The aim of Molecular Cardiology: Methods and Protocols is to document state-of-the-art molecular and genetic techniques in the area of cardiology. These modern approaches enable researchers to readily study heart diseases at the molecular level and will promote the development of new therapeutic str- egies. Methods for genetic dissection, signal transduction, and microarray analysis are excellent tools for the study of the molecular mechanisms of cardiovascular diseases. Protocols for transgenesis take advantage of recent advances in many areas of molecular and cell biology. Transgenic models of heart diseases (cardiac hypertrophy, cardiac dysfunction, and so on. ) are powerful tools for the study of heart disease pathogenesis. Methods for gene transfer to heart tissue using viral and nonviral vectors form the basis of gene therapy for heart diseases. Heart-specific promoters containing a hypox- inducible cardioprotective gene switch are key for protection of the heart from ischemia. Gene and stem cell therapies open novel and exciting avenues for the prevention and treatment of heart diseases. Molecular Cardiology: Methods and Protocols consists of 26 chapters de- ing with various aspects of molecular cardiology, including gene transfer and gene therapy for cardiovascular disease, stem cell therapy for cardiovascular disease, gene analysis in the injured and hypertrophied heart, and transgenesis in cardiovascular research. This book provides step-by-step methods for the successful completion of experimental procedures, and would be useful for both experienced and new investigators in the field of molecular cardiology.|Molecular Cardiology is the first major collection of techniques for gene therapy and stem cell therapy for heart diseases. The methods include readily reproducible techniques for genetic dissection, signal transduction, and microarray analysis. Many of the protocols employ not only molecular and cell biology techniques, but also in vivo approaches to myocardial repair using different types of stem cells, and include methods for expansion, purification, and genetic modification of stem cells.
Part I. Introduction Introduction to Methods in Molecular Cardiology Zhongjie Sun Part II. Gene Transfer and Gene Therapy for Cardiovascular Disease Gene Therapy Approaches to Cardiovascular Disease J. Mark Jones and Walter J. Koch Gene Therapy for Myocardial Ischemia Using the Hypoxia-Inducible Double Plasmid System Yi Tang Cardiac Gene Delivery Using DNA-Adenoviral Constructs J. Mark Jones and Walter J. Koch Manipulation of Neuronal Nitric Oxide Synthase Within the Paraventricular Nucleus Using Adenovirus and Antisense Technology Yi-Fan Li, Yu Wang, Keith M. Channon, Harold D. Schultz, Irving H. Zucker, and Kaushik P. Patel TAT-Mediated Protein Transduction: Delivering Biologically Active Proteins to the Heart Åsa B. Gustafsson, Roberta A. Gottlieb, and David J. Granville Expression of Human eNOS in Cardiac and Endothelial Cells Xiuqing Wang, Robert Cade, and Zhongjie Sun Strategies of Conditional Gene Expression in Myocardium: An Overview Heather L. Heine, Hon Sing Leong, Fabio M. V. Rossi, Bruce M. McManus, and Thomas J. Podor Gene Silencing Using Adenoviral RNAi Vector in Vascular Smooth Muscule Cells and Cardiomyocytes Hideko Kasahara and Hiroki Aoki Part III. Stem Cell Therapy for Cardiovascular Disease Cardiac Commitment of Embryonic Stem Cells for Myocardial Repair Dana Zeineddine, Evangelia Papadimou, Annabelle Mery, Claudine Ménard, and Michel Pucéat Autologous Mesenchymal Stem Cells for Post-Ischemic Myocardial Repair Yao Liang Tang Cellular Therapy With Autologous Skeletal Myoblasts for Ischemic Heart Disease and Heart Failure Yao Liang Tang Protocols for Myocardial Infarction Repair Using Fetal Cardiac Myocytes Sharon Etzion, Radka Holbova, Liron Miller, and Jonathan Leor Methods for Examining Stem Cells inPost-Ischemic and Transplanted Hearts Nana Rezai, Hubert Walinski, Alexandra Kerjner, Lubos Bohunek, Fabio M. V. Rossi, Bruce M. McManus, and Thomas J. Podor Endothelial Progenitor Cell Culture and Gene Transfer Hideki Iwaguro and Takayuki Asahara Part IV. Gene Analysis in the Injured and Hypertrophied Heart Delineation of Sequences Essential for Specific Promoter Activation During Pressure Overloaded Hypertrophy or Factor-Induced Hypertrophy Chellam Rajamanickam and Radhakrishnan Jeejabai. Evaluation of the Cardiac Isoform of a2-Macroglobulin as a Factor Inducing Cardiac Hypertrophy Chellam Rajamanickam and Radhakrishnan Jeejabai Detection of Apoptosis in Cardiovascular Diseases Arwen L. Hunter, Jonathan C. Choy, and David J. Granville Detection of Cardiac Signaling in the Injured and Hypertrophied Heart Xiaoning Si, Maziar Rahmani, Ji Yuan, and Honglin Luo Affymetrix Oligonucleotide Analysis of Gene Expression in the Injured Heart Bobby Yanagawa, Lydia Taylor, Theresa A. Deisher, Raymond Ng, George F. Schreiner, Timothy J. Triche, Decheng Yang, and Bruce M. McManus Identification and Validation of Loci and Candidate Genes Linked to Cardiac Hypertrophy Bastien Llamas and Christian F. Deschepper Induction and Analysis of Cardiac Hypertrophy in Transgenic Animal Models Marcos E. Barbosa, Natalia Alenina, and Michael Bader Methods for the Detection of Altered b-Adrenergic Receptor Signaling Pathways in Hypertrophied Hearts Matthew J. Wolf, Hideo Tachibana, and Howard A. Rockman Part V. Transgenes in Cardiovascular Research Transgenic Mouse Models for Cardiac Dysfunction by a Specific Gene Manipulation Gopal J. Babu and Muthu Periasamy Cardiomyocyte Defects in Diabetic Models and Protection With Cardiac-Targeted Transgenes Xia Shen, Gang Ye, Naira S. Metreveli, and Pau
The application of molecular technology to the heart opens a new and promising avenue for both the study and cure of heart diseases that were once thought to be incurable. In Molecular Cardiology: Methods and Protocols, leading cardiac researchers from around the world describe in detail their own proven, cutting-edge techniques for gene therapy and stem cell therapy for heart diseases. The authors provide readily reproducible methods for genetic dissection, signal transduction, and microarray analysis. The protocols include techniques for gene transfer to heart tissue using viral and nonviral vectors, the basis for gene therapy of heart diseases, and for producing transgenic models of heart diseases, the novel and powerful tools for the study of heart disease pathogenesis. Many of the protocols employ not only molecular and cell biology techniques, but also in vivo approaches to myocardial repair using different types of stem cells, and include methods for expansion, purification, and genetic modification of stem cells. The protocols offer step-by-step laboratory instructions, an introduction outlining the principle behind the technique, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, Molecular Cardiology: Methods and Protocols offers basic and clinical researchers the first major collection of the state-of-the-art molecular and cell biology techniques for the study and treatment of genetic and acquired heart diseases.
to Methods in Molecular Cardiology.- Gene Transfer and Gene Therapy for Cardiovascular Disease.- Gene Therapy Approaches to Cardiovascular Disease.- Gene Therapy for Myocardial Ischemia Using the Hypoxia-Inducible Double Plasmid System.- Cardiac Gene Delivery Using DNA-Adenoviral Constructs.- Manipulation of Neuronal Nitric Oxide Synthase Within the Paraventricular Nucleus Using Adenovirus and Antisense Technology.- TAT-Mediated Protein Transduction.- Expression of Human eNOS in Cardiac and Endothelial Cells.- Strategies of Conditional Gene Expression in Myocardium.- Gene Silencing Using Adenoviral RNAi Vector in Vascular Smooth Muscule Cells and Cardiomyocytes.- Stem Cell Therapy for Cardiovascular Disease.- Cardiac Commitment of Embryonic Stem Cells for Myocardial Repair.- Autologous Mesenchymal Stem Cells for Post-Ischemic Myocardial Repair.- Cellular Therapy With Autologous Skeletal Myoblasts for Ischemic Heart Disease and Heart Failure.- Protocols for Myocardial Infarction Repair Using Fetal Cardiac Myocytes.- Methods for Examining Stem Cells in Post-Ischemic and Transplanted Hearts.- Endothelial Progenitor Cell Culture and Gene Transfer.- Gene Analysis in the Injured and Hypertrophied Heart.- Delineation of Sequences Essential for Specific Promoter Activation During Pressure Overloaded Hypertrophy or Factor-Induced Hypertrophy.- Evaluation of the Cardiac Isoform of ?2-Macroglobulin as a Factor Inducing Cardiac Hypertrophy.- Detection of Apoptosis in Cardiovascular Diseases.- Detection of Cardiac Signaling in the Injured and Hypertrophied Heart.- Affymetrix Oligonucleotide Analysis of Gene Expression in the Injured Heart.- Identification and Validation of Loci and Candidate Genes Linked to Cardiac Hypertrophy.- Induction and Analysis of Cardiac Hypertrophy inTransgenic Animal Models.- Methods for the Detection of Altered ?-Adrenergic Receptor Signaling Pathways in Hypertrophied Hearts.- Transgenes in Cardiovascular Research.- Transgenic Mouse Models for Cardiac Dysfunction by a Specific Gene Manipulation.- Cardiomyocyte Defects in Diabetic Models and Protection With Cardiac-Targeted Transgenes.- Cardiac-Specific Overexpression of Angiotensin II Type 1 Receptor in Transgenic Rats.
Inhaltsverzeichnis
Part I. Introduction
Introduction to Methods in Molecular Cardiology
Zhongjie Sun
Part II. Gene Transfer and Gene Therapy for Cardiovascular Disease
Gene Therapy Approaches to Cardiovascular Disease
J. Mark Jones and Walter J. Koch
Gene Therapy for Myocardial Ischemia Using the Hypoxia-Inducible Double Plasmid System
Yi Tang
Cardiac Gene Delivery Using DNA-Adenoviral Constructs
J. Mark Jones and Walter J. Koch
Manipulation of Neuronal Nitric Oxide Synthase Within the Paraventricular Nucleus Using Adenovirus and Antisense Technology
Yi-Fan Li, Yu Wang, Keith M. Channon, Harold D. Schultz, Irving H. Zucker, and Kaushik P. Patel
TAT-Mediated Protein Transduction: Delivering Biologically Active Proteins to the Heart
Åsa B. Gustafsson, Roberta A. Gottlieb, and David J. Granville
Expression of Human eNOS in Cardiac and Endothelial Cells
Xiuqing Wang, Robert Cade, and Zhongjie Sun
Strategies of Conditional Gene Expression in Myocardium: An Overview
Heather L. Heine, Hon Sing Leong, Fabio M. V. Rossi, Bruce M. McManus, and Thomas J. Podor
Gene Silencing Using Adenoviral RNAi Vector in Vascular Smooth Muscule Cells and Cardiomyocytes
Hideko Kasahara and Hiroki Aoki
Part III. Stem Cell Therapy for Cardiovascular Disease
Cardiac Commitment of Embryonic Stem Cells for Myocardial Repair
Dana Zeineddine, Evangelia Papadimou, Annabelle Mery, Claudine Ménard, and Michel Pucéat
Autologous Mesenchymal Stem Cells for Post-Ischemic Myocardial Repair
Yao Liang Tang
Cellular Therapy With Autologous Skeletal Myoblasts for Ischemic Heart Disease and Heart Failure
Yao Liang Tang
Protocols for Myocardial Infarction Repair Using Fetal Cardiac Myocytes
Sharon Etzion, Radka Holbova, Liron Miller, and Jonathan Leor
Methods for Examining Stem Cells inPost-Ischemic and Transplanted Hearts
Nana Rezai, Hubert Walinski, Alexandra Kerjner, Lubos Bohunek, Fabio M. V. Rossi, Bruce M. McManus, and Thomas J. Podor
Endothelial Progenitor Cell Culture and Gene Transfer
Hideki Iwaguro and Takayuki Asahara
Part IV. Gene Analysis in the Injured and Hypertrophied Heart
Delineation of Sequences Essential for Specific Promoter Activation During Pressure Overloaded Hypertrophy or Factor-Induced Hypertrophy
Chellam Rajamanickam and Radhakrishnan Jeejabai.
Evaluation of the Cardiac Isoform of a2-Macroglobulin as a Factor Inducing Cardiac Hypertrophy
Chellam Rajamanickam and Radhakrishnan Jeejabai
Detection of Apoptosis in Cardiovascular Diseases
Arwen L. Hunter, Jonathan C. Choy, and David J. Granville
Detection of Cardiac Signaling in the Injured and Hypertrophied Heart
Xiaoning Si, Maziar Rahmani, Ji Yuan, and Honglin Luo
Affymetrix Oligonucleotide Analysis of Gene Expression in the Injured Heart
Bobby Yanagawa, Lydia Taylor, Theresa A. Deisher, Raymond Ng, George F. Schreiner, Timothy J. Triche, Decheng Yang, and Bruce M. McManus
Identification and Validation of Loci and Candidate Genes Linked to Cardiac Hypertrophy
Bastien Llamas and Christian F. Deschepper
Induction and Analysis of Cardiac Hypertrophy in Transgenic Animal Models
Marcos E. Barbosa, Natalia Alenina, and Michael Bader
Methods for the Detection of Altered b-Adrenergic Receptor Signaling Pathways in Hypertrophied Hearts
Matthew J. Wolf, Hideo Tachibana, and Howard A. Rockman
Part V. Transgenes in Cardiovascular Research
Transgenic Mouse Models for Cardiac Dysfunction by a Specific Gene Manipulation
Gopal J. Babu and Muthu Periasamy
Cardiomyocyte Defects in Diabetic Models and Protection With Cardiac-Targeted Transgenes
Xia Shen, Gang Ye, Naira S. Metreveli, and Pau
Klappentext
The aim of Molecular Cardiology: Methods and Protocols is to document state-of-the-art molecular and genetic techniques in the area of cardiology. These modern approaches enable researchers to readily study heart diseases at the molecular level and will promote the development of new therapeutic str- egies. Methods for genetic dissection, signal transduction, and microarray analysis are excellent tools for the study of the molecular mechanisms of cardiovascular diseases. Protocols for transgenesis take advantage of recent advances in many areas of molecular and cell biology. Transgenic models of heart diseases (cardiac hypertrophy, cardiac dysfunction, and so on. ) are powerful tools for the study of heart disease pathogenesis. Methods for gene transfer to heart tissue using viral and nonviral vectors form the basis of gene therapy for heart diseases. Heart-specific promoters containing a hypox- inducible cardioprotective gene switch are key for protection of the heart from ischemia. Gene and stem cell therapies open novel and exciting avenues for the prevention and treatment of heart diseases. Molecular Cardiology: Methods and Protocols consists of 26 chapters de- ing with various aspects of molecular cardiology, including gene transfer and gene therapy for cardiovascular disease, stem cell therapy for cardiovascular disease, gene analysis in the injured and hypertrophied heart, and transgenesis in cardiovascular research. This book provides step-by-step methods for the successful completion of experimental procedures, and would be useful for both experienced and new investigators in the field of molecular cardiology.
Molecular Cardiology is the first major collection of techniques for gene therapy and stem cell therapy for heart diseases. The methods include readily reproducible techniques for genetic dissection, signal transduction, and microarray analysis. Many of the protocols employ not only molecular and cell biology techniques, but also in vivo approaches to myocardial repair using different types of stem cells, and include methods for expansion, purification, and genetic modification of stem cells.
