Part I. Introductionn n Introduction to Stroke Genomicsn Simon J. Read and Frank C. Baronen n Part II. Preclinical Models and Gene Manipulationn n Choice, Methodology, and Characterization of Focal Ischemic Stroke Models: The Search for Clinical Relevancen David Virleyn n Mutant Animal Models of Stroke and Gene Expression: The Stroke-Prone Spontaneously Hypertensive Ratn Hilary V. O. Carswell, Martin W. McBride, Delyth Graham, Anna F. Dominiczak, and I. Mhairi Macraen n Gene Therapy in Neurological Diseasen Midori A. Yenari and Robert M. Sapolskyn n Stem Cell Transplantation After Middle Cerebral Artery Occlusionn Paul Stroemer and Helen Hodgesn n Endogenous Brain Protection: Models, Gene Expression, and Mechanismsn Frank C. Baronen n Production of Transgenic and Mutant Mouse Modelsn Alex J. Harpern n 3-Nitropropionic Acid Model of Metabolic Stress: Assessment by Magnetic Resonance Imagingn Toby John Robertsn n Part III. Clinical Paradigmsn n Practicalities of Genetic Studies in Human Stroken Ahamad Hassan and Hugh S. Markusn n Evaluation of the Interactions of Common Genetic Mutations in Stroken Zoltán Szolnokin n Part IV. Assessing Differential Expressionn n Technologies of Disease-Related Gene Discovery Using Preclinical Models of Stroken Xinkang Wangn n Quantitative Analysis of Gene Transcription in Stroke Models Using Real-Time RT-PCRn David C. Harrison and Brian C. Bondn n Effective Analysis of Genomic Datan Paul R. Nelson, Andrew B. Goulter, and Richard J. Davisn n Bioinformatic Approaches to Assigning Protein Function From Novel Sequence Datan David Michalovich and Richard Fagann n Pragmatic Target Discovery From Novel Gene to Functionally Defined Drug Target: The Interleukin-1 Storyn Stuart McRae Allann n Indexf
With sequencing of the human genome now complete, deciphering the role of gene function in human neurological pathophysiology is a promise that has yet to be realized. More than most diseases, stroke has been keenly studied from a genomic perspective. Studies are numerous and incorporate data on stroke inheritance, chromosomal loci of risk, preclinical models of stroke, and differential gene expression of brain injury, repair, and recovery. The problem is no longer a lack of information but one of interpretation and prioritization of what we do know. The aims of Stroke Genomics: Methods and Reviews are twofold. First, it aims to provide the reader with cutting-edge reviews of clinical and preclinical genomics, written by leading experts in the field. In particular, the authors of certain chapters relate gene expression changes to physiological end points, such as functional imaging paradigms. Thus, a more holistic approach to gene expression is described, one in which molecular biology goes hand in hand with stroke pathophysiology. Second, detailed methods for study of the molecular biology of stroke are (TM) also included. Following the format of the Methods in Molecular Medicine series, these chapters will enable the reader to employ each technique without recourse to other methods texts. In its entirety, this book should provide the reader with the knowledge needed to design, execute, and interpret preclinical and clinical studies of stroke genomics.
Leading experts explore the pragmatic application of the tools of genomic and molecular biology research to the treatment of stroke. The authors relate changes in gene expression to physiological endpoints-such as functional imaging paradigms-to produce a more holistic approach in which molecular biology goes hand-in-hand with stroke pathophysiology. The book shows how to successfully design, execute, and interpret preclinical and clinical molecular biological studies of stroke as well as rapid development of the next generation of stroke therapeutics.