MOLECULAR METHODSn n HEMATOPOIETIC STEM CELL TRANSPLANTATIONn n 1. Molecular profiling of hematopoietic stem cells. (T. V. Bowman , A. A. Merchant, M. Goodell, Houston,Texas, USA)n n 2. Methods for imaging cell fates in hematopoiesis. (J.Duda, M.Karimi, R. Negrin, C. Contag, Stanford, California, USA )n n 3. An overview on HLA typing for hematopoietic stem cell transplantation. (A.M.Little, London, UK). n n 4. Sequence Specific Primed PCR ( PCR-SSP) Typing of HLA Class I and Class II alleles. (K. Dalva, M. Beksac, Ankara, Turkey). n n 5. HLA typing with Sequence Specific Oligonucleotide Primed PCR(PCR-SSOP) and use of the LuminexTM technology. (K. Dalva, M. Beksac, Ankara, Turkey) n n 6. Sequence based HLA typing. (B.Hoppe, A. Salama, Berlin, Germany)n n 7. Typing of minor HLA antigens. (E.Spierings, E. Goulmy, Leiden,NL)n n 8. Detection and role of non-HLA polymorphisms in stem cell transplantation. (Middleton, Newcastle Upon Tyne, UK)n n 9. Polymorphisms within epithelial receptors: NOD2/CARD15. ( J. Brenmoehl, E. Holler, G. Rogler, Germany)n n 10. Role of natural killer cells and killer inhibitor receptor polymorphisms (association of HLA and KIR phenotypes). (M.T. Dorak, Newcastle Upon Tyne, UK)n n 11. Identification of bone marrow derived non-hematopoietic cells by double labeling with immunohistochemistry and in-situ hybridization. (I. Kuzu, M. Beksac, Ankara,Turkey)n n 12. Molecular methods used for detection of minimal residual disease following hematopoietic stem cell transplantation in myeloid disorders. (A. Elmaagacli, Essen, Germany).n n 13. Molecular methods used for the detection of autologous graft contamination in lymphoid disorders. (P. Corradini, M. G. Carrabba, L. Farina, Milan, Italy)n n 14. Detection of graft rejection and relapse by chimerism analysis. (T.Lion, Wien, Austria)n n 15. Application of proteomics to posttransplantation follow-up. (E. M. Weissinger, Hannover,Germany)
This volume is a compendium of cutting-edge molecular methods for the successful transplantation of hematopoietic stem cells. The contributors are world-renown leaders in the field. They describe promising tools for stem cell transplant research models, such as in vivo bioluminescence imaging. They discuss HLA typing, PCR-SSP typing, and HLA antigens. This volume is an invaluable source for biochemists, molecular biologists, and clinicians.
Discussion of HLA typing, PCR-SSP typing, HLA antigens
Chapters written by world-renown leaders in the field