TABLE OF CONTENTSnVol. I Topics: Adenoviruses and vectors, quantitation, and animal modelsnn1. Manipulation of Early Region 4nJulie Boyer and Gary Ketnern2. Isolation, Growth, and Purification of Defective Adenovirus Deletion MutantsnGary Ketner and Julie Boyern3. Construction of Adenovirus Type 5 Early Region 1 (E1) and 4 (E4) Virus MutantsnPeter Groitl and Thomas Dobnern4. Construction of Mouse Adenovirus Type 1 MutantsnAngela N. Cauthen, Amanda R. Welton, and Katherine R. Spindlern5. Generation of Recombinant Adenovirus using E.coli BJ5183 Recombination SystemnP. Seshidhar Reddy, Shanthi Ganesh, Lynda Hawkins, Neeraja Idamakantin6. Production and Release Testing of Ovine AtadenovirusVectors nGerald W. Both, Fiona Cameron, Anne Collins, Linda J. Lockett, Jan Shaw n7. Construction of Capsid Modified Recombinant Bovine Adenovirus Type 3nAlexander N. Zakhartchouk, Qiaohua Wu, Suresh K. Tikoon8. Adenovirus Capsid Chimeras: Fiber Terminal Exon Insertions / Gene Replacements in the MLTUnJason Gall, John Schoggins, and Erik Falck-Pedersenn9. Temperature-Sensitive Replication-Competent Adenovirus shRNA Vectors to Study Cellular Genes in Virus-Induced ApoptosisnT. Subramanian and G. Chinnadurainn10. Evaluating Apoptosis in Tumor Cells Infected with Adenovirus Expressing p53 Tumor Suppressor Gene nElizabeth Perron, M. Behzad Zafar, Amanda Pister, Zhen-Guo Wang, Jun Tian and Prem Sethn11. Growth and Purification of Enteric Adenovirus Type 40nVivien Mautnern12. Immunocompetent, Semi-permissive Cotton Rat Tumor Model for the Evaluation of Oncolytic Adenoviruses nKaroly Toth, Jacqueline F. Spencer, William S.M. Woldn13. The Use of the Syrian Hamster as an Animal Model for Oncolytic Adenovirus VectorsnMaria A. Thomas, Jacqueline F. Spencer, and William S. M. Woldn14. A Real-Time PCR Method to Rapidly Titer Adenovirus StocksnMaria A. Thomas, Drew L. Lichtenstein, Peter Krajcsi, and William S. M. Woldn15. Detection and Quantitation of Subgroup C Adenovirus DNA in Human Tissue Samples by Real-Time PCRnC.T. Garnett, Ching-I Pao, Linda R. Goodingn16. Flow Cytometric Detection of Adenoviruses and Intracellular Adenovirus ProteinsnGraham Bottley, John R. Holt, Nicola J. James and G. Eric Blairn17. Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected OrgansnAmanda R. Welton and Katherine R. Spindlern18. Preparation and Titration of CsCl-Banded Adenovirus Stocks n Ann E. Tollefson, Mohan Kuppuswamy, Elena V. Shashkova, KonstantinnDoronin, and William S. M. Wold
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding the first edition, the authors have added new chapters that address innovative areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers.
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise. The protocols provide a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.