Development of Improved Strains and Optimization of Fermentation Processesn Lei Han and Sarad R. Parekhn n Experimental Design in Microbiologyn Guillaume E. Vanot and Michelle Sergentn n Metabolic Engineering of Acremonium chrysogenum to Produce Deacetoxycephalosporin C and Bioconversion to 7-Aminodeacetoxycephalosporanic Acidn Marta Rodríguez-Sáiz, Juan-Luis de la Fuente, and José-Luis Barredon n Production of Erythromycin With Saccharopolyspora erythraean Wolfgang Minasn n The Phenylacetyl-CoA Catabolon: Genetic, Biochemical, and Biotechnological Approachesn José M. Luengo, Belén García, Ángel Sandoval, Elsa Arias-Barrau, Sagrario Arias, Francisco Bermejo, and Elías R. Oliveran n Recombinant Microorganisms for the Biosynthesis of Glycosylated Antitumor Compoundsn Carmen Méndez and José A. Salasn n Assay Methods for Detection and Quantification of Antimicrobial Metabolites Produced by Streptomyces clavuligerusn Paloma Liras and Juan F. Martínn n Purification of Plasmid DNA Vectors Produced in Escherichia coli for Gene Therapy and DNA Vaccination Applicationsn Maria Margarida Diogo, João António Queiroz, and Duarte Miguel F. Prazeresn n Genome Breeding of an Amino Acid-Producing Corynebacterium glutamicum Mutantn Masato Ikeda, Junko Ohnishi, and Satoshi Mitsuhashin n Metabolic Activity Profiling by 13C Tracer Experiments and Mass Spectrometry in Corynebacterium glutamicumn Christoph Wittmann and Elmar Heinzlen n Protein and Vitamin Production in Bacillus megateriumn Heiko Barg, Marco Malten, Martina Jahn, and Dieter Jahnn n Strategies for Large-Scale Production of Recombinant Proteins in Filamentous Fungin Heidi Sisniega, José-Luis del Río, María-José Amaya, and Ignacio Fausn n Strain and Culture Conditions Improvement for b-Carotene Production With Mucorn Enrique A. Iturriaga, Tamás Papp, Jesper Breum,José Arnau, and Arturo P. Eslavan n Xanthophylls in Fungi: Metabolic Engineering of the Astaxanthin Biosynthetic Pathway in Xanthophyllomyces dendrorhousn Hans Visser, Gerhard Sandmann, and Jan C. Verdoesn n Methodologies for the Analysis of Fungal Carotenoidsn Paul D. Fraser and Peter M. Bramleyn n Insertional Mutagenesis in the Vitamin B2 Producer Fungus Ashbya gossypiin María A. Santos, Laura Mateos, Karl-Peter Stahmann, and José-Luis Revueltan n Improved Polysaccharide Production Using Strain Improvementn Thomas P. Westn n Use of the Morganella morganii phoC Gene as Reporter in Bacterial and Yeast Hostsn Stefania Cresti, Cesira L. Galeotti, Serena Schippa, Gian Maria Rossolini, and Maria C. Thallern n Gene Expression Arrays in Foodn Bart Weimer, Yi Xie, Lan-szu Chou, and Adele Cutlern n Optimization of Proteome Analysis for Wine Yeast Strainsn Tammi L. Olineka, Apostolos Spiropoulos, Paula A. Mara, and Linda F. Bissonn n Bacteriocin-Producing Strains in a Meat Environmentn Frédéric Leroy and Luc De Vuystn n In Vitro and In Vivo Interactions of Nonpathogenic Bacteria With Immunocompetent Cellsn Eduardo J. Schiffrin, Nabila Ibnou-Zekri, Jean M. Ovigne, Thierry von der Weid, and Stephanie Blumn n Volatile Sulfur Detection in Fermented Foodsn Bart Weimer and Ben Diasn n Bioleaching: Analysis of Microbial Communities Dissolving Metal Sulfidesn Axel Schippers and Klaus Boseckern n Use of PhoN Phosphatase to Remediate Heavy Metalsn Marion Paterson-Beedle and Lynne E. Macaskien n Safe Dispatch and Transport of Biological Materialn Vera Weihs and Christine Rohden n How to Deposit Biological Material for Patent Purposesn Vera Weihsn n Laws and Regulations for the Protection of Biotechnological Inventionsn Uwe Fitznern n Index
The development of biotechnology over the last 20 years, and particularly the use of recombinant DNA techniques, has rapidly expanded the opportu- ties for human benefits from living resources. Efforts to reduce pollution, p- vent environmental damage, combat microbial infection, improve food production, and so on can each involve fermentation or the environmental - lease of microorganisms. Many products of fermentation technology, such as alcoholic beverages, bread, antibiotics, amino acids, vitamins, enzymes, and others, have been influenced by the progress of recombinant DNA techniques. The development of new products or the more efficient manufacturing of those already being produced often involve the use of microorganisms as cell fac- ries for many productions and biotransformations. Microbial Processes and Products is intended to provide practical expe- mental laboratory procedures for a wide range of processes and products me- ated by microorganisms. Although not an exhaustive treatise, it provides a detailed "step-by-step" description of the most recent developments in such applied biotechnological processes. The detailed protocols we provide are cross-referenced in the Notes section, contain critical details, lists of problems and their troubleshooting, as well as safety recommendations that may not n- mally appear in journal articles and can be particularly useful for those un- miliar with specific techniques.