Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2 , a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1 st and 2 nd editions, these two volumes offer Agrobacterium -mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included.
Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula , and Setaria viridis . It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.
Part I
Vectors And Model Plants
1
Gateway ® Compatible Plant Transformation Vectors
Mark A. Smedley and Wendy A. Harwood
2
Brachypodium Distachyon
Jennifer N. Bragg, Amy Anderton, Rita Nieu, And John P. Vogel
3
Medicago Truncatula Transformation Using Cotyledonary Explants
Elane Wright and Zeng-Yu Wang
4
Medicago Truncatula Transformation Using Leaf Explants
Viviane Cosson, Alexis Eschstruth, and Pascal Ratet
5
Setaria Viridis
Joyce Van Eck And Kerry Swartwood
Part II
Cereal Plants
6
Barley (Hordeum Vulgare L.) Transformation Using Immature Embryos
Cornelia Marthe, Jochen Kumlehn and Goetz Hensel
7
Barley (Hordeum Vulgare L.) Transformation Using Embryogenic Pollen Cultures
Ingrid Otto, Andrea Müller, and Jochen Kumlehn
8
Maize (Zea Mays L.)
Bronwyn Frame, Katey Warnberg, Marcy Main, and Kan Wang
9
Maize, Tropical (Zea Mays L.)
Shireen K. Assem
10
Finger Millet [Eleusine Coracana (L.) Gaertn]
Stanislaus Antony Ceasar, and Savarimuthu Ignacimuthu
11
Oat (Avena Sativa L.)
Sebastian Gasparis and Anna Nadolska-Orczyk
12
Rice, Indica (Oryza Sativa L.)
Yukoh Hiei, Yuji Ishida and Toshihiko Komari
13
Rice, Japonica (Oryza Sativa L.)
Marcy Main, Bronwyn Frame And Kan Wang
14
Sorghum (Sorghum Bicolor L.)
Yuechun Wan And Jeanne Layton
15
Wheat (Triticum Aestivum L.) Transformation Using Immature Embryos
Yuji Ishida, Masako Tsunashima, Yukoh Hiei And Toshihiko Komari
16
Wheat (Triticum Aestivum L.) Transformation Using Mature Embryos
Eva Medvecká And Wendy A Harwood
Part III
Legume Plants
17
Alfalfa (Medicago Sativa L.)
Chunxiang Fu, Timothy Hernandez, Chuanen Zhou, and Zeng-Yu Wang
18
Clovers (Trifolium Spp.)
Samira Rahimi-Ashtiani, Sareena Sahab, Stephen Panter, John Mason, and German Spangenberg
19
Clover, Red (Trifolium Pratense)
Michael L. Sullivan and Kenneth H. Quesenberry
20
Cowpea [Vigna Unguiculata (L.) Walp]
Ratikanta Behura, Sanjeev Kumar, Bedabrata Saha, Manas Kumar Panda, Mohitosh Dey, Ayan Sadhukhan, Sagarika Mishra, Shamsher Alam, Debee Prasad Sahoo, Twinkle Sugla, Sanjib Kumar Panda, and Lingaraj Sahoo
22
Lentil (Lens Culinaris Medik.)
Ufuk Celikkol Akcay, Meral Yücel, Hüseyin Avni Öktem
22
Soybean [Glycine Max (L.) Merr.]
Diane Luth, Katey Warnburg, and Kan Wang
Part IV
Legume Plants
23
Brassica Oleracea And B. Napus
Penny A.C. Hundleby Nee Sparrow, and Judith A. Irwin
24
Cucumber (Cucumis Sativus L.) and Kabocha Squash (Cucurbita Moschata Duch)
Yoshihiko Nanasato and Yutaka Tabei
25
Pepper, Chili (Capsicum Annuum)
Jung Min, Sun Hee Shin, En Mi Jeon, Jung Mi Park, Ji Young Hyun,and Chee Hark Harn
26
Pepper, Sweet (Capsicum Annuum)
Iris Heidmann And Kim Boutilier
27
Sugar Beet (Beta Vulgaris L.)
Hiroyo Kagami, Masayuki Kurata, Hiroaki Matsuhira, Kazunori Taguchi, Tetsuo Mikami, Hideto Tamagake, and Tomohiko Kubo
28
Tomato (Solanum Lycopersicum)
Martha L. Orozco-Cárdenas, Dora Garcia, and Javier Narváez-Vásquez
1
Inhaltsverzeichnis
Part I
Vectors And Model Plants
1
Gateway® Compatible Plant Transformation Vectors
Mark A. Smedley and Wendy A. Harwood
2
Brachypodium Distachyon
Jennifer N. Bragg, Amy Anderton, Rita Nieu, And John P. Vogel
3
Medicago Truncatula Transformation Using Cotyledonary Explants
Elane Wright and Zeng-Yu Wang
4
Medicago Truncatula Transformation Using Leaf Explants
Viviane Cosson, Alexis Eschstruth, and Pascal Ratet
5
Setaria Viridis
Joyce Van Eck And Kerry Swartwood
Part II
Cereal Plants
6
Barley (Hordeum Vulgare L.) Transformation Using Immature Embryos
Cornelia Marthe, Jochen Kumlehn and Goetz Hensel
7
Barley (Hordeum Vulgare L.) Transformation Using Embryogenic Pollen Cultures
Ingrid Otto, Andrea Müller, and Jochen Kumlehn
8
Maize (Zea Mays L.)
Bronwyn Frame, Katey Warnberg, Marcy Main, and Kan Wang
9
Maize, Tropical (Zea Mays L.)
Shireen K. Assem
10
Finger Millet [Eleusine Coracana (L.) Gaertn]
Stanislaus Antony Ceasar, and Savarimuthu Ignacimuthu
11
Oat (Avena Sativa L.)
Sebastian Gasparis and Anna Nadolska-Orczyk
12
Rice, Indica (Oryza Sativa L.)
Yukoh Hiei, Yuji Ishida and Toshihiko Komari
13
Rice, Japonica (Oryza Sativa L.)
Marcy Main, Bronwyn Frame And Kan Wang
14
Sorghum (Sorghum Bicolor L.)
Yuechun Wan And Jeanne Layton
15
Wheat (Triticum Aestivum L.) Transformation Using Immature Embryos
Yuji Ishida, Masako Tsunashima, Yukoh Hiei And Toshihiko Komari
16
Wheat (Triticum Aestivum L.) Transformation Using Mature Embryos
Eva Medvecká And Wendy A Harwood
Part III
Legume Plants
17
Alfalfa (Medicago Sativa L.)
Chunxiang Fu, Timothy Hernandez, Chuanen Zhou, and Zeng-Yu Wang
18
Clovers (Trifolium Spp.)
Samira Rahimi-Ashtiani, Sareena Sahab, Stephen Panter, John Mason, and German Spangenberg
19
Clover, Red (Trifolium Pratense)
Michael L. Sullivan and Kenneth H. Quesenberry
20
Cowpea [Vigna Unguiculata (L.) Walp]
Ratikanta Behura, Sanjeev Kumar, Bedabrata Saha, Manas Kumar Panda, Mohitosh Dey, Ayan Sadhukhan, Sagarika Mishra, Shamsher Alam, Debee Prasad Sahoo, Twinkle Sugla, Sanjib Kumar Panda, and Lingaraj Sahoo
22
Lentil (Lens Culinaris Medik.)
Ufuk Celikkol Akcay, Meral Yücel, Hüseyin Avni Öktem
22
Soybean [Glycine Max (L.) Merr.]
Diane Luth, Katey Warnburg, and Kan Wang
Part IV
Legume Plants
23
Brassica Oleracea And B. Napus
Penny A.C. Hundleby Nee Sparrow, and Judith A. Irwin
24
Cucumber (Cucumis Sativus L.) and Kabocha Squash (Cucurbita Moschata Duch)
Yoshihiko Nanasato and Yutaka Tabei
25
Pepper, Chili (Capsicum Annuum)
Jung Min, Sun Hee Shin, En Mi Jeon, Jung Mi Park, Ji Young Hyun,and Chee Hark Harn
26
Pepper, Sweet (Capsicum Annuum)
Iris Heidmann And Kim Boutilier
27
Sugar Beet (Beta Vulgaris L.)
Hiroyo Kagami, Masayuki Kurata, Hiroaki Matsuhira, Kazunori Taguchi, Tetsuo Mikami, Hideto Tamagake, and Tomohiko Kubo
28
Tomato (Solanum Lycopersicum)
Martha L. Orozco-Cárdenas, Dora Garcia, and Javier Narváez-Vásquez
Klappentext
Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included.
Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis. It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.
Emphasizes agricultural crops or plant species with economic values
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
