Table of Contentsn n 1. Non-invasive metabolic assessment of single cellsn David K. Gardner n n 2. Laser Capture Microdissection for Analysis of Single Cells n Nobuki Nakamura, Katharina Ruebel, Long Jin, Xiang Qian, Heyu Zhang, and Ricardo V. Lloydn n 3. Fluorescence in situ hybridization (FISH) on Single Cells (Sex Determination and Chromosome Rearrangements)n Paul N. Scriven and Caroline Mackie Ogilvien n 4. Single cell PCR for PGD: methods, strategies and limitationsn Karen Sermon and Martine De Rycken n 5. Real-Time Quantitative PCR Measurement of Male Fetal DNA in maternal plasman Bernhard Zimmermann, Ahmad El-Sheikhah, Kypros Nicolaides, Wolfgang Holzgreve, Sinuhe Hahnn n 6. Cell-Free Fetal DNA Plasma Extraction and Real Time-PCR Quantificationn Jill L. Maron, Kirby L. Johnson, and Diana W. Bianchin n 7. LATE-PCR and allied technologies: Real-time detection strategies for rapid, reliable diagnosis from single cellsn Kenneth E. Pierce and Lawrence J. Wanghn 8. Efficient isothermal amplification of the entire genome from single cells n Karen V. Schowalter, Jolene R. Fredrickson and Alan R Thornhilln n 9. Comparative Genomic Hybridization on single cellsn Lucille Voullaire and Leeanda Wilton n n 10. Generation of amplified RNAs and cDNA libraries from single mammalian cellsn James Adjayen n 11. Real-time PCR to measure gene expression in single cellsn Dagan Wellsn 12. Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridisation (Array CGH) n Dong Gui Hui, Xin Yuan Guan, and Nicole Husseyn n 13. Microarray technology for mutation analysis of low template DNA samplesn Chelsea Salvado and David Cram
This book applies modern molecular diagnostic techniques to the analysis of single cells, small numbers of cells, or cell extracts. Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reaction and fluorescence in situ hybridization. In particular, this handbook is essential for practitioners providing care for couples seeking treatment for infertility.
Up-to-date, clinically applicable, validated protocols for single-cell diagnostics
Whole genome amplification to create starting material from single cells
Microarrays for analyzing multiple gene sequences simultaneously from a single cell
Non-invasive assessment via polymerase chain reaction and in situ hybridization