Handbook of Industrial Cell Culture (Englisch)
Mammalian, Microbial, and Plant Cells

The advent of molecular genetics, the progression of transgenic animal and plant methodologies from the laboratory to industrial-scale production, and the development of powerful analytical techniques have each helped standardize the culturing of cells to produce desirable commercial products. In Handbook of Industrial Cell Culture: Mammalian, Microbial, and Plant Cells, a diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotechnological and industrial uses. Special emphasis is given to solving the critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery. The authors all have proven track records in the successful implementation of commercial-scale processes.
Cutting-edge and highly practical, the Handbook of Industrial Cell Culture: Mammalian, Microbial, and Plant Cells not only surveys the best current practices in the art of industrial cell culture across cell types, but also delineates today's evolving technologies with examples of proven practical applications, as well as projections of their future potential. A diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.

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Part I. Mammalian Cell Culture
Analysis and Manipulation of Recombinant Glycoproteins Manufactured in Mammalian Cell Culture
Nigel Jenkins
Genetic Approaches to Recombinant Protein Production in Mammalian Cells
Peter P. Mueller, Dagmar Wirth, Jacqueline Unsinger, and Hansjörg Hauser
Protein Expression Using Transgenic Animals
William H. Velander and Kevin E. van Cott
Mammalian Cell Culture: Process Development Considerations
Steven Rose, Thomas Black, and Divakar Ramakrishnan
Part II. Microbial Cell Culture
Natural Products: Discovery and Screening
Matthew D. Hilton
Genetic Engineering Solutions for Natural Products in Actinomycetes
Richard H. Baltz
Culture Medium Optimization and Scale-Up for Microbial Fermentations
Neal C. Connors
Part III. Plant Cell Culture
Functional Genomics for Plant Trait Discovery
Sam Reddy, Ignacio M. Larrinua, Max O. Ruegger, Vipula K. Shukla, and Yuejin Sun
Molecular Tools for Engineering Plant Cells
Donald J. Merlo
Plant Cell Culture: A Critical Tool for Agricultural Biotechnology
Joseph F. Petolino, Jean L. Roberts, and Ponsamuel Jayakumar
Expression of Recombinant Proteins via the Plastid Genome
Jeffrey M. Staub
Oleosin Partitioning Technology for Production of Recombinant Proteins in Oil Seeds
Maurice M. Moloney
Part IV. Critical Tools for Biotechnology
Genome Sequencing and Genomic Techniques in Drug Discovery and Development
Lawrence M. Gelbert
Proteomics
Gerald W. Becker, Michael D. Knierman, Pavel Shiyanov, and John E. Hale
Metabolic Flux Analysis, Modeling, and Engineering Solutions
Walter M. van Gulik, Wouter A. van Winden, and Joseph J. Heijnen
Advances in Analytical Chemistry for Biotechnology: Mass Spectrometry of Peptides, Proteins, andGlycoproteins
Jeffrey S. Patrick
DNA Shuffling for Whole Cell Engineering
Steve del Cardayre and Keith Powell
Cell Culture Preservation and Storage for Industrial Bioprocesses
James R. Moldenhauer
Index

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Inhaltsverzeichnis

Part I. Mammalian Cell Culture Analysis and Manipulation of Recombinant Glycoproteins Manufactured in Mammalian Cell Culture Nigel Jenkins Genetic Approaches to Recombinant Protein Production in Mammalian Cells Peter P. Mueller, Dagmar Wirth, Jacqueline Unsinger, and Hansjörg Hauser Protein Expression Using Transgenic Animals William H. Velander and Kevin E. van Cott Mammalian Cell Culture: Process Development Considerations Steven Rose, Thomas Black, and Divakar Ramakrishnan Part II. Microbial Cell Culture Natural Products: Discovery and Screening Matthew D. Hilton Genetic Engineering Solutions for Natural Products in Actinomycetes Richard H. Baltz Culture Medium Optimization and Scale-Up for Microbial Fermentations Neal C. Connors Part III. Plant Cell Culture Functional Genomics for Plant Trait Discovery Sam Reddy, Ignacio M. Larrinua, Max O. Ruegger, Vipula K. Shukla, and Yuejin Sun Molecular Tools for Engineering Plant Cells Donald J. Merlo Plant Cell Culture: A Critical Tool for Agricultural Biotechnology Joseph F. Petolino, Jean L. Roberts, and Ponsamuel Jayakumar Expression of Recombinant Proteins via the Plastid Genome Jeffrey M. Staub Oleosin Partitioning Technology for Production of Recombinant Proteins in Oil Seeds Maurice M. Moloney Part IV. Critical Tools for Biotechnology Genome Sequencing and Genomic Techniques in Drug Discovery and Development Lawrence M. Gelbert Proteomics Gerald W. Becker, Michael D. Knierman, Pavel Shiyanov, and John E. Hale Metabolic Flux Analysis, Modeling, and Engineering Solutions Walter M. van Gulik, Wouter A. van Winden, and Joseph J. Heijnen Advances in Analytical Chemistry for Biotechnology: Mass Spectrometry of Peptides, Proteins, andGlycoproteins Jeffrey S. Patrick DNA Shuffling for Whole Cell Engineering Steve del Cardayre and Keith Powell Cell Culture Preservation and Storage for Industrial Bioprocesses James R. Moldenhauer Index

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Klappentext

A diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.