5. Matrix-Assisted Laser-Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF/MS)-Based Identification of Pathogens from Positive Blood Culture Bottles

Philippe Lagacé-Wiens

6. Bacterial and Fungal DNA Extraction from Positive Blood Culture Bottles: A Manual and an Automated Protocol

Minna Mäki

7. Broad-Range-PCR in the Identification of Bacterial and Fungal Pathogens from Positive Blood Culture Bottles: A Sequencing Approach

Yoshitomo Morinaga and Katsunori Yanagihara

8. Identification of Bacterial and Fungal Pathogens from Positive Blood Culture Bottles: A Microarray-Based Approach

Teresa Raich and Scott Powell

9. Detection of Carbapenemases Using Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Meropenem Hydrolysis Assay

Jroslav Hrabák

10. Molecular Detection of Antibiotic Resistance Genes from Positive Blood Cultures

Musa Y. Hindiyeh, Gill Smollan, Shiraz Gefen-Halevi, Ella Mendelson, and Nathan Keller

11. Bacterial and Fungal DNA Extraction from Blood Samples: Manual Protocols

Michael G. Lorenz, Helge Mühl, and Claudia Disqué

12. Bacterial and Fungal DNA Extraction from Blood Samples: Automated Protocols

Michael G. Lorenz, Claudia Disqué, and Helge Mühl

13. Broad-Range-PCR in the Identification of Bacterial and Fungal Pathogens from Blood Samples: A Sequencing Approach

Eva Leitner and Harald H. Kessler

14. Real-Time PCR-Based Identification of Bacterial and Fungal Pathogens from Blood Samples

Madeleine Mai, Iris Müller, Daniela Maneg, Achim Haecker, Gerd Haberhausen, and Klaus-Peter Hunfeld

15. Host Response Biomarkers in the Diagnosis of Sepsis: A General Overview

Marianna Parlato and Jean-Marc Cavaillon

16. Host Response Biomarkers in Sepsis: The Role of Procalcitonin

Jean-Louis Vincent, Marc Van Nuffelen, and Christophe Lelubre

17. Host Response Biomarkers in Sepsis: Overview on sTREM-1 Detection

Jérémie Lemarié, Damien Barraud, and Sébastien Gibot

18. Host Response Biomarkers in Sepsis: suPAR Detection

Evangelos J. Giamarellos-Bourboulis and Marianna Georgitsi

19. Clinical Diagnosis of Sepsis and the Combined Use of Biomarkers, Culture- and Non-Culture-Based Assays

Frank Bloos

Klappentext

This volume covers microbiological, clinical and patophysiological aspects of sepsis and also provides general overview chapters with every chapter discussing the real clinical impact of the discussed diagnostic approaches. Sepsis is a major clinical problem that takes an inordinate toll on human lives and economical resources. It is widely recognized that inappropriate treatment is associated with a dramatic increase in mortality, especially within the first hours, therefore clinical and microbiological diagnosis are of pivotal importance in the management of septic patients. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Sepsis: Diagnostic Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this life-threatening illness.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts