Biological and Clinical Basis for Molecular Studies of Interferonsn Katie R. Pang, Jashin J. Wu, David B. Huang, Stephen K. Tyring, and Samuel Baronn n Interferon Research: A Brief Historyn Myriam S. Kunzi and Paula M. Pithan n Virus Infection and the Interferon Response: A Global View Through Functional Genomicsn Marcus J. Korth, John C. Kash, Jeffrey C. Furlong, and Michael G. Katzen n Genomic DNA Affinity Chromatography: A Technique to Isolate Interferon-Inducible DNA Binding Factorsn Jyothi Kumaran and Eleanor N. Fishn n Protein Engineering of Interferon Alphasn Renqiu Hu, Ke-jian Lei, Joseph Bekisz, and Kathryn C. Zoonn n Assays for the Interferon-Induced Enzyme 2',5' Oligoadenylate Synthetasesn Saumendra N. Sarkar, Mitali Pandey, and Ganes C. Senn n A Convenient and Sensitive Fluorescence Resonance Energy Transfer Assay for RNase L and 2',5' Oligoadenylatesn Chandar S. Thakur, Zan Xu, Zhengfu Wang, Zachary Novince, and Robert H. Silvermann n Regulation of Murine Interferon Regulatory Factor Gene Expression in the Central Nervous System Determined by Multiprobe RNase Protection Assayn Shalina S. Ousman and Iain L. Campbelln n Mitogen-Activated Protein Kinase Pathways in Interferon Signalingn Antonella Sassano, Amit Verma, and Leonidas C. Plataniasn n Development of an Interferon-Based Cancer Vaccine Protocol: Application to Several Types of Murine Cancersn W. Robert Fleischmann, Jr. and Tzu G. Wun n Type I Interferons as Regulators of the Differentiation/Activation of Human Dendritic Cells: Methods for the Evaluation of IFN-Induced Effectsn Stefano M. Santini, Caterina Lapenta, and Filippo Belardellin n Flow Cytometric Techniques for Studying Plasmacytoid Dendritic Cells in Mixed Populationsn Stacey L. Olshalsky and Patricia Fitzgerald-Bocarslyn n Analysis of Anti-Interferon Properties of the Herpes Simplex Virus Type IICP0 Proteinn Karen Mossmann n Interferon Subtype Gene Therapy for Regulating Cytomegalovirus Diseasen Cassandra M. James, Emmalene J. Bartlett, Josephine P. Mansfield, and Vanessa S. Culln n Transfection of Müller Cells With Type I Interferon Transgenes: Resistance to HSV-1 Infectionn Benitta John-Philip and Daniel J. J. Carrn n Index
A compendium of optimized methods to measure type I interferon efficacy as an antiproliferative or an antiviral agent. These cutting-edge techniques range from the simple to the highly complex and serve to illuminate the signaling cascades and the activation of enzymatic pathways prompted by interferon. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Interferon Methods and Protocols offers researchers powerful tools not only to ascertain the functions of IFN-stimulatory gene products, but also to identify additional molecular pathways that will clarify our understanding of the many biological events influenced by IFNs.