Part I. Introductionn n Introduction to Methods in Molecular Cardiologyn Zhongjie Sunn n Part II. Gene Transfer and Gene Therapy for Cardiovascular Diseasen n Gene Therapy Approaches to Cardiovascular Diseasen J. Mark Jones and Walter J. Kochn n Gene Therapy for Myocardial Ischemia Using the Hypoxia-Inducible Double Plasmid Systemn Yi Tangn n Cardiac Gene Delivery Using DNA-Adenoviral Constructsn J. Mark Jones and Walter J. Kochn n Manipulation of Neuronal Nitric Oxide Synthase Within the Paraventricular Nucleus Using Adenovirus and Antisense Technologyn Yi-Fan Li, Yu Wang, Keith M. Channon, Harold D. Schultz, Irving H. Zucker, and Kaushik P. Pateln n TAT-Mediated Protein Transduction: Delivering Biologically Active Proteins to the Heartn Åsa B. Gustafsson, Roberta A. Gottlieb, and David J. Granvillen n Expression of Human eNOS in Cardiac and Endothelial Cellsn Xiuqing Wang, Robert Cade, and Zhongjie Sunn n Strategies of Conditional Gene Expression in Myocardium: An Overviewn Heather L. Heine, Hon Sing Leong, Fabio M. V. Rossi, Bruce M. McManus, and Thomas J. Podorn n Gene Silencing Using Adenoviral RNAi Vector in Vascular Smooth Muscule Cells and Cardiomyocytesn Hideko Kasahara and Hiroki Aokin n Part III. Stem Cell Therapy for Cardiovascular Diseasen n Cardiac Commitment of Embryonic Stem Cells for Myocardial Repairn Dana Zeineddine, Evangelia Papadimou, Annabelle Mery, Claudine Ménard, and Michel Pucéatn n Autologous Mesenchymal Stem Cells for Post-Ischemic Myocardial Repairn Yao Liang Tangn n Cellular Therapy With Autologous Skeletal Myoblasts for Ischemic Heart Disease and Heart Failuren Yao Liang Tangn n Protocols for Myocardial Infarction Repair Using Fetal Cardiac Myocytesn Sharon Etzion, Radka Holbova, Liron Miller, and Jonathan Leorn n Methods for Examining Stem Cells inPost-Ischemic and Transplanted Heartsn Nana Rezai, Hubert Walinski, Alexandra Kerjner, Lubos Bohunek, Fabio M. V. Rossi, Bruce M. McManus, and Thomas J. Podorn n Endothelial Progenitor Cell Culture and Gene Transfern Hideki Iwaguro and Takayuki Asaharan n Part IV. Gene Analysis in the Injured and Hypertrophied Heartn n Delineation of Sequences Essential for Specific Promoter Activation During Pressure Overloaded Hypertrophy or Factor-Induced Hypertrophyn Chellam Rajamanickam and Radhakrishnan Jeejabai.n n Evaluation of the Cardiac Isoform of a2-Macroglobulin as a Factor Inducing Cardiac Hypertrophyn Chellam Rajamanickam and Radhakrishnan Jeejabain n Detection of Apoptosis in Cardiovascular Diseasesn Arwen L. Hunter, Jonathan C. Choy, and David J. Granvillen n Detection of Cardiac Signaling in the Injured and Hypertrophied Heartn Xiaoning Si, Maziar Rahmani, Ji Yuan, and Honglin Luon n Affymetrix Oligonucleotide Analysis of Gene Expression in the Injured Heartn Bobby Yanagawa, Lydia Taylor, Theresa A. Deisher, Raymond Ng, George F. Schreiner, Timothy J. Triche, Decheng Yang, and Bruce M. McManusn n Identification and Validation of Loci and Candidate Genes Linked to Cardiac Hypertrophyn Bastien Llamas and Christian F. Descheppern n Induction and Analysis of Cardiac Hypertrophy in Transgenic Animal Modelsn Marcos E. Barbosa, Natalia Alenina, and Michael Badern n Methods for the Detection of Altered b-Adrenergic Receptor Signaling Pathways in Hypertrophied Heartsn Matthew J. Wolf, Hideo Tachibana, and Howard A. Rockmann n Part V. Transgenes in Cardiovascular Researchn n Transgenic Mouse Models for Cardiac Dysfunction by a Specific Gene Manipulationn Gopal J. Babu and Muthu Periasamyn n Cardiomyocyte Defects in Diabetic Models and Protection With Cardiac-Targeted Transgenesn Xia Shen, Gang Ye, Naira S. Metreveli, and Pau
The aim of Molecular Cardiology: Methods and Protocols is to document state-of-the-art molecular and genetic techniques in the area of cardiology. These modern approaches enable researchers to readily study heart diseases at the molecular level and will promote the development of new therapeutic str- egies. Methods for genetic dissection, signal transduction, and microarray analysis are excellent tools for the study of the molecular mechanisms of cardiovascular diseases. Protocols for transgenesis take advantage of recent advances in many areas of molecular and cell biology. Transgenic models of heart diseases (cardiac hypertrophy, cardiac dysfunction, and so on. ) are powerful tools for the study of heart disease pathogenesis. Methods for gene transfer to heart tissue using viral and nonviral vectors form the basis of gene therapy for heart diseases. Heart-specific promoters containing a hypox- inducible cardioprotective gene switch are key for protection of the heart from ischemia. Gene and stem cell therapies open novel and exciting avenues for the prevention and treatment of heart diseases. Molecular Cardiology: Methods and Protocols consists of 26 chapters de- ing with various aspects of molecular cardiology, including gene transfer and gene therapy for cardiovascular disease, stem cell therapy for cardiovascular disease, gene analysis in the injured and hypertrophied heart, and transgenesis in cardiovascular research. This book provides step-by-step methods for the successful completion of experimental procedures, and would be useful for both experienced and new investigators in the field of molecular cardiology.
Molecular Cardiology is the first major collection of techniques for gene therapy and stem cell therapy for heart diseases. The methods include readily reproducible techniques for genetic dissection, signal transduction, and microarray analysis. Many of the protocols employ not only molecular and cell biology techniques, but also in vivo approaches to myocardial repair using different types of stem cells, and include methods for expansion, purification, and genetic modification of stem cells.