The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed deillegalscription of how each is performed.
General Strategies Shawn Doonan and Paul Cutler Preparation of Extracts From Animal Tissues J. Mark Skehel Protein Extraction From Plant Tissues Roger J. Fido, E. N. Clare Mills, Neil M. Rigby, and Peter R. Shewry Extraction of Recombinant Protein From Bacteria Anne F. McGettrick and D. Margaret Worrall Protein Extraction From Fungi Paul D. Bridge, Tetsuo Kokubun, and Monique S. J. Simmonds Subcellular Fractionation of Animal Tissues Norma M. Ryan Subcellular Fractionation of Plant Tissues: Isolation of Plastids and Mitochondria Alyson K. Tobin and Caroline G. Bowsher The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds William S. Pierpoint Avoidance of Proteolysis in Extracts Robert J. Beynon and Simon Oliver (Revised by Paul Cutler) Concentration of Extracts Shawn Doonan Making and Changing Buffers Shawn Doonan Purification and Concentration by Ultrafiltration Paul Schratter (Revised by Paul Cutler) Bulk Purification by Fractional Precipitation Shawn Doonan Ion-Exchange Chromatography Chris Selkirk Hydrophobic Interaction Chromatography Paul A. O'Farrell Affinity Chromatography Paul Cutler Dye-Ligand Affinity Chromatography Anne F. McGettrick and D. Margaret Worrall Lectin Affinity Chromatography Iris West and Owen Goldring (Revised by Paul Cutler) Immunoaffinity Chromatography Paul Cutler Immobilized Metal-Ion Affinity Chromatography Tai-Tung Yip and T. William Hutchens (Revised by Paul Cutler) Chromatography on Hydroxyapatite Shawn Doonan Thiophilic Affinity Chromatography and Related Methods Paul Matejtschuk Affinity Precipitation Methods Jane A. Irwin and Keith F. Tipton Isoelectric Focusing Reiner Westermeier Chromatofocusing Timothy J. Mantle and Patricia Noone Size-Exclusion Chromatography Paul Cutler Fast Protein Liquid Chromatography David Sheehan and Siobhan O'Sullivan Reversed-Phase Chromatography of Proteins William A. Neville Extraction of Membrane Proteins Kay Ohlendieck Removal of Detergent From Protein Fractions Kay Ohlendieck Purification of Membrane Proteins Kay Ohlendieck Lyophilization of Proteins Ciarán Ó'Fágáin Storage of Pure Proteins Ciarán Ó'Fágáin Electroelution of Proteins From Polyacrylamide Gels Michael J. Dunn Electroblotting of Proteins From Polyacrylamide Gels Michael J. Dunn Two-Dimensional Polyacrylamide Gel Electrophoresis for Proteome Analyses Neil A. Jones Microscale Solution Isoelectrofocusing: A Sample Prefractionation Method for Comprehensive Proteome Analysis Xun Zuo and David W. Speicher Practical Column Chromatography Shawn Doonan Detection Methods Jacek Mozdzanowski and Sudhir Burman Peptide Proteomics Dean E. McNulty and J. Randall Slemmon Multidimensional Liquid Chromatography of Proteins Rod Watson and Tim Nadler Mass Spectrometry David J. Bell Purification of Therapeutic Proteins Julian Bonnerjea Purification Process Scale-Up Karl Prince and Martin Smith Index
In this new edition of the very successful Protein Purification Protocols (1996), Paul Cutler completely updates the existing protocols to reflect recent advances and adds an enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins. With the many recent advances in technology, simple spectrometric detection is no longer the only option for separating proteins, and the authors treat in full detail all the newer methods for these separations.
Comprehensive and highly practical, Protein Purification Protocols, Second Edition, brings together all the key methodologies that both novice and experienced investigators need to carry out successful experimental work on proteins and their functions today.
From reviews of the first edition...
"...very useful..."
-Angewandte Chemie: International Edition
"...valuable to keep around the laboratory..."
-Biopharm
"...recommended for protein chemists....There is something in this book for
everyone....an excellent working book as well as an excellent reference."
-JAOCS: Journal of the American Oil Chemists' Society
"...an excellent lab companion..."
-SIM News
"...a worthwhile purchase for novice and established practitioners alike."
-Microbiology Today
"...a detailed and comprehensive collection...up to date..invaluable to newcomers and of vast benefit to established researchers/scientists."
-Biomedical Chromatography
"Eight years on this is a welcome new edition which retains the same overall format of a strategies chapter followed by some 43 chapters, each dealing with individual techniques. Updating ranges from new introductions, to additional references and modified protocols. The final six chapters are new and include a useful comparison of detection methods and guides to proteomics and mass spectrometry. The larger format and hard binding make this edition appear less of a bench book, but the Editor has wisely retained the readily approachable format and enhanced its overall value as a practical manual, collating a wide range of protocols useful for both less and more experienced practitioners."-SGM Quarterly
General Strategies.- Preparation of Extracts From Animal Tissues.- Protein Extraction From Plant Tissues.- Extraction of Recombinant Protein From Bacteria.- Protein Extraction From Fungi.- Subcellular Fractionation of Animal Tissues.- Subcellular Fractionation of Plant Tissues.- The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds.- Avoidance of Proteolysis in Extracts.- Concentration of Extracts.- Making and Changing Buffers.- Purification and Concentration by Ultrafiltration.- Bulk Purification by Fractional precipitation.- Ion-Exchange Chromatography.- Hydrophobic Interaction Chromatography.- Affinity Chromatography.- Dye-Ligand Affinity Chromatography.- Lectin Affinity Chromatography.- Immunoaffinity Chromatography.- Immobilized Metal-Ion Affinity Chromatography.- Chromatography on Hydroxyapatite.- Thiophilic Affinity Chromatography and Related Methods.- Affinity Precipitation Methods.- Isoelectric Focusing.- Chromatofocusing.- Size-Exclusion Chromatography.- Fast Protein Liquid Chromatography.- Reversed-Phase Chromatography of Proteins.- Extraction of Membrane Proteins.- Removal of Detergent From Protein Fractions.- Purification of Membrane Proteins.- Lyophilization of Proteins.- Storage of Pure Proteins.- Electroelution of Proteins From Polyacrylamide Gels.- Electroblotting of Proteins From Polyacrylamide Gels.- Two-Dimensional Polyarcylamide Gel Electrophoresis for Proteome Analyses.- Microscale Solution Isoelectrofocusing.- Practical Column Chromatography.- Detection Methods.- Peptide Proteomics.- Multidimensional Liquid Chromatography of Proteins.- Mass Spectrometry.- Purification of Therapeutic Proteins.- Purification Process Scale-Up.
From reviews of the first edition...
"...very useful..."
-Angewandte Chemie: International Edition
"...valuable to keep around the laboratory..."
-Biopharm
"...recommended for protein chemists....There is something in this book for
everyone....an excellent working book as well as an excellent reference."
-JAOCS: Journal of the American Oil Chemists' Society
"...an excellent lab companion..."
-SIM News
"...a worthwhile purchase for novice and established practitioners alike."
-Microbiology Today
"...a detailed and comprehensive collection...up to date..invaluable to newcomers and of vast benefit to established researchers/scientists."
-Biomedical Chromatography
"Eight years on this is a welcome new edition which retains the same overall format of a strategies chapter followed by some 43 chapters, each dealing with individual techniques. Updating ranges from new introductions, to additional references and modified protocols. The final six chapters are new and include a useful comparison of detection methods and guides to proteomics and mass spectrometry. The larger format and hard binding make this edition appear less of a bench book, but the Editor has wisely retained the readily approachable format and enhanced its overall value as a practical manual, collating a wide range of protocols useful for both less and more experienced practitioners."-SGM Quarterly
Inhaltsverzeichnis
General Strategies
Shawn Doonan and Paul Cutler
Preparation of Extracts From Animal Tissues
J. Mark Skehel
Protein Extraction From Plant Tissues
Roger J. Fido, E. N. Clare Mills, Neil M. Rigby, and Peter R. Shewry
Extraction of Recombinant Protein From Bacteria
Anne F. McGettrick and D. Margaret Worrall
Protein Extraction From Fungi
Paul D. Bridge, Tetsuo Kokubun, and Monique S. J. Simmonds
Subcellular Fractionation of Animal Tissues
Norma M. Ryan
Subcellular Fractionation of Plant Tissues: Isolation of Plastids and Mitochondria
Alyson K. Tobin and Caroline G. Bowsher
The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds
William S. Pierpoint
Avoidance of Proteolysis in Extracts
Robert J. Beynon and Simon Oliver (Revised by Paul Cutler)
Concentration of Extracts
Shawn Doonan
Making and Changing Buffers
Shawn Doonan
Purification and Concentration by Ultrafiltration
Paul Schratter (Revised by Paul Cutler)
Bulk Purification by Fractional Precipitation
Shawn Doonan
Ion-Exchange Chromatography
Chris Selkirk
Hydrophobic Interaction Chromatography
Paul A. O'Farrell
Affinity Chromatography
Paul Cutler
Dye-Ligand Affinity Chromatography
Anne F. McGettrick and D. Margaret Worrall
Lectin Affinity Chromatography
Iris West and Owen Goldring (Revised by Paul Cutler)
Immunoaffinity Chromatography
Paul Cutler
Immobilized Metal-Ion Affinity Chromatography
Tai-Tung Yip and T. William Hutchens (Revised by Paul Cutler)
Chromatography on Hydroxyapatite
Shawn Doonan
Thiophilic Affinity Chromatography and Related Methods
Paul Matejtschuk
Affinity Precipitation Methods
Jane A. Irwin and Keith F. Tipton
Isoelectric Focusing
Reiner Westermeier
Chromatofocusing
Timothy J. Mantle and Patricia Noone
Size-Exclusion Chromatography
Paul Cutler
Fast Protein Liquid Chromatography
David Sheehan and Siobhan O'Sullivan
Reversed-Phase Chromatography of Proteins
William A. Neville
Extraction of Membrane Proteins
Kay Ohlendieck
Removal of Detergent From Protein Fractions
Kay Ohlendieck
Purification of Membrane Proteins
Kay Ohlendieck
Lyophilization of Proteins
Ciarán Ó'Fágáin
Storage of Pure Proteins
Ciarán Ó'Fágáin
Electroelution of Proteins From Polyacrylamide Gels
Michael J. Dunn
Electroblotting of Proteins From Polyacrylamide Gels
Michael J. Dunn
Two-Dimensional Polyacrylamide Gel Electrophoresis for Proteome Analyses
Neil A. Jones
Microscale Solution Isoelectrofocusing: A Sample Prefractionation Method for Comprehensive Proteome Analysis
Xun Zuo and David W. Speicher
Practical Column Chromatography
Shawn Doonan
Detection Methods
Jacek Mozdzanowski and Sudhir Burman
Peptide Proteomics
Dean E. McNulty and J. Randall Slemmon
Multidimensional Liquid Chromatography of Proteins
Rod Watson and Tim Nadler
Mass Spectrometry
David J. Bell
Purification of Therapeutic Proteins
Julian Bonnerjea
Purification Process Scale-Up
Karl Prince and Martin Smith
Index
Klappentext
The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed deillegalscription of how each is performed.
Includes supplementary material: sn.pub/extras
