inhibitors
Elke Kleideiter, Kamilla Piotrowska, and Ulrich Klotz
14. Telomerase Inhibition Combined with Other Chemotherapeutic Reagents
to Enhance Anti-Cancer Effect
Tetsuzo Tauchi, Junko H. Ohyashiki,and Kazuma Ohyashiki
Klappentext
Due in part to the selective nature of telomerase inhibition as an anticancer approach, the field has expanded considerably in the past decade. The recent advances in methods of telomerase inhibition encompass many different areas of research including molecular biology, cell biology, biochemistry, oncology and gerontology. Telomerase Inhibition provides methods and protocols for those researchers. The techniques described in this book should provide the researcher with a diverse and comprehensive set of tools with which to study telomerase inhibition. Leaders in the field provide recently developed methods that have widespread application such as targeting the telomerase holoenzyme, its RNA template and other elements associated with telomerase activity. Additional methods involving the screening of telomerase inhibitors and telomerase inhibition combined with other chemotherapeutic agents are presented. This text, on the cutting edge of the field, will provide investigators with the most recent methods applied to the expanding field of telomerase inhibition.
A compendium of the most recent and advanced methods applied to the rapidly expanding field of telomerase inhibition
Provides the researcher with a diverse and comprehensive set of tools
