Part I. Measurements of DNA Damage, Cell Death, and Regulators of Cytotoxicityn n In Vitro Micronucleus Technique to Predict Chemosensitivityn Michael Fenechn n Cell Cycle and Drug Sensitivityn Aslamuzzaman Kazi and Q. Ping Doun n TUNEL Assay as a Measure of Chemotherapy-Induced Apoptosisn Robert Wiedern n Apoptosis Assessment by the DNA Diffusion Assayn Narendra P. Singhn n PARP Cleavage and Caspase Activity to Assess Chemosensitivityn Alok C. Bharti, Yasunari Takada, and Bharat B. Aggarwaln n Diphenylamine Assay of DNA Fragmentation for Chemosensitivity Testingn Cicek Gercel-Taylorn n Immunodetecting Members of the Bcl-2 Family of Proteinsn Richard B. Lock and Kathleen M. Murphyn n Correlation of Telomerase Activity and Telomere Length to Chemosensitivityn Yasuhiko Kiyozukan n Application of Silicon Sensor Technologies to Tumor Tissue In Vitro: Detection of Metabolic Correlates of Chemosensitivityn Pedro Mestres-Ventura, Andrea Morguet, Anette Schofer, Michael Laue, and Werner Schmidtn n Overview of Tumor Cell Chemoresistance Mechanismsn Laura Gatti and Franco Zuninon n Flow Cytometric Monitoring of Fluorescent Drug Retention and Effluxn Awtar Krishan and Ronald M. Hamelikn n Flow Cytometric Measurement of Functional and Phenotypic P-Glycoproteinn Monica Pallis and Emma Das-Guptan n Measurement of Ceramide and Sphingolipid Metabolism in Tumors: Potential Modulation of Chemosensitivityn David E. Modrakn n Part II. Genomics, Proteomics, and Chemosensitivityn n Gene Expression Profiling to Characterize Anticancer Drug Sensitivityn James K. Breaux and Gerrit Losn n Identifying Genes Related to Chemosensitivity Using Support Vector Machinen Lei Baon n Genetic Manipulation of Yeast to Identify Genes Involved in Regulation of Chemosensitivityn Giovanni L. Beretta and Paola Peregon n Real-Time RT-PCR (Taqman®) of Tumor mRNA to Predict Sensitivity of Specimens to 5-Fluorouraciln Tetsuro Kubotan n Use of Proteomics to Study Chemosensitivityn Julia Poland, Silke Wandschneider, Andrea Urbani, Sergio Bernardini, Giorgio Federici, and Pranav Sinhan n Part III. In Vivo Animal Modeling of Chemosensitivityn n Clinically Relevant Metastatic Breast Cancer Models to Study Chemosensitivityn Lee Su Kim and Janet E. Pricen n Orthotopic Metastatic (MetaMouse®) Models for Discovery and Development of Novel Chemotherapyn Robert M. Hoffmann n Preclinical Testing of Antileukemic Drugs Using an In Vivo Model of Systemic Diseasen Richard B. Lock, Natalia L. Liem, and Rachael A. Papan n Assessing Growth and Response to Therapy in Murine Tumor Modelsn C. Patrick Reynolds, Bee-Chun Sun, Yves A. DeClerck, and Rex A. Moatsn n Evaluation of Chemosensitivity of Micrometastatses with Green Fluorescent Protein Gene-Tagged Tumor Models in Micen Hayao Nakanishi, Seiji Ito, Yoshinari Mochizuki, and Masae Tatematsun n 99mTc-Annexin A5 Uptake and Imaging to Monitor Chemosensitivityn Tarik Z. Belhocine and Francis G. Blankenbergn n Magnetic Resonance Imaging of Tumor Response to Chemotherapyn Richard Mazurchuk and Joseph A. Spernyakn n Metabolic Monitoring of Chemosensitivity with 18FDG PETn Guy Jerusalem and Tarik Z. Belhocinen n Index
A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity,Volume 2: In Vivo Models, Imaging, and Molecular Regulators contains cutting-edge protocols for classifying tumors into response categories and for customizing therapy to individuals. These readily reproducible techniques allow measurements of DNA damage, apoptotic cell death, and the molecular and cellular regulators of cytotoxicity, as well as in vivo animal modeling of chemosensitivity. A companion volume, Volume 1: In Vitro Assays contains in vitro and in vivo techniques to identify which new agents or combination of agents are effective for each type of tumor.