Isolation of RNA and DNA From Leukocytes and cDNA Synthesisn Joop H. Jansen and Bert A. van der Reijdenn n Cytogenetic and FISH Techniques in Myeloid Malignanciesn Lynda J. Campbelln n Overview of Real-Time RT-PCR Strategies for Quantification of Gene Rearrangements in the Myeloid Malignanciesn Christophe Picard, Monique Silvy, and Jean Gabertn n Diagnosis and Monitoring of Chronic Myeloid Leukemia by Qualitative and Quantitative RT-PCRn Susan Branford and Timothy Hughesn n Detection of BCR-ABL Mutations and Resistance to Imatinib Mesylaten Susan Branford and Timothy Hughesn n Deletion of the Derivative Chromosome 9 in Chronic Myeloid Leukemian Lynda J. Campbelln n Diagnosis and Monitoring of PML-RARA-Positive Acute Promyelocytic Leukemia by Qualitative RT-PCRn Vincenzo Rossi, Laura Levati, and Andrea Biondin n Diagnosis and Monitoring of PML-RARa-Positive Acute Promyelocytic Leukemia by Quantitative RT-PCRn Elisa Mokany, Alison V. Todd, Caroline J. Fuery, and Tanya L. Applegaten n Diagnosis and Monitoring of AML1-MTG8 (ETO)-Positive Acute Myeloid Leukemia by Qualitative and Real-Time Quantitative RT-PCRn Khalid Tobal and John A. Liu Yinn n Diagnosis and Monitoring of CBFB-MYH11-Positive Acute Myeloid Leukemia by Qualitative and Quantitative RT-PCRn Bert A. van der Reijden and Joop H. Jansenn n Detection of the FIP1L1-PDGFRA Fusion in Idiopathic Hypereosinophilic Syndrome and Chronic Eosinophilic Leukemian Jan Cools, Elizabeth H. Stover, and D. Gary Gillilandn n FLT3 Mutations in Acute Myeloid Leukemian Hitoshi Kiyoi and Tomoki Naoen n WT-1 Overexpression in Acute Myeloid Leukemia and Myelodysplastic Syndromesn Daniela Cilloni, Enrico Gottardi, and Giuseppe Saglion n Classification of AML by DNA-Oligonucleotide Microarraysn Alexander Kohlmann, Wolfgang Kern, Wolfgang Hiddemann, and Torsten Haferlachn n Classification of AML Using a Monoclonal Antibody Microarrayn Richard I. Christopherson, Kerryn Stoner, Nicole Barber, Larissa Belov, Adrian Woolfson, Mike Scott, Linda Bendall, and Stephen P. Mulligann n Methods for the Detection of the JAK2 V617F Mutation in Human Myeloproliferative Disordersn Peter J. Campbell, Linda M. Scott, E. Joanna Baxter, Anthony J. Bench, Anthony R. Green, and Wendy N. Erbern n Overexpression of PRV-1 Gene in Polycythemia Rubra Vera and Essential Thrombocythemian Maurizio Martini, Luciana Teofili, and Luigi M. Laroccan n Chimerism Analysis Following Nonmyeloablative Stem Cell Transplantationn Thomas Lion and Franz Watzingern n Index
A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.