Microtubule Protocolsn Editor: Zhou, Jun (Nankai University, Tianjin, China)n n Table of Contents:n n Chapter 1 (review)n Microtubules: an overviewn Richard H. Waden n Chapter 2n Large scale purification of brain tubulin with the modified Weisenberg proceduren José Manuel Andreun n Chapter 3n Purification and mass-spectrometry identification of microtubule-binding proteins from Xenopus egg extractsn Vincent Gache, Patrice Waridel, Sylvie Luche, Andrej Shevchenko and Andrei V. Popovn n Chapter 4n Preparation and characterization of post-translationally modified tubulins from Artemia Franciscanan Paul A. O'Connell and Thomas H. MacRaen n Chapter 5n Studying the structure of microtubules by electron microscopyn Linda A. Amos and Keiko Hirosen n Chapter 6n Fluorescence microscopy of microtubules in cultured cellsn Irina Semenova and Vladimir Rodionovn n Chapter 7 (review)n Measurements of stathmin-tubulin interaction in solutionn Marie-France Carliern n Chapter 8n Analysis of microtubule dynamics by polarized lightn Rudolf Oldenbourgn n Chapter 9n Visualization of spindle behavior using confocal microscopyn Adam I. Marcusn n Chapter 10n Live cell approaches for studying kinetochore-microtubule interactions in Drosophilan Daniel W. Buster and David J. Sharpn n Chapter 11n Analysis of tubulin transport in nerve processesn Andrey Tsvetkov and Sergey Popovn n Chapter 12n Analysis of microtubule-mediated intracellular viral transportn Chunyong Liu, Min Liu and Jun Zhoun n Chapter 13n Xenopus oocyte wound healing as a model system for analysis of microtubule-actin interactionsn Tong Zhang and Craig A. Mandaton n Chapter 14n Screening for inhibitors of microtubule-associated motor proteinsn Frank Kozielski, Salvatore DeBonis and DimitriosSkoufiasn n Chapter 15 (review)n Clinical pharmacology and use of microtubule-targeting agents in cancer therapyn Amelia B. Zelnakn n Chapter 16n Studying drug-tubulin interactions by X-ray crystallographyn Audrey Dorleans, Marcel Knossow and Benoît Gigantn n Chapter 17n Characterizing ligand-microtubule binding by competition methodsn José Fernando Díaz and Rubén Martínez Bueyn n Chapter 18n Methods for studying vinca alkaloid interactions with tubulinn Sharon Lobert and John J. Correian n Chapter 19n High-throughput screening of microtubule-interacting drugsn Susan L. Bane, Rudravajhala Ravindra and Anna A. Zaydmann n Chapter 20 (review)n Strategies for the development of novel taxol-like agentsn Susan L. Mooberry
This book presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs. The book contains a straightforward presentation of readily reproducible protocols, tips for troubleshooting, and advice on avoiding common mistakes. Basic scientists and clinical researchers will benefit from this collection.
Includes methods for the development and discovery of novel agents that target microtubules
Includes readily reproducible protocols, tips for troubleshooting, and advice on avoiding common mistakes