Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995.
This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Detailed descriptions of materials required and stepwise protocols are presented for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.
Contents
Preface
Contributors
Chapter 1
Long-term ex situ conservation of Biological Resources and the role of Biological Resource Centres
Glyn N. Stacey and John G. Day
Chapter 2
The Process of Freeze-drying
Gerald Adams
Chapter 3
Principles of Cryopreservation
David E. Pegg
Chapter 4
Lyophilization of Proteins
Paul Matejschuk
Chapter 5
Vacuum-drying and Cryopreservation of Prokaryotes
Brian J. Tindall
Chapter 6
Freeze-drying of Yeast Cultures
Chris Bond
Chapter 7
Cryopreservation of Yeast Cultures
Chris Bond Chapter 8
Freeze-drying Fungi using a Shelf-freeze-drier
C. Shu-hui Tan, Cor W. van Ingen and Joost A. Stalpers
Chapter 9
Cryopreservation and Freeze-drying of Fungi employing Centrifugal and Shelf Freeze-drying
Matthew J. Ryan and David Smith
Chapter 10
Cryopreservation of Microalgae and Cyanobacteria
John G. Day
Chapter 11
Cryopreservation of Plant Cell Suspensions
Brian W. W. Grout
Chapter 12
Cryopreservation of Shoot-tips and Meristems
Erica E. Benson, Keith Harding and Jason W. Johnston
Chapter 13
Cryopreservation of Desiccation Tolerant Seeds
Hugh W. Pritchard
Chapter 14
Cryopreservation of Fish Sperm
Eugeny Kopeika, Julia Kopeika and Tiantian Zhang
Chapter 15
Cryopreservation of Avian Spermatozoa
Graham Wishart
Chapter 16
Cryopreservation of Animal and Human Cell Lines
Christopher B. Morris
Chapter 17
Cryopreservation of Haematopoietic Stem/Progenitor Cells for Therapeutic Use
Suzanne M. Watt, Eric Austin and Sue Armitage
Chapter 18
Cryopreservation of Human Embryonic Stem Cell Lines
Charles J. Hunt and Paula M. Timmons
Chapter 19
Cryopreservation of Primary Animal Cell Cultures
Glyn N. Stacey and Stuart Dowall
Chapter 20
Cryopreservation of Red Blood Cells and Platelets
Andreas Sputtek
Chapter 21
Cryopreservation of Mammalian Semen
Mark R. Curry
Chapter 22
Cryopreservation of Mammalian Oocytes
Sharon J. Paynter and Barry J. Fuller
Chapter 23
Cryopreservation of Mammalian Embryos
Barry J. Fuller and Sharon J. Paynter
Glossary
From the reviews of the second edition:
"...practical and reliable resource for the conservation and sustainable use of biological resources."-Anticancer Research
"This book is a welcome addition to the relatively sparse comprehensive reference publications on preservation methodologies which are currently available. It begins sensibly with a grounding in the principles involved in preservation, in logical and easy to follow steps. It is well laid out and covers a wide spectrum of cell/organism types in the one volume ... ." (Peter Green, Molecular Biotechnology, December, 2007)
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Inhaltsverzeichnis
Contents
Preface
Contributors
Chapter 1
Long-term ex situ conservation of Biological Resources and the role of Biological Resource Centres
Glyn N. Stacey and John G. Day
Chapter 2
The Process of Freeze-drying
Gerald Adams
Chapter 3
Principles of Cryopreservation
David E. Pegg
Chapter 4
Lyophilization of Proteins
Paul Matejschuk
Chapter 5
Vacuum-drying and Cryopreservation of Prokaryotes
Brian J. Tindall
Chapter 6
Freeze-drying of Yeast Cultures
Chris Bond
Chapter 7
Cryopreservation of Yeast Cultures
Chris Bond
Chapter 8
Freeze-drying Fungi using a Shelf-freeze-drier
C. Shu-hui Tan, Cor W. van Ingen and Joost A. Stalpers
Chapter 9
Cryopreservation and Freeze-drying of Fungi employing Centrifugal and Shelf Freeze-drying
Matthew J. Ryan and David Smith
Chapter 10
Cryopreservation of Microalgae and Cyanobacteria
John G. Day
Chapter 11
Cryopreservation of Plant Cell Suspensions
Brian W. W. Grout
Chapter 12
Cryopreservation of Shoot-tips and Meristems
Erica E. Benson, Keith Harding and Jason W. Johnston
Chapter 13
Cryopreservation of Desiccation Tolerant Seeds
Hugh W. Pritchard
Chapter 14
Cryopreservation of Fish Sperm
Eugeny Kopeika, Julia Kopeika and Tiantian Zhang
Chapter 15
Cryopreservation of Avian Spermatozoa
Graham Wishart
Chapter 16
Cryopreservation of Animal and Human Cell Lines
Christopher B. Morris
Chapter 17
Cryopreservation of Haematopoietic Stem/Progenitor Cells for Therapeutic Use
Suzanne M. Watt, Eric Austin and Sue Armitage
Chapter 18
Cryopreservation of Human Embryonic Stem Cell Lines
Charles J. Hunt and Paula M. Timmons
Chapter 19
Cryopreservation of Primary Animal Cell Cultures
Glyn N. Stacey and Stuart Dowall
Chapter 20
Cryopreservation of Red Blood Cells and Platelets
Andreas Sputtek
Chapter 21
Cryopreservation of Mammalian Semen
Mark R. Curry
Chapter 22
Cryopreservation of Mammalian Oocytes
Sharon J. Paynter and Barry J. Fuller
Chapter 23
Cryopreservation of Mammalian Embryos
Barry J. Fuller and Sharon J. Paynter
Glossary
Klappentext
This widely expanded second edition offers a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. It includes novel approaches and protocols that were not preservable when the first edition was published.
The book begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures.
This widely expanded second edition offers a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. It includes novel approaches and protocols that were not preservable when the first edition was published.
The book begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Coverage presents detailed descriptions of the materials required and stepwise protocols for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.
