Includes supplementary material: sn.pub/extras
A cutting-edge collection of readily reproducible techniques for the isolation, culture, and study of activation and signaling in human mast cells. These methods take advantage of the latest advances in molecular biology, technology, and information science. They include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis.
Part I. Overview of Mast Cell Biology Paul Ehrlich's 'Mastzellen'-From Aniline Dyes to DNA Chip Arrays: A Historical Review of Developments in Mast Cell Research Harsha Vyas and Guha Krishnaswamy The Human Mast Cell: An Overview Guha Krishnaswamy, Omar Ajitawi, and David S. Chi Mast Cells in Allergy and Autoimmunity: Implications for Adaptive Immunity Gregory D. Gregory and Melissa A. Brown Part II. Identification of Mast Cells in Culture and in Tissue Analysis of MCT and MCTC Mast Cells in Tissue Lawrence B. Schwartz Mast Cell Ultrastructure and Staining in Tissue Shruti A. Shukla, Ranjitha Veerappan, Judy S. Whittimore, Lou Ellen Miller, and George A. Youngberg Expression of Cell Surface Antigens on Mast Cells: Mast Cell Phenotyping Alexander W. Hauswirth, Stefan Florian, Gerit-Holger Schernthaner, Maria-Theresa Krauth, Karoline Sonneck, Wolfgang R. Sperr, and Peter Valent Identification of Mast Cells in the Cellular Response to Myocardial Infarction Nikolaos G. Frangogiannis and Mark L. Entman Part III. Development of Mast Cells In Vitro Growth of Human Mast Cells From Bone Marrow and Peripheral Blood-Derived CD34+ Pluripotent Progenitor Cells Arnold S. Kirshenbaum and Dean D. Metcalfe Culture of Human Mast Cells From Hemopoietic Progenitors Hirohisa Saito Isolation, Culture, and Characterization of Intestinal Mast Cells Gernot Sellge and Stephan C. Bischoff Part IV. Mast Cell Signaling and Gene Expression Activation of Nuclear Factor-kB Chuanfu Li, Jim Kelley, and Tuanzhu Ha Analysis of Mitogen-Activated Protein Kinase Activation Stephen C. Armstrong Microarray and Gene-Clustering Analysis Tsung-Hsien Tsai, Denise M. Milhorn, and Shau-Ku Huang Techniques to Study FceRI Signaling Yuko Kawakami, Jiro Kitaura, and Toshiaki Kawakami Part V. Mast Cell Expression and Release of Inflammatory Mediators Human Mast Cell Proteases: Activity Assays Using Thiobenzyl Ester Substrates David A. Johnson Mast Cell Histamine and Cytokine Assays David S. Chi, S. Matthew Fitzgerald, and Guha Krishnaswamy Measurement of Mast Cell Cytokine Release by Multiplex Assay Kevin F. Breuel and W. Keith De Ponti Assays for Histamine-Releasing Factors: From Identification and Cloning to Discovery of Binding Partners Jacqueline M. Langdon and Susan M. MacDonald Assays for Nitric Oxide Expression William L. Stone, Hongsong Yang, and Min Qui Immunohistological Detection of Growth Factors and Cytokines in Tissue Mast Cells Zhenhong Qu Part VI. Mast Cell Interactions With Other Cell Types Endothelial Cell Activation by Mast Cell Mediators Kottarappat N. Dileepan and Daniel J. Stechschulte Co-Culture of Mast Cells With Fibroblasts: A Tool to Study Their Crosstalk Ido Bachelet, Ariel Munitz, and Francesca Levi-Schaffer Detection of e Class Switching and IgE Synthesis in Human B Cells Jérôme Pène, Florence Guilhot, Isabelle Cognet, Paul Guglielmi, Angélique Guay-Giroux, Jean-Yves Bonnefoy, Greg C. Elson, Hans Yssel, and Jean-François Gauchat Part VII. Novel Aspects of Mast Cell Activation and Regulation Gene Silencing Using Small Interference RNA in Mast Cells Deling Yin and Charles A. Stuart Mast Cell Activation by Lipoproteins Jim Kelley, Gregory Hemontolor, Walid Younis, Chuanfu Li, Guha Krishnaswamy, and David S. Chi Mast Cell Activation by Stress Ann L. Baldwin Part VIII. Roles of Mast Cells in Host Defense In Vivo Models for Studying Mast Cell-Dependent Responses to Bacterial Infection Christopher P. Shelburne, James B. McLachlan, and Soman N.
The mast cell, long implicated in causing allergic reactions, may also be involved in many other disease processes, including cancer, heart disease, parasitic disease, atherosclerosis, asthma, and arthritis. In Mast Cells: Methods and Protocols, hands-on experts describe in detail their best techniques for the isolation, culture, and study of both activation and signaling in human mast cells. These readily reproducible methods take advantage of the latest advances in molecular biology, technology, and information science. The techniques provide a sound base of methodology for mast cell research and include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis. A survey of mast cell biology offers insight into its history and the implications for adaptive immunity. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Mast Cells: Methods and Protocols provides mast cell researchers with reproducible accounts of basic and advanced molecular and cellular techniques used in studying this fascinating, multifunctional cell.
From the reviews:
"This volume provides a set of protocols for investigators working with mast cells. Each of the chapters is accompanied by a short bibliography. ... The information will be of value to advanced students and laboratory investigators. ... Each of the substantive chapters contains detailed protocols and a short set of primary references. ... there is sufficient detail to allow direct adaptation in the laboratory. ... the volume contains useful material for investigators working in this area." (Eugene A Davidson, Doody´s Book Review Service, March, 2006)
A cutting-edge collection of readily reproducible techniques for the isolation, culture, and study of activation and signaling in human mast cells. These methods take advantage of the latest advances in molecular biology, technology, and information science. They include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis. The mast cell, long implicated in causing allergic reactions, may also be involved in many other disease processes, including cancer, heart disease, parasitic disease, atherosclerosis, asthma, and arthritis. In Mast Cells: Methods and Protocols, hands-on experts describe in detail their best techniques for the isolation, culture, and study of both activation and signaling in human mast cells. These readily reproducible methods take advantage of the latest advances in molecular biology, technology, and information science. The techniques provide a sound base of methodology for mast cell research and include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis. A survey of mast cell biology offers insight into its history and the implications for adaptive immunity. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Mast Cells: Methods and Protocols provides mast cell researchers with reproducible accounts of basic and advanced molecular and cellular techniques used in studying this fascinating, multifunctional cell.
Overview of Mast Cell Biology.- Paul Ehrlich''s ";Mastzellen"- From Aniline Dyes to DNA Chip Arrays.- The Human Mast Cell.- Mast Cells in Allergy and Autoimmunity.- Identification of Mast Cells in Culture and in Tissue.- Analysis of MCT and MCTC Mast Cells in Tissue.- Mast Cell Ultrastructure and Staining in Tissue.- Expression of Cell Surface Antigens on Mast Cells.- Identification of Mast Cells in the Cellular Response to Myocardial Infarction.- Development of Mast Cells In Vitro.- Growth of Human Mast Cells From Bone Marrow and Peripheral Blood-Derived CD34+ Pluripotent Progenitor Cells.- Culture of Human Mast Cells From Hemopoietic Progenitors.- Isolation, Culture, and Characterization of Intestinal Mast Cells.- Mast Cell Signaling and Gene Expression.- Activation of Nuclear Factor-?B.- Analysis of Mitogen-Activated Protein Kinase Activation.- Microarray and Gene-Clustering Analysis.- Techniques to Study Fc?RI Signaling.- Mast Cell Expression and Release of Inflammatory Mediators of Inflammatory Mediators.- Human Mast Cell Proteases.- Mast Cell Histamine and Cytokine Assays.- Measurement of Mast Cell Cytokine Release by Multiplex Assay.- Assays for Histamine-Releasing Factors.- Assays for Nitric Oxide Expression.- Immunohistological Detection of Growth Factors and Cytokines in Tissue Mast Cells.- Mast Cell Interactions With Other Cell Types.- Endothelial Cell Activation by Mast Cell Mediators.- Co-Culture of Mast Cells With Fibroblasts.- Detection of ? Class Switching and IgE Synthesis in Human B Cells.- Novel Aspects of Mast Cell Activation and Regulation.- Gene Silencing Using Small Interference RNA in Mast Cells.- Mast Cell Activation by Lipoproteins.- Mast Cell Activation by Stress.- Roles of Mast Cells in Host Defense.- In Vivo Models for StudyingMast Cell-Dependent Responses to Bacterial Infection.- Bacterial Activation of Mast Cells.- Activation of Mast Cells by Streptolysin O and Lipopolysaccharide.- Analysis of Programmed Cell Death in Mast Cells.- Mast Cell Apoptosis.
From the reviews:
"This volume provides a set of protocols for investigators working with mast cells. Each of the chapters is accompanied by a short bibliography. ... The information will be of value to advanced students and laboratory investigators. ... Each of the substantive chapters contains detailed protocols and a short set of primary references. ... there is sufficient detail to allow direct adaptation in the laboratory. ... the volume contains useful material for investigators working in this area." (Eugene A Davidson, Doody's Book Review Service, March, 2006)
Inhaltsverzeichnis
Part I. Overview of Mast Cell Biology
Paul Ehrlich's 'Mastzellen'-From Aniline Dyes to DNA Chip Arrays: A Historical Review of Developments in Mast Cell Research
Harsha Vyas and Guha Krishnaswamy
The Human Mast Cell: An Overview
Guha Krishnaswamy, Omar Ajitawi, and David S. Chi
Mast Cells in Allergy and Autoimmunity: Implications for Adaptive Immunity
Gregory D. Gregory and Melissa A. Brown
Part II. Identification of Mast Cells in Culture and in Tissue
Analysis of MCT and MCTC Mast Cells in Tissue
Lawrence B. Schwartz
Mast Cell Ultrastructure and Staining in Tissue
Shruti A. Shukla, Ranjitha Veerappan, Judy S. Whittimore, Lou Ellen Miller, and George A. Youngberg
Expression of Cell Surface Antigens on Mast Cells: Mast Cell Phenotyping
Alexander W. Hauswirth, Stefan Florian, Gerit-Holger Schernthaner, Maria-Theresa Krauth, Karoline Sonneck, Wolfgang R. Sperr, and Peter Valent
Identification of Mast Cells in the Cellular Response to Myocardial Infarction
Nikolaos G. Frangogiannis and Mark L. Entman
Part III. Development of Mast Cells In Vitro
Growth of Human Mast Cells From Bone Marrow and Peripheral Blood-Derived CD34+ Pluripotent Progenitor Cells
Arnold S. Kirshenbaum and Dean D. Metcalfe
Culture of Human Mast Cells From Hemopoietic Progenitors
Hirohisa Saito
Isolation, Culture, and Characterization of Intestinal Mast Cells
Gernot Sellge and Stephan C. Bischoff
Part IV. Mast Cell Signaling and Gene Expression
Activation of Nuclear Factor-kB
Chuanfu Li, Jim Kelley, and Tuanzhu Ha
Analysis of Mitogen-Activated Protein Kinase Activation
Stephen C. Armstrong
Microarray and Gene-Clustering Analysis
Tsung-Hsien Tsai, Denise M. Milhorn, and Shau-Ku Huang
Techniques to Study FceRI Signaling
Yuko Kawakami, Jiro Kitaura, and Toshiaki Kawakami
Part V. Mast Cell Expression and Release of Inflammatory Mediators
Human Mast Cell Proteases: Activity Assays Using Thiobenzyl Ester Substrates
David A. Johnson
Mast Cell Histamine and Cytokine Assays
David S. Chi, S. Matthew Fitzgerald, and Guha Krishnaswamy
Measurement of Mast Cell Cytokine Release by Multiplex Assay
Kevin F. Breuel and W. Keith De Ponti
Assays for Histamine-Releasing Factors: From Identification and Cloning to Discovery of Binding Partners
Jacqueline M. Langdon and Susan M. MacDonald
Assays for Nitric Oxide Expression
William L. Stone, Hongsong Yang, and Min Qui
Immunohistological Detection of Growth Factors and Cytokines in Tissue Mast Cells
Zhenhong Qu
Part VI. Mast Cell Interactions With Other Cell Types
Endothelial Cell Activation by Mast Cell Mediators
Kottarappat N. Dileepan and Daniel J. Stechschulte
Co-Culture of Mast Cells With Fibroblasts: A Tool to Study Their Crosstalk
Ido Bachelet, Ariel Munitz, and Francesca Levi-Schaffer
Detection of e Class Switching and IgE Synthesis in Human B Cells
Jérôme Pène, Florence Guilhot, Isabelle Cognet, Paul Guglielmi, Angélique Guay-Giroux, Jean-Yves Bonnefoy, Greg C. Elson, Hans Yssel, and Jean-François Gauchat
Part VII. Novel Aspects of Mast Cell Activation and Regulation
Gene Silencing Using Small Interference RNA in Mast Cells
Deling Yin and Charles A. Stuart
Mast Cell Activation by Lipoproteins
Jim Kelley, Gregory Hemontolor, Walid Younis, Chuanfu Li, Guha Krishnaswamy, and David S. Chi
Mast Cell Activation by Stress
Ann L. Baldwin
Part VIII. Roles of Mast Cells in Host Defense
In Vivo Models for Studying Mast Cell-Dependent Responses to Bacterial Infection
Christopher P. Shelburne, James B. McLachlan, and Soman N.
Klappentext
A cutting-edge collection of readily reproducible techniques for the isolation, culture, and study of activation and signaling in human mast cells. These methods take advantage of the latest advances in molecular biology, technology, and information science. They include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis.
Includes supplementary material: sn.pub/extras
