This volume covers microbiological, clinical and patophysiological aspects of sepsis and also provides general overview chapters with every chapter discussing the real clinical impact of the discussed diagnostic approaches. Sepsis is a major clinical problem that takes an inordinate toll on human lives and economical resources. It is widely recognized that inappropriate treatment is associated with a dramatic increase in mortality, especially within the first hours, therefore clinical and microbiological diagnosis are of pivotal importance in the management of septic patients. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Sepsis: Diagnostic Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this life-threatening illness.
1. Microbiological Diagnosis of Sepsis: The Confounding Effect of a "Gold Standard"
Nicasio Mancini, Roberto Burioni, and Massimo Clementi
2. Pathopysiological Aspects of Sepsis: An Overview
Ying-Yi Luan, Yong-Ming Yao, Qing-Hong Zhang, and Zhi-Yong Sheng
3. Clinical Aspects of Sepsis: An Overview
Giacomo Monti, Giovanni Landoni, Daiana Taddei, Francesca Isella, and Alberto Zangrillo
4. Technical Improvements in Culturing Blood
Giacomo Pardini
5. Matrix-Assisted Laser-Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF/MS)-Based Identification of Pathogens from Positive Blood Culture Bottles
Philippe Lagacé-Wiens
6. Bacterial and Fungal DNA Extraction from Positive Blood Culture Bottles: A Manual and an Automated Protocol
Minna Mäki
7. Broad-Range-PCR in the Identification of Bacterial and Fungal Pathogens from Positive Blood Culture Bottles: A Sequencing Approach
Yoshitomo Morinaga and Katsunori Yanagihara
8. Identification of Bacterial and Fungal Pathogens from Positive Blood Culture Bottles: A Microarray-Based Approach
Teresa Raich and Scott Powell
9. Detection of Carbapenemases Using Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Meropenem Hydrolysis Assay
Jroslav Hrabák
10. Molecular Detection of Antibiotic Resistance Genes from Positive Blood Cultures
Musa Y. Hindiyeh, Gill Smollan, Shiraz Gefen-Halevi, Ella Mendelson, and Nathan Keller
11. Bacterial and Fungal DNA Extraction from Blood Samples: Manual Protocols
Michael G. Lorenz, Helge Mühl, and Claudia Disqué
12. Bacterial and Fungal DNA Extraction from Blood Samples: Automated Protocols
Michael G. Lorenz, Claudia Disqué, and Helge Mühl
13. Broad-Range-PCR in the Identification of Bacterial and Fungal Pathogens from Blood Samples: A Sequencing Approach
Eva Leitner and Harald H. Kessler
14. Real-Time PCR-Based Identification of Bacterial and Fungal Pathogens from Blood Samples
Madeleine Mai, Iris Müller, Daniela Maneg, Achim Haecker, Gerd Haberhausen, and Klaus-Peter Hunfeld
15. Host Response Biomarkers in the Diagnosis of Sepsis: A General Overview
Marianna Parlato and Jean-Marc Cavaillon
16. Host Response Biomarkers in Sepsis: The Role of Procalcitonin
Jean-Louis Vincent, Marc Van Nuffelen, and Christophe Lelubre
17. Host Response Biomarkers in Sepsis: Overview on sTREM-1 Detection
Jérémie Lemarié, Damien Barraud, and Sébastien Gibot
18. Host Response Biomarkers in Sepsis: suPAR Detection
Evangelos J. Giamarellos-Bourboulis and Marianna Georgitsi
19. Clinical Diagnosis of Sepsis and the Combined Use of Biomarkers, Culture- and Non-Culture-Based Assays
Frank Bloos
1
Inhaltsverzeichnis
1. Microbiological Diagnosis of Sepsis: The Confounding Effect of a "Gold Standard"
Nicasio Mancini, Roberto Burioni, and Massimo Clementi
2. Pathopysiological Aspects of Sepsis: An Overview
Ying-Yi Luan, Yong-Ming Yao, Qing-Hong Zhang, and Zhi-Yong Sheng
3. Clinical Aspects of Sepsis: An Overview
Giacomo Monti, Giovanni Landoni, Daiana Taddei, Francesca Isella, and Alberto Zangrillo
4. Technical Improvements in Culturing Blood
Giacomo Pardini
5. Matrix-Assisted Laser-Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF/MS)-Based Identification of Pathogens from Positive Blood Culture Bottles
Philippe Lagacé-Wiens
6. Bacterial and Fungal DNA Extraction from Positive Blood Culture Bottles: A Manual and an Automated Protocol
Minna Mäki
7. Broad-Range-PCR in the Identification of Bacterial and Fungal Pathogens from Positive Blood Culture Bottles: A Sequencing Approach
Yoshitomo Morinaga and Katsunori Yanagihara
8. Identification of Bacterial and Fungal Pathogens from Positive Blood Culture Bottles: A Microarray-Based Approach
Teresa Raich and Scott Powell
9. Detection of Carbapenemases Using Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Meropenem Hydrolysis Assay
Jroslav Hrabák
10. Molecular Detection of Antibiotic Resistance Genes from Positive Blood Cultures
Musa Y. Hindiyeh, Gill Smollan, Shiraz Gefen-Halevi, Ella Mendelson, and Nathan Keller
11. Bacterial and Fungal DNA Extraction from Blood Samples: Manual Protocols
Michael G. Lorenz, Helge Mühl, and Claudia Disqué
12. Bacterial and Fungal DNA Extraction from Blood Samples: Automated Protocols
Michael G. Lorenz, Claudia Disqué, and Helge Mühl
13. Broad-Range-PCR in the Identification of Bacterial and Fungal Pathogens from Blood Samples: A Sequencing Approach
Eva Leitner and Harald H. Kessler
14. Real-Time PCR-Based Identification of Bacterial and Fungal Pathogens from Blood Samples
Madeleine Mai, Iris Müller, Daniela Maneg, Achim Haecker, Gerd Haberhausen, and Klaus-Peter Hunfeld
15. Host Response Biomarkers in the Diagnosis of Sepsis: A General Overview
Marianna Parlato and Jean-Marc Cavaillon
16. Host Response Biomarkers in Sepsis: The Role of Procalcitonin
Jean-Louis Vincent, Marc Van Nuffelen, and Christophe Lelubre
17. Host Response Biomarkers in Sepsis: Overview on sTREM-1 Detection
Jérémie Lemarié, Damien Barraud, and Sébastien Gibot
18. Host Response Biomarkers in Sepsis: suPAR Detection
Evangelos J. Giamarellos-Bourboulis and Marianna Georgitsi
19. Clinical Diagnosis of Sepsis and the Combined Use of Biomarkers, Culture- and Non-Culture-Based Assays
Frank Bloos
Klappentext
This volume covers microbiological, clinical and patophysiological aspects of sepsis and also provides general overview chapters with every chapter discussing the real clinical impact of the discussed diagnostic approaches. Sepsis is a major clinical problem that takes an inordinate toll on human lives and economical resources. It is widely recognized that inappropriate treatment is associated with a dramatic increase in mortality, especially within the first hours, therefore clinical and microbiological diagnosis are of pivotal importance in the management of septic patients. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Sepsis: Diagnostic Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this life-threatening illness.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
