Quantitative Proteomics by Mass Spectrometry
Editor Salvatore Sechi
Table of Contents
1. Preface by Salvatore Sechi: An Overview of Quantitative Proteomics by Mass Spectrometry.
2. Illarion V. Turko and Salvatore Sechi: Acrylamide - A Cysteine Alkylating Reagent for Quantitative Proteomics.
3. Jeffrey A. Ranish, Marjorie Brand and Ruedi Aebersold: Using stable isotope tagging and MS to characterize protein complexes and to detect changes in their composition.
4. Shao-En Ong and Matthias Mann: Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics.
5. Toshitaka Sato, Yasushi Ishihama, Yoshiya Oda: Quantitative Proteomics of Mouse Brain, and Specific Protein-Interaction Studies using Stable Isotope Labeling.
6. Scott A. Gerber, Arminja N. Kettenbach, John Rush and Steven P. Gygi: The Absolute Quantification Strategy: Application to Phosphorylation Profiling of Human Separase Serine 1126.
7. Sushmita Mimi Roy and Christopher H. Becker: Quantification of proteins and metabolites by mass spectrometry without isotopic labeling.
8. Tao Liu, Wei-Jun Qian, David G. Camp, II, and Richard D. Smith: The Use of a Quantitative Cysteinyl-peptide Enrichment Technology for High-Throughput Quantitative Proteomics.
9. Fred E. Regnier: An isotope coding strategy for proteomics involving both amine and carboxyl group labeling
10. Catherine Fenselau and Xudong Yao: Proteolytic Labeling with 18O for Comparative Proteomics Studies: preparation of 18O-labeled peptides and the 18O/16O peptide mixture
11. Franti_ek Ture_ek, C. Ronald Scott, and Michael H. Gelb: Tandem mass spectrometry in the detection of inborn errors of metabolism and newborn screening.
12. Yu Lu, Patricia Bottari, Ruedi Aebersold, Franti_ek Ture_ek, and Michael H. Gelb: Absolute quantitation of proteins in complex mixtures by the Visible Isotope-Coded Affinity Tagsmethod.
13. Michael J. MacCoss and Christine C. Wu.: Computational Analysis of Quantitative Proteomics Data using Stable Isotope Labeling.
14. Christine C. Wu and Michael J. MacCoss: Quantitative Proteomic Analysis of Mammalian Organisms Using Metabolically Labeled Tissues.
15. Zhang, Y., Wolf-Yadlin A., White F.M.: Quantitative Proteomic Analysis of Phosphotyrosine-Mediated Cellular Signaling Networks.
This volume is a compendium of cutting-edge protocols for quantitative proteomics, and presents the most significant methods used in the field today. The focus on mass spectrometry (MS) is integral. Attention is given to state-of-the-art techniques for the characterization of the phosphoproteome and tandem MS for detection of inborn errors of metabolism. This volume is an indispensable resource in the search for novel biomarkers.
Cutting-edge techniques for introducing isotopic labels
Affinity enrichment of phosphopeptides coupled with stable-isotope labeling
Tandem MS for disease detection
Procedures for characterization of the phosphoproteome
Quantification of proteins by MS without isotopic labels