Up-to-date, clinically applicable, validated protocols for single-cell diagnostics
Whole genome amplification to create starting material from single cells
Microarrays for analyzing multiple gene sequences simultaneously from a single cell
Non-invasive assessment via polymerase chain reaction and in situ hybridization
This book applies modern molecular diagnostic techniques to the analysis of single cells, small numbers of cells, or cell extracts. Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reaction and fluorescence in situ hybridization. In particular, this handbook is essential for practitioners providing care for couples seeking treatment for infertility.
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Single Cell Diagnostics: Methods and Protocols applies modern
molecular diagnostic techniques to the analysis of single cells, small
numbers of cells, or cell extracts. Emphasis is placed on non-invasive
analysis of single cell metabolites and the direct analysis of RNA and
DNA from single cells, with a focus on polymerase chain reaction and
fluorescence in situ hybridization. The authors, all leading
scientists in the field, provide current and innovative methods for
extracting useful, accurate, and reliable data from single-cell
samples. The stepwise, technically precise protocols presented in this
volume thoroughly describe single-cell assays, from material collection
and isolation of cells to data interpretation. Cell-types analyzed
include embryonic blastomeres, fibroblasts, and lymphocytes. Among the
techniques described are real-time quantitative PCR, isothermal whole
genome amplification, comparative genomic hybridization, real-time
genetic expression analysis, and the production of RNA and cDNA
libraries. This handbook will be essential for researchers and
clinicians, and practitioners providing care for couples seeking
treatment for infertility or preimplantation genetic diagnosis.
Table of Contents 1. Non-invasive metabolic assessment of single cells David K. Gardner 2. Laser Capture Microdissection for Analysis of Single Cells Nobuki Nakamura, Katharina Ruebel, Long Jin, Xiang Qian, Heyu Zhang, and Ricardo V. Lloyd 3. Fluorescence in situ hybridization (FISH) on Single Cells (Sex Determination and Chromosome Rearrangements) Paul N. Scriven and Caroline Mackie Ogilvie 4. Single cell PCR for PGD: methods, strategies and limitations Karen Sermon and Martine De Rycke 5. Real-Time Quantitative PCR Measurement of Male Fetal DNA in maternal plasma Bernhard Zimmermann, Ahmad El-Sheikhah, Kypros Nicolaides, Wolfgang Holzgreve, Sinuhe Hahn 6. Cell-Free Fetal DNA Plasma Extraction and Real Time-PCR Quantification Jill L. Maron, Kirby L. Johnson, and Diana W. Bianchi 7. LATE-PCR and allied technologies: Real-time detection strategies for rapid, reliable diagnosis from single cells Kenneth E. Pierce and Lawrence J. Wangh 8. Efficient isothermal amplification of the entire genome from single cells Karen V. Schowalter, Jolene R. Fredrickson and Alan R Thornhill 9. Comparative Genomic Hybridization on single cells Lucille Voullaire and Leeanda Wilton 10. Generation of amplified RNAs and cDNA libraries from single mammalian cells James Adjaye 11. Real-time PCR to measure gene expression in single cells Dagan Wells 12. Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridisation (Array CGH) Dong Gui Hui, Xin Yuan Guan, and Nicole Hussey 13. Microarray technology for mutation analysis of low template DNA samples Chelsea Salvado and David Cram
From the reviews:
"Single cell diagnostics has become an increasingly important field for both clinicians and researchers who are involved in infertility treatment. ... For researchers this book provides not only detailed state of the art protocols for single cell diagnostics, but also valuable notes on troubleshooting and pitfalls. For clinicians in the field of infertility this nice overview gives an insight into single cell diagnostics and its scientific background. ... This book can be warmly recommended to clinicians and researchers caring for patients with fertility problems." (G. Haidl and J. P. Allam, Andrologia, Vol. 40 (1), 2008)
"This book details innovative methods for analyzing gene expression, chromosome organization, and proteins in single cells and cell extracts. ... The book is written for basic science and clinical researchers, particularly those providing care to couples seeking advice regarding infertility and inherited genetic disorders. ... The chapters are written by experts and the book is carefully edited. ... The laboratory protocols and tips will be valued by laboratory scientists interested in forensic medicine, reproductive medicine, and developmental genetics." (Bruce A. Fenderson, Doody´s Review Service, October, 2007)
The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.
Noninvasive Metabolic Assessment of Single Cells.- Laser Capture Microdissection for Analysis of Single Cells.- Fluorescence In Situ Hybridization on Single Cells.- Single Cell Polymerase Chain Reaction for Preimplantation Genetic Diagnosis.- Real-Time Quantitative Polymerase Chain Reaction Measurement of Male Fetal DNA in Maternal Plasma.- Cell-Free Fetal DNA Plasma Extraction and Real-Time Polymerase Chain Reaction Quantification.- Linear-After-The-Exponential Polymerase Chain Reaction and Allied Technologies.- Efficient Isothermal Amplification of the Entire Genome from Single Cells.- Comparative Genomic Hybridization on Single Cells.- Generation of Amplified RNAs and cDNA Libraries from Single Mammalian Cells.- Use of Real-Time Polymerase Chain Reaction to Measure Gene Expression in Single Cells.- Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridization.- Microarray Technology for Mutation Analysis of Low-Template DNA Samples.
From the reviews:
"Single cell diagnostics has become an increasingly important field for both clinicians and researchers who are involved in infertility treatment. ... For researchers this book provides not only detailed state of the art protocols for single cell diagnostics, but also valuable notes on troubleshooting and pitfalls. For clinicians in the field of infertility this nice overview gives an insight into single cell diagnostics and its scientific background. ... This book can be warmly recommended to clinicians and researchers caring for patients with fertility problems." (G. Haidl and J. P. Allam, Andrologia, Vol. 40 (1), 2008)
"This book details innovative methods for analyzing gene expression, chromosome organization, and proteins in single cells and cell extracts. ... The book is written for basic science and clinical researchers, particularly those providing care to couples seeking advice regarding infertility and inherited genetic disorders. ... The chapters are written by experts and the book is carefully edited. ... The laboratory protocols and tips will be valued by laboratory scientists interested in forensic medicine, reproductive medicine, and developmental genetics." (Bruce A. Fenderson, Doody's Review Service, October, 2007)
Inhaltsverzeichnis
Table of Contentsn n 1. Non-invasive metabolic assessment of single cellsn David K. Gardner n n 2. Laser Capture Microdissection for Analysis of Single Cells n Nobuki Nakamura, Katharina Ruebel, Long Jin, Xiang Qian, Heyu Zhang, and Ricardo V. Lloydn n 3. Fluorescence in situ hybridization (FISH) on Single Cells (Sex Determination and Chromosome Rearrangements)n Paul N. Scriven and Caroline Mackie Ogilvien n 4. Single cell PCR for PGD: methods, strategies and limitationsn Karen Sermon and Martine De Rycken n 5. Real-Time Quantitative PCR Measurement of Male Fetal DNA in maternal plasman Bernhard Zimmermann, Ahmad El-Sheikhah, Kypros Nicolaides, Wolfgang Holzgreve, Sinuhe Hahnn n 6. Cell-Free Fetal DNA Plasma Extraction and Real Time-PCR Quantificationn Jill L. Maron, Kirby L. Johnson, and Diana W. Bianchin n 7. LATE-PCR and allied technologies: Real-time detection strategies for rapid, reliable diagnosis from single cellsn Kenneth E. Pierce and Lawrence J. Wanghn 8. Efficient isothermal amplification of the entire genome from single cells n Karen V. Schowalter, Jolene R. Fredrickson and Alan R Thornhilln n 9. Comparative Genomic Hybridization on single cellsn Lucille Voullaire and Leeanda Wilton n n 10. Generation of amplified RNAs and cDNA libraries from single mammalian cellsn James Adjayen n 11. Real-time PCR to measure gene expression in single cellsn Dagan Wellsn 12. Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridisation (Array CGH) n Dong Gui Hui, Xin Yuan Guan, and Nicole Husseyn n 13. Microarray technology for mutation analysis of low template DNA samplesn Chelsea Salvado and David Cram
Klappentext
The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.
Up-to-date, clinically applicable, validated protocols for single-cell diagnostics
Whole genome amplification to create starting material from single cells
Microarrays for analyzing multiple gene sequences simultaneously from a single cell
Non-invasive assessment via polymerase chain reaction and in situ hybridization
