Generation and Use of Transgenic Mice As Models of Osteoarthritisn Anna-Marja Säämänen and Eero Vuorion n Development and Clinical Application in Arthritis of a New Immunoassay for Serum Type IIA Procollagen NH2 Propeptiden Jean-Charles Rousseau, Linda J. Sandell, Pierre D. Delmas, and Patrick Garneron n Histological and Immunohistological Studies on Cartilagen James Melrose, Susan Smith, and Peter Ghoshn n Histochemical Visualization of the Cartilage Hyaladherins Using a Biotinylated Hyaluronan Oligosaccharide Bioaffinity Proben James Melrose and Susan Smithn n Methods for Cartilage and Subchondral Bone Histomorphometryn Philippe Pastoureau and Agnès Chomeln n Laser-Mediated Microdissection As a Tool for Molecular Analysis in Arthritisn Martin Judex, Elena Neumann, Steffen Gay, and Ulf Müller-Ladnern n Analysis of Protein Distribution in Cartilage Using Immunofluorescence and Laser Confocal Scanning Microscopyn Stephan Soeder, Alexander Kuhlmann, and Thomas Aignern n Molecular and Biochemical Assays of Cartilage Componentsn Caroline D. Hoemannn n Mechanical Characterization of Native and Tissue-Engineered Cartilagen Albert C. Chen, Stephen M. Klisch, Won C. Bae, Michele M. Temple, Kevin B. McGowan, Kenneth R. Gratz, Barbara L. Schumacher, and Robert L. Sahn n Noninvasive Study of Human Cartilage Structure by MRIn Felix Ecksteinn n High-Resolution MRI Techniques for Studies in Small-Animal Modelsn Olivier Beufn n High-Resolution Imaging of Osteoarthritis Using Microcomputed Tomographyn Lydia Wachsmuth and Klaus Engelken n High-Resolution Ultrasonography for Analysis of Age- and Disease-Related Cartilage Changesn Amena Saïed and Pascal Laugiern n Evaluation of Cartilage Composition and Degradation by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonancen Jürgen Schiller, Daniel Huster, BeateFuchs, Lama Naji, Jörn Kaufmann, and Klaus Arnoldn n Pulsed-Field Gradient-Nuclear Magnetic Resonance (PFG NMR) to Measure the Diffusion of Ions and Polymers in Cartilage: Applications in Joint Diseasesn Jürgen Schiller, Lama Naji, Robert Trampel, Wilfred Ngwa, Robert Knauss, and Klaus Arnoldn n Dynamics of Collagen in Articular Cartilage Studied by Solid-State NMR Methodsn Daniel Huster, Jürgen Schiller, and Klaus Arnoldn n Computerized Protocol for Anatomical and Functional Studies of Jointsn Sandra Martelli and Stefano Zaffagninin n Index
An indispensable collection of updated classical and emerging techniques that promise to add critical knowledge to our understanding of cartilage metabolism in health and disease. Volume 1: Cellular and Molecular Tools describes proven molecular and cellular techniques for the in vitro study of normal and osteoarthritic cartilage through biochemical, biomolecular, immunological, and physical approaches, with emphasis on the genetic manipulation of cells. Volume 2: Structure and In Vivo Analysis, offers cutting-edge procedures for the study-at the tissue level-of turnover, structure, and functioning of normal and diseased articular cartilage by invasive and noninvasive means. Comprehensive and up-to-date, the two volumes of Cartilage and Osteoarthritis provide researchers and bench scientists with readily reproducible protocols for new experiments to understand-from the cellular to the animal level-the pathophysiology of cartilage and to discover molecular targets for pharmacological intervention.