Part I. Genetic Approachesn n Gene-Targeting and Transgenic Strategies for the Analysis of Hematopoietic Development in the Mousen Hanna K. A. Mikkola and Stuart H. Orkinn n Inducible Transgene Expression in Mouse ES Cellsn David T. Ting, Michael Kyba, and George Q. Daleyn n Quantitative Trait Analysis in the Investigation of Function and Aging in Hematopoietic Stem Cellsn Hans-Willem Snoeckn n Part II. Analysis of Cell Fate Outcomes in Transplantation Modelsn n Flow Cytometric Analysis of Hematopoietic Developmentn Edward F. Srour and Mervin C. Yodern n Reconstitution of Hematopoiesis Following Intrauterine Transplantation of Stem Cellsn Elisabeth H. Javazon, Aziz M. Merchant, Enrico Danzer, and Alan W. Flaken n Reconstitution of Hematopoiesis Following Transplantation Into Neonatal Micen Scott A. Johnson and Mervin C. Yodern n Part III. Animal Modelsn n Drosophilan n Hemocyte Development During Drosophila Embryogenesisn Robert A. Schulz and Nancy Fossettn n Xenopusn n Tracking and Programming Early Hematopoietic Cells in Xenopus Embryosn Maggie Walmsley, Aldo Ciau-Uitz, and Roger Patientn n Fate Mapping Hematopoietic Lineages in the Xenopus Embryon Mary Constance Lane and Michael D. Sheetsn n Analyses of Immune Responses to Ontogeny-Specific Antigens Using an Inbred Strain of Xenopus laevis (J Strain)n Yumi Izutsu and Mitsugu Maénon n Use of Flow Cytometry and Combined DNA/Surface Staining for Analysis of Hematopoietic Development in the Xenopus Embryon James B. Turpenn n Zebrafishn n Analysis of Hematopoietic Development in the Zebrafishn Noëlle N. Paffett-Lugassy and Leonard I. Zonn n Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryosn Philippe Herbomel and Jean-Pierre Levraud. Chickn n In Vivo Methods to Analyze CellOrigins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian Embryon Françoise Dieterlen-Lièvre, Sophie Creuzet, and Josselyne Salaünn n Mousen n Mouse Embryonic Explant Culture System for Analysis of Hematopoietic and Vascular Developmentn Margaret H. Baron and Deanna Mohnn n Hematopoietic Stem Cell Enrichment From the AGM Region of the Mouse Embryon Catherine Robin and Elaine Dzierzakn n Hematopoietic Stem Cell Development During Mouse Embryogenesisn Julien Y. Bertrand, Sebastien Giroux, Ana Cumano, and Isabelle Godinn n Analysis of Hematopoietic Progenitors in the Mouse Embryon James Palis and Anne Koniskin n In Vivo and In Vitro Assays of Thymic Organogenesisn Julie Gordon, Valerie A. Wilson, Billie A. Moore-Scott, Nancy R. Manley, and C. Clare Blackburnn n Retroviral Transduction in Fetal Thymic Organ Culturen Bronwyn M. Owens, Robert G. Hawley, and Lisa M. Spainn n Expansion and Differentiation of Immature Mouse and Human Hematopoietic Progenitorsn Helmut Dolznig, Andrea Kolbus, Cornelia Leberbauer, Uwe Schmidt, Eva-Maria Deiner, Ernst W. Müllner, and Hartmut Beugn n A New Clonal Assay System for Lymphoid and Myeloid Lineagesn Hiroshi Kawamoto and Yoshimoto Katsuran n Hematopoietic and Endothelial Development of Mouse Embryonic Stem Cells in Culturen Kyunghee Choi, Yun Shin Chung, and Weh Jie Zhangn n Analysis of the Vascular Potential of Hematopoietic Stem Cellsn Steven M. Guthrie, Sergio Caballero, Robert N. Mames, Maria B. Grant, and Edward W. Scottn n Dynamic In Vivo Imaging of Mammalian Hematovascular Development Using Whole Embryo Culturen Elizabeth A.V. Jones, Margaret H. Baron, Scott E. Fraser, and Mary E. Dickinsonn n Fluorescent Protein-Cell Labeling and Its Application in Time-Lapse Analysis of Hematopoietic Differentiationn Matthias
During the past few decades, technical and conceptual breakthroughs have led to a virtual revolution in developmental biology. In part through cross-species compa- sons and multidisciplinary approaches (combining, for example, classical embry- ogy, genetics, molecular biology, and systems biology), major questions have often been redefined and examined from new angles and with innovative tools. Analyses using such model systems as Drosophila, Xenopus, zebrafish, chick, human, and mouse have underscored the remarkable extent to which molecular and genetic pa- ways are conserved across species and throughout embryonic, fetal, and adult dev- opment. What we learn from the embryo, then, is not only of fundamental interest, but may well have future practical applications in the clinic. A number of excellent volumes, including several in this series (e. g. , Hema- poietic Stem Cell Protocols, Klug and Jordan, eds. , 2002), have surveyed methods used in the study of hematopoiesis-the processes by which the multiple lineages of the blood form from stem and progenitor cells during ontogeny and throughout the entire life of the animal. These collections of protocols have focused largely on the postnatal cells of mouse and human. Our understanding of hematopoietic devel- ment, however, has benefitted enormously from investigations in a variety of org- isms at different stages of ontogeny.