Multiple Myeloma: Challenges and Opportunitiesn Douglas Joshua, Ross D. Brown, and P. Joy Hon n Immunophenotyping of Plasma Cells in Multiple Myeloman Gema Mateo Manzanera, Jesús F. San Miguel Izquierdo, and Alberto Orfao de Matosn n Plasma Cell Labeling Indexn Philip R. Greipp and Shaji Kumarn n Conventional Cytogenetics in Myeloman Lynda J. Campbelln n Multicolor Spectral Karyotyping in Multiple Myeloman Jeffrey R. Sawyern n Detection of Chromosome 13 Deletions by Fluorescent In Situ Hybridizationn Scott VanWier and Rafael Fonsecan n Comparative Genomic Hybridization for Analysis of Changes in DNA Copy Number in Multiple Myeloman Pulivarthi H. Raon n Southern Blotting of IgH Rearrangements in B-Cell Disordersn Edna Yuen and Ross D. Brownn n Identification and Assembly of V Genes As Idiotype-Specific DNA Fusion Vaccines in Multiple Myeloman Surinder S. Sahota, Mark Townsend, and Freda K. Stevensonn n Identification of Clonotypic IgH VDJ Sequences in Multiple Myeloman Brian J. Taylor, Jitra Kriangkum, Erin R. Strachan, Juanita Wizniak, and Linda M. Pilarskin n Real-Time Polymerase Chain Reaction of Immunoglobulin Rearrangements for Quantitative Evaluation of Minimal Residual Disease in Myeloman Mara Compagno, Barbara Mantoan, Monica Astolfi, Mario Boccadoro, and Marco Ladetton n Incomplete DJH Rearrangementsn David González and Ramón Garcia-Sanzn n Identification of Malignant Plasma Cells by mRNA In Situ Hybridizationn Ross D. Brown and P. Joy Hon n The SCID-hu Myeloma Modeln Joshua Epstein and Shmuel Yaccobyn n The 5T2MM Murine Model of Multiple Myeloma: Maintenance and Analysisn Karin Vanderkerken, Kewal Asosingh, Angelo Willems, Hendrik De Raeve, Pedro Couck, Frans Gorus, Peter Croucher, and Ben Van Campn n Determination of Telomerase Activity and Telomere Lengthn Kai-da Wu andMalcolm A. S. Mooren n Dendritic Cell/Myeloma Hybrid Vaccinen Dajing Xia, Tim Chan, and Jim Xiangn n Genetically Engineered Myeloma Cell Vaccinen Siguo Hao, Tim Chan, and Jim Xiangn n An In Vitro Osteoclast-Forming Assay to Measure Myeloma Cell-Derived Osteoclast-Activating Factorsn Andrew C. W. Zannettino, Amanda N. Farrugia, L. Bik To, and Gerald J. Atkinsn n Clonality Detection of the Expanded T-Cell Populations in Patients With Multiple Myeloman Daniel M-Y. Szen n Detecting Mismatch Repair Defects in Myeloman Mark Velangi, Elizabeth Matheson, Andrew Hall, and Julie Irvingn n Methylation-Specific Polymerase Chain Reactionn Oliver Galm and James G. Hermann n Making Sense of DNA Microarray Datan Wei-min Liu, Sunhee K. Ro, and Walter H. Kochn n Index
A comprehensive collection of classic and innovative methodologies used in many laboratories for the investigation of multiple myeloma. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. These proven techniques are ideal for studying the pathogenesis of multiple myeloma and identifying new therapeutic targets.