Includes cutting-edge methods and protocols with a focus on TNF Superfamily members in health and disease
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Exploring these type II trans-membrane proteins, The TNF Superfamily: Methods and Protocols focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Opening with protocols to understand the signaling process of TNF family members, this detailed volume continues with technical examples of investigating the role of TNF family members in physiopathologies, protocols on modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, as well as methodologies for various assays of TNF family members and the production of recombinant molecules. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Practical and ready to use, The TNF Superfamily: Methods and Protocols will aid researchers investigating this key family of proteins, involved in vital processes such as providing signals for activation, differentiation, survival and death of cells, modulation of immune response and inflammation, hematopoiesis and osteoclastogenesis.
1. Assaying NF-kB Activation and Signaling from TNF Receptors
Zhenzhen Wu and Leslie A. Bruggeman
2. Dissecting DR3 Signaling
Yelena L. Pobezinskaya and Zhenggang Liu
3. Modulation of FceRI-Dependent Mast Cell Response by OX40L
Riccardo Sibilano, Carlo Pucillo, and Barbara Frossi
4. Analyzing the Signaling Capabilities of Soluble and Membrane TWEAK
Johannes Trebing, José Antonio Carmona Arana, Steffen Salzmann, and Harald Wajant
5. Regulation of Human Dendritic Cell Functions by Natural Anti-CD40 Antibodies
Sri Ramulu Elluru, Srini V Kaveri, and Jagadeesh Bayry
6. ?-actin in the Signaling of Transmembrane TNF-?-Mediated Cytotoxicity
Hui Chen, Yan Leng, and Zhuoya Li
7. Investigating the Protective Role of Death Receptor 3 (DR3) in Renal Injury Using an Organ Culture Model
Rafia S. Al-Lamki
8. Analysis of TNF-Mediated Cerebral Pericyte Remodeling
Jennifer V. Welser-Alves, Amin Boroujerdi, Ulrich Tigges, and Richard Milner
9. CD137 in Chronic Graft-Versus-Host Disease
Juyang Kim, Hogng R. Cho, and Byungsuk Kwon
10. In Vitro Investigation of the Roles of the Proinflammatory Cytokines Tumor Necrosis Factor-? and Interleukin-1 in Murine Osteoclastogenesis
Joel Jules and Xu Feng
11. Evasion of TNF-? Mediated Apoptosis by Hepatitis C Virus
Hangeun Kim and Ranjit Ray
12. TNF-? Modulates TLR2-Dependent Responses During Mycobacterial Infection
Sahana Holla, Jamma Trinath, and Kithiganahalli Narayanaswamy Balaji
13. Experimental Applications of TNF-Reporter Mice with Far-Red Fluorescent Label
Yury V. Shebzukhov, Anna A. Kuchmiy, Andrei A. Kruglov, Frauke Zipp, Volker Siffrin, and Sergei A. Nedospasov
14. A Simple Solid-Phase Assay for Studying Direct Binding of Progranulin to TNFR and Progranulin Antagonism of TNF/TNFR Interactions
Tian Qingyun, Shuai Zhao, and Chuanju Liu
15. Compartment-Specific Flow Cytometry for the Analysis of TNF-Mediated Recruitment and Activation of Glomerular Leukocytes in Murine Kidneys
Volker Vielhauer
16. Determination of Soluble Tumor Necrosis Factor Receptor 2 Produced by Alternative Splicing
Xavier Romero, Juan D. Cañete, and Pablo Engel
17. Effective Expression and Purification of Bioactive Recombinant Soluble LIGHT
Isamu Tsuji, Keiji Iwamoto, and Yasushi Shintani
18. One of the TNF Superfamily Members: Bi-Functional Protein, TNFR2-Fc-IL-1ra
Bo Xie
Exploring these type II trans-membrane proteins, The TNF Superfamily: Methods and Protocols focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Opening with protocols to understand the signaling process of TNF family members, this detailed volume continues with technical examples of investigating the role of TNF family members in physiopathologies, protocols on modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, as well as methodologies for various assays of TNF family members and the production of recombinant molecules. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and ready to use,
The TNF Superfamily: Methods and Protocols will aid researchers investigating this key family of proteins, involved in vital processes such as providing signals for activation, differentiation, survival and death of cells, modulation of immune response and inflammation, hematopoiesis, and osteoclastogenesis.
Exploring these type II trans-membrane proteins, The TNF Superfamily: Methods and Protocols focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Opening with protocols to understand the signaling process of TNF family members, this detailed volume continues with technical examples of investigating the role of TNF family members in physiopathologies, protocols on modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, as well as methodologies for various assays of TNF family members and the production of recombinant molecules. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Practical and ready to use, The TNF Superfamily: Methods and Protocols will aid researchers investigating this key family of proteins, involved in vital processes such as providing signals for activation, differentiation, survival and death of cells, modulation of immune response and inflammation, hematopoiesis and osteoclastogenesis.
1. Assaying NF-kB Activation and Signaling from TNF Receptors
Zhenzhen Wu and Leslie A. Bruggeman
2. Dissecting DR3 Signaling
Yelena L. Pobezinskaya and Zhenggang Liu
3. Modulation of FceRI-Dependent Mast Cell Response by OX40L
Riccardo Sibilano, Carlo Pucillo, and Barbara Frossi
4. Analyzing the Signaling Capabilities of Soluble and Membrane TWEAK
Johannes Trebing, José Antonio Carmona Arana, Steffen Salzmann, and Harald Wajant
5. Regulation of Human Dendritic Cell Functions by Natural Anti-CD40 Antibodies
Sri Ramulu Elluru, Srini V Kaveri, and Jagadeesh Bayry
6. beta-actin in the Signaling of Transmembrane TNF- -Mediated Cytotoxicity
Hui Chen, Yan Leng, and Zhuoya Li
7. Investigating the Protective Role of Death Receptor 3 (DR3) in Renal Injury Using an Organ Culture Model
Rafia S. Al-Lamki
8. Analysis of TNF-Mediated Cerebral Pericyte Remodeling
Jennifer V. Welser-Alves, Amin Boroujerdi, Ulrich Tigges, and Richard Milner
9. CD137 in Chronic Graft-Versus-Host Disease
Juyang Kim, Hogng R. Cho, and Byungsuk Kwon
10. In Vitro Investigation of the Roles of the Proinflammatory Cytokines Tumor Necrosis Factor- and Interleukin-1 in Murine Osteoclastogenesis
Joel Jules and Xu Feng
11. Evasion of TNF- Mediated Apoptosis by Hepatitis C Virus
Hangeun Kim and Ranjit Ray
12. TNF- Modulates TLR2-Dependent Responses During Mycobacterial Infection
Sahana Holla, Jamma Trinath, and Kithiganahalli Narayanaswamy Balaji
13. Experimental Applications of TNF-Reporter Mice with Far-Red Fluorescent Label
Yury V. Shebzukhov, Anna A. Kuchmiy, Andrei A. Kruglov, Frauke Zipp, Volker Siffrin, and Sergei A. Nedospasov
14. A Simple Solid-Phase Assay for Studying Direct Binding of Progranulin to TNFR and Progranulin Antagonism of TNF/TNFR Interactions
Tian Qingyun, Shuai Zhao, and Chuanju Liu
15. Compartment-Specific Flow Cytometry for the Analysis of TNF-Mediated Recruitment and Activation of Glomerular Leukocytes in Murine Kidneys
Volker Vielhauer
16. Determination of Soluble Tumor Necrosis Factor Receptor 2 Produced by Alternative Splicing
Xavier Romero, Juan D. Cañete, and Pablo Engel
17. Effective Expression and Purification of Bioactive Recombinant Soluble LIGHT
Isamu Tsuji, Keiji Iwamoto, and Yasushi Shintani
18. One of the TNF Superfamily Members: Bi-Functional Protein, TNFR2-Fc-IL-1ra
Bo Xie
Inhaltsverzeichnis
1. Assaying NF-kB Activation and Signaling from TNF Receptors
Zhenzhen Wu and Leslie A. Bruggeman
2. Dissecting DR3 Signaling
Yelena L. Pobezinskaya and Zhenggang Liu
3. Modulation of FceRI-Dependent Mast Cell Response by OX40L
Riccardo Sibilano, Carlo Pucillo, and Barbara Frossi
4. Analyzing the Signaling Capabilities of Soluble and Membrane TWEAK
Johannes Trebing, José Antonio Carmona Arana, Steffen Salzmann, and Harald Wajant
5. Regulation of Human Dendritic Cell Functions by Natural Anti-CD40 Antibodies
Sri Ramulu Elluru, Srini V Kaveri, and Jagadeesh Bayry
6. ß-actin in the Signaling of Transmembrane TNF-a-Mediated Cytotoxicity
Hui Chen, Yan Leng, and Zhuoya Li
7. Investigating the Protective Role of Death Receptor 3 (DR3) in Renal Injury Using an Organ Culture Model
Rafia S. Al-Lamki
8. Analysis of TNF-Mediated Cerebral Pericyte Remodeling
Jennifer V. Welser-Alves, Amin Boroujerdi, Ulrich Tigges, and Richard Milner
9. CD137 in Chronic Graft-Versus-Host Disease
Juyang Kim, Hogng R. Cho, and Byungsuk Kwon
10. In Vitro Investigation of the Roles of the Proinflammatory Cytokines Tumor Necrosis Factor-a and Interleukin-1 in Murine Osteoclastogenesis
Joel Jules and Xu Feng
11. Evasion of TNF-a Mediated Apoptosis by Hepatitis C Virus
Hangeun Kim and Ranjit Ray
12. TNF-a Modulates TLR2-Dependent Responses During Mycobacterial Infection
Sahana Holla, Jamma Trinath, and Kithiganahalli Narayanaswamy Balaji
13. Experimental Applications of TNF-Reporter Mice with Far-Red Fluorescent Label
Yury V. Shebzukhov, Anna A. Kuchmiy, Andrei A. Kruglov, Frauke Zipp, Volker Siffrin, and Sergei A. Nedospasov
14. A Simple Solid-Phase Assay for Studying Direct Binding of Progranulin to TNFR and Progranulin Antagonism of TNF/TNFR Interactions
Tian Qingyun, Shuai Zhao, and Chuanju Liu
15. Compartment-Specific Flow Cytometry for the Analysis of TNF-Mediated Recruitment and Activation of Glomerular Leukocytes in Murine Kidneys
Volker Vielhauer
16. Determination of Soluble Tumor Necrosis Factor Receptor 2 Produced by Alternative Splicing
Xavier Romero, Juan D. Cañete, and Pablo Engel
17. Effective Expression and Purification of Bioactive Recombinant Soluble LIGHT
Isamu Tsuji, Keiji Iwamoto, and Yasushi Shintani
18. One of the TNF Superfamily Members: Bi-Functional Protein, TNFR2-Fc-IL-1ra
Bo Xie
Klappentext
Exploring these type II trans-membrane proteins, The TNF Superfamily: Methods and Protocols focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Opening with protocols to understand the signaling process of TNF family members, this detailed volume continues with technical examples of investigating the role of TNF family members in physiopathologies, protocols on modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, as well as methodologies for various assays of TNF family members and the production of recombinant molecules. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.nPractical and ready to use, The TNF Superfamily: Methods and Protocols will aid researchers investigating this key family of proteins, involved in vital processes such as providing signals for activation, differentiation, survival and death of cells, modulation of immune response and inflammation, hematopoiesis and osteoclastogenesis.
Includes cutting-edge methods and protocols with a focus on TNF Superfamily members in health and disease
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts