Includes cutting-edge methods and protocols to support the creation of future safe and efficacious cancer vaccines
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes cutting-edge methods and protocols to support the creation of future safe and efficacious cancer vaccines
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Cancer Vaccines: Methods and Protocols explores the manipulation and modification of immune cells, the manipulation and modification of tumor cells as well as the manipulation of immune/tumor interactions and various delivery mechanisms, with the overall end goal of evoking a tumor-specific response and overcoming the immuno-evasive mechanisms employed by the tumor cells. This detailed volume also covers the subject of cancer vaccines in a more global sense with its section on the advances, challenges, and future of cancer vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Cancer Vaccines: Methods and Protocols aims to help guide researchers toward developing further generations of cancer vaccines that are both safe and efficacious, with the hope that cancer vaccines will be the standard of care in the very near future.
Part I: Manipulation and Modification of Immune Cells: Dendritic Cells
1. Single Step Antigen Loading and Maturation of Dendritic Cells Through mRNA Electroporation of a Tumor-Associated Antigen and a TriMix of Costimulatory Molecules
Daphné Benteyn, An M.T. Van Nuffel, Sofie Wilgenhof, and Aude Bonehill
2. Generation of Multiple Peptide Cocktail-Pulsed Dendritic Cells as a Cancer Vaccine
Hyun-Ju Lee, Nu-Ri Choi, Manh-Cuong Vo, My-Dung Hoang, Youn-Kyung Lee, and Je-Jung Lee
3. Pulsing Dendritic Cells with Whole Tumor Cell Lysates
Laura Alaniz, Manglio M. Rizzo, and Guillermo Mazzolini
4. Antigen Trapping by Dendritic Cells for Anti-Tumor Therapy
Chiranjib Pal
5. Ex Vivo Loading of Autologous Dendritic Cells with Tumor Antigens
Manglio M. Rizzo, Laura Alaniz, and Guillermo Mazzolini
6. Tumor Antigen/Cytokine-Pulsed Dendritic Cells in Therapy Against Lymphoma
Sumit K. Hira, Deepak Verma, and Partha P. Manna
7. Dendritic Cells Primed with Protein-Protein Fusion Adjuvant
Liying Wang and Yongli Yu
8. Antigen-Specific mRNA Transfection of Autologous Dendritic Cells
Fabian Benencia
9. Electroporation of Dendritic Cells with Autologous Total RNA from Tumor Material
Francesca Milano and K.K. Krishnadath
10. Dendritic Cells Transfected with Adenoviral Vectors as Vaccines
Joseph Senesac, Dmitry Gabrilovich, Samuel Pirruccello, and James E. Talmadge
11. Genetic Modification of Dendritic Cells with RNAi
Xiao-Tong Song
12. Fast Monocyte-Derived Dendritic Cells-Based Immunotherapy
Gamal Ramadan
13. Intratumoral Injection of BCG-CWS Pretreated Dendritic Cells Following Tumor Cryoablation
Naoshi Kawamura, Masaru Udagawa, Tomonobu Fujita, Toshiharu Sakurai, Tomonori Yaguchi, and Yutaka Kawakami
14. Exploiting the CD1d-iNKT Cell Axis for Potentiation of DC-Based Cancer Vaccines
Roeland Lameris, Famke L. Schneiders, Tanja D. de Gruijl, and Hans J. van der Vliet
Part II: Manipulation and Modification of Immune Cells: T Lymphocytes and NK Cells
15. Modification of T Lymphocytes to Express Tumor Antigens
Aaron E. Foster and Xiao-Tong Song
16. Genetic Modification of Mouse Effector and Helper T Lymphocytes Expressing a Chimeric Antigen Receptor
Liza B. John, Tess M. Chee, David E. Gilham, and Phillip K. Darcy
17. Genetic Modification of Cytotoxic T Lymphocytes to Express Cytokine Receptors
Serena K. Perna, Barbara Savoldo, and Gianpietro Dotti
18. Monitoring the Frequency and Function of Regulatory T Cells and Summary of the Approaches Currently Used to Inhibit Regulatory T Cells in Cancer Patients
Chiara Camisaschi, Marcella Tazzari, Licia Rivoltini, and Chiara Castelli
19. Cytokine Activation of Natural Killer Cells
Syh-Jae Lin, Pei-Tzu Lee, and Ming-Ling Kuo
Part III: Manipulation and Modification of Tumor Cells
20. Loading of Acute Myeloid Leukemia Cells with Poly(I:C) by Electroporation
Eva Lion, Charlotte M. de Winde, Viggo F.I. Van Tendeloo, and Evelien L.J.M. Smits
21. Autologous Tumor Cells Engineered to Express Bacterial Antigens
Vijayakumar K. Ramiya, Maya M. Jerald, Patricia D. Lawman, and Michael J.P. Lawman
22. Tumor Cell Transformation Using Antisense Oligonucleotide
Mohamed R. Akl and Nehad M. Ayoub
23. The Direct Display of Costimulatory Proteins on Tumor Cells as a Means of Vaccination for Cancer Immunotherapy
Haval Shirwan, Esma S. Yolcu, Rajesh K. Sharma, Hong Zaho, and Orlando Grimany-Nuno
Part IV: Manipulation of Immune/Tumor Interactions
24. Cloning Variable Region Genes of Clonal Lymphoma Immunoglobulin for Generating Patient-Specific Idiotype DNA Vaccine
Soung-chul Cha, Hong Qin, Ippei Sakamaki, and Larry Kwak
25. Heat Shock Proteins Purified from Autologous Tumors Using Antibody-Based Affinity Chromatography
Christian Kleist, Marco Randazzo, Janina Jiga, and Peter Terness
26. Invariant Chain-Peptide Fusion Vaccine Using HER-2/neu
Sonia A. Perez, George E. Peoples, Michael Papamichail, and Constantin N. Baxevanis
27. TLR-9 Agonist Immunostimulatory Sequence Adjuvants Linked to Cancer Antigens
Hidekazu Shirota and Dennis M. Klinman
28. Production of Multiple CTL Epitopes from Multiple Tumor-Associated Antigens
Rena Morita, Yoshihiko Hirohashi, Munehide Nakatsugawa, Takayuki Kanaseki, Toshihiko Torigoe, and Noriyuki Sato
29. Preparation of Polypeptides Comprising Multiple TAA Peptides
Bing Ni, Zhengcai Jia, and Yuzhang Wu
30. Idiotype Vaccine Production Using Hybridoma Technology
Susana Inoges, Ascension Lopez-Diaz de Cerio, Helena Villanueva, Fernando Pastor, and Maurizio Bendandi
31. Preparation of Cancer-Related Peptide Cocktails that Target Heterogeneously-Expressed Antigens
Reshu Gupta and Pradip P Sachdeva
Part V: Delivery Mechanisms
32. Making an Avipoxvirus Encoding a Tumor-Associated Antigen and a Costimulatory Molecule
Paul M. Howley, Kerrilyn R. Diener, and John D. Hayball
33. Bacterial Vectors for the Delivery of Tumor Antigens
Yan Wang, Bertrand Toussaint, and Audrey Le Gouëllec
34. Preparation of Peptide Microspheres Using Tumor Antigen-Derived Peptides
Santwana Bhatnagar, Raza Ali Naqvi, Riyasat Ali, and D.N. Rao
35. Production of Antigen-Loaded Biodegradable Nanoparticles and Uptake by Dendritic Cells
Vijaya Bharti Joshi, Sean M. Geary, and Aliasger K. Salem
36. Development of Plasmid-Lipid Complexes for Direct Intratumoral Injection
Rama P. Kotipatruni and Ganji Purnachandra Nagaraju
Part VI: The Advances, Challenges, and Future of Cancer Vaccines
37. The Use of Dendritic Cells for Peptide-Based Vaccination in Cancer Immunotherapy
Mohamed L. Salem
38. Advances in Host and Vector Development for the Production of Plasmid DNA Vaccines
Juergen Mairhofer and Alvaro R. Lara
39. Challenges Facing the Development of Cancer Vaccines
Mayer Fishman
40. Future of Cancer Vaccines
Hauke Winter, Bernard A. Fox, and Dominik Rüttinger
Cancer Vaccines: Methods and Protocols explores the manipulation and modification of immune cells, the manipulation and modification of tumor cells, as well as the manipulation of immune/tumor interactions and various delivery mechanisms, with the overall end goal of evoking a tumor-specific response and overcoming the immuno-evasive mechanisms employed by the tumor cells. This detailed volume also covers the subject of cancer vaccines in a more global sense with its section on the advances, challenges, and future of cancer vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative,
Cancer Vaccines: Methods and Protocols aims to help guide researchers toward developing further generations of cancer vaccines that are both safe and efficacious, with the hope that cancer vaccines will be the standard of care in the very near future.
From the reviews:
"This book describes cancer vaccine protocols targeting cancers that have already developed, stimulating the immune system against the tumor to stop cancer cell growth and reduce tumor burden. ... The audience includes established researchers, professors, and healthcare professionals who have a basic understanding of general and tumor immunology. ... This is a well-written, well-structured book that covers a rapidly developing and highly important field of vaccine cancer research.” (K. K. Wong, Doody´s Book Reviews, June, 2014)
Cancer Vaccines: Methods and Protocols explores the manipulation and modification of immune cells, the manipulation and modification of tumor cells as well as the manipulation of immune/tumor interactions and various delivery mechanisms, with the overall end goal of evoking a tumor-specific response and overcoming the immuno-evasive mechanisms employed by the tumor cells. This detailed volume also covers the subject of cancer vaccines in a more global sense with its section on the advances, challenges, and future of cancer vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Cancer Vaccines: Methods and Protocols aims to help guide researchers toward developing further generations of cancer vaccines that are both safe and efficacious, with the hope that cancer vaccines will be the standard of care in the very near future.
Single Step Antigen Loading and Maturation of Dendritic Cells Through mRNA Electroporation of a Tumor-Associated Antigen and a TriMix of Costimulatory Molecules.- Generation of Multiple Peptide Cocktail-Pulsed Dendritic Cells as a Cancer Vaccine.- Pulsing Dendritic Cells with Whole Tumor Cell Lysates.- Antigen Trapping by Dendritic Cells for Anti-Tumor Therapy.- Ex Vivo Loading of Autologous Dendritic Cells with Tumor Antigens.- Tumor Antigen/Cytokine-Pulsed Dendritic Cells in Therapy Against Lymphoma.- Dendritic Cells Primed with Protein-Protein Fusion Adjuvant.- Antigen-Specific mRNA Transfection of Autologous Dendritic Cells.- Electroporation of Dendritic Cells with Autologous Total RNA from Tumor Material.- Dendritic Cells Transfected with Adenoviral Vectors as Vaccines.- Genetic Modification of Dendritic Cells with RNAi.- Fast Monocyte-Derived Dendritic Cells-Based Immunotherapy.- Intratumoral Injection of BCG-CWS Pretreated Dendritic Cells Following Tumor Cryoablation.- Exploiting the CD1d-iNKT Cell Axis for Potentiation of DC-Based Cancer Vaccines.- Modification of T Lymphocytes to Express Tumor Antigens.- Genetic Modification of Mouse Effector and Helper T Lymphocytes Expressing a Chimeric Antigen Receptor.- Genetic Modification of Cytotoxic T Lymphocytes to Express Cytokine Receptors.- Monitoring the Frequency and Function of Regulatory T Cells and Summary of the Approaches Currently Used to Inhibit Regulatory T Cells in Cancer Patients.- Cytokine Activation of Natural Killer Cells.- Loading of Acute Myeloid Leukemia Cells with Poly(I:C) by Electroporation.- Autologous Tumor Cells Engineered to Express Bacterial Antigens.- Tumor Cell Transformation Using Antisense Oligonucleotide.- The Direct Display of Costimulatory Proteins on Tumor Cells as a Means of Vaccination for Cancer Immunotherapy.- Cloning Variable Region Genes of Clonal Lymphoma Immunoglobulin for Generating Patient-Specific Idiotype DNA Vaccine.- Heat Shock Proteins Purified from Autologous Tumors Using Antibody-Based Affinity Chromatography.- Invariant Chain-Peptide Fusion Vaccine Using HER-2/neu.- TLR-9 Agonist Immunostimulatory Sequence Adjuvants Linked to Cancer Antigens.- Production of Multiple CTL Epitopes from Multiple Tumor-Associated Antigens.- Preparation of Polypeptides Comprising Multiple TAA Peptides.- Idiotype Vaccine Production Using Hybridoma Technology.- Preparation of Cancer-Related Peptide Cocktails that Target Heterogeneously-Expressed Antigens.- Making an Avipoxvirus Encoding a Tumor-Associated Antigen and a Costimulatory Molecule.- Bacterial Vectors for the Delivery of Tumor Antigens.- Preparation of Peptide Microspheres Using Tumor Antigen-Derived Peptides.- Production of Antigen-Loaded Biodegradable Nanoparticles and Uptake by Dendritic Cells.- Development of Plasmid-Lipid Complexes for Direct Intratumoral Injection.- The Use of Dendritic Cells for Peptide-Based Vaccination in Cancer Immunotherapy.- Advances in Host and Vector Development for the Production of Plasmid DNA Vaccines.- Challenges Facing the Development of Cancer Vaccines.- Future of Cancer Vaccines.
From the reviews:
"This book describes cancer vaccine protocols targeting cancers that have already developed, stimulating the immune system against the tumor to stop cancer cell growth and reduce tumor burden. ... The audience includes established researchers, professors, and healthcare professionals who have a basic understanding of general and tumor immunology. ... This is a well-written, well-structured book that covers a rapidly developing and highly important field of vaccine cancer research." (K. K. Wong, Doody's Book Reviews, June, 2014)
Inhaltsverzeichnis
Part I: Manipulation and Modification of Immune Cells: Dendritic Cells
1. Single Step Antigen Loading and Maturation of Dendritic Cells Through mRNA Electroporation of a Tumor-Associated Antigen and a TriMix of Costimulatory Molecules
Daphné Benteyn, An M.T. Van Nuffel, Sofie Wilgenhof, and Aude Bonehill
2. Generation of Multiple Peptide Cocktail-Pulsed Dendritic Cells as a Cancer Vaccine
Hyun-Ju Lee, Nu-Ri Choi, Manh-Cuong Vo, My-Dung Hoang, Youn-Kyung Lee, and Je-Jung Lee
3. Pulsing Dendritic Cells with Whole Tumor Cell Lysates
Laura Alaniz, Manglio M. Rizzo, and Guillermo Mazzolini
4. Antigen Trapping by Dendritic Cells for Anti-Tumor Therapy
Chiranjib Pal
5. Ex Vivo Loading of Autologous Dendritic Cells with Tumor Antigens
Manglio M. Rizzo, Laura Alaniz, and Guillermo Mazzolini
6. Tumor Antigen/Cytokine-Pulsed Dendritic Cells in Therapy Against Lymphoma
Sumit K. Hira, Deepak Verma, and Partha P. Manna
7. Dendritic Cells Primed with Protein-Protein Fusion Adjuvant
Liying Wang and Yongli Yu
8. Antigen-Specific mRNA Transfection of Autologous Dendritic Cells
Fabian Benencia
9. Electroporation of Dendritic Cells with Autologous Total RNA from Tumor Material
Francesca Milano and K.K. Krishnadath
10. Dendritic Cells Transfected with Adenoviral Vectors as Vaccines
Joseph Senesac, Dmitry Gabrilovich, Samuel Pirruccello, and James E. Talmadge
11. Genetic Modification of Dendritic Cells with RNAi
Xiao-Tong Song
12. Fast Monocyte-Derived Dendritic Cells-Based Immunotherapy
Gamal Ramadan
13. Intratumoral Injection of BCG-CWS Pretreated Dendritic Cells Following Tumor Cryoablation
Naoshi Kawamura, Masaru Udagawa, Tomonobu Fujita, Toshiharu Sakurai, Tomonori Yaguchi, and Yutaka Kawakami
14. Exploiting the CD1d-iNKT Cell Axis for Potentiation of DC-Based Cancer Vaccines
Roeland Lameris, Famke L. Schneiders, Tanja D. de Gruijl, and Hans J. van der Vliet
Part II: Manipulation and Modification of Immune Cells: T Lymphocytes and NK Cells
15. Modification of T Lymphocytes to Express Tumor Antigens
Aaron E. Foster and Xiao-Tong Song
16. Genetic Modification of Mouse Effector and Helper T Lymphocytes Expressing a Chimeric Antigen Receptor
Liza B. John, Tess M. Chee, David E. Gilham, and Phillip K. Darcy
17. Genetic Modification of Cytotoxic T Lymphocytes to Express Cytokine Receptors
Serena K. Perna, Barbara Savoldo, and Gianpietro Dotti
18. Monitoring the Frequency and Function of Regulatory T Cells and Summary of the Approaches Currently Used to Inhibit Regulatory T Cells in Cancer Patients
Chiara Camisaschi, Marcella Tazzari, Licia Rivoltini, and Chiara Castelli
19. Cytokine Activation of Natural Killer Cells
Syh-Jae Lin, Pei-Tzu Lee, and Ming-Ling Kuo
Part III: Manipulation and Modification of Tumor Cells
20. Loading of Acute Myeloid Leukemia Cells with Poly(I:C) by Electroporation
Eva Lion, Charlotte M. de Winde, Viggo F.I. Van Tendeloo, and Evelien L.J.M. Smits
21. Autologous Tumor Cells Engineered to Express Bacterial Antigens
Vijayakumar K. Ramiya, Maya M. Jerald, Patricia D. Lawman, and Michael J.P. Lawman
22. Tumor Cell Transformation Using Antisense Oligonucleotide
Mohamed R. Akl and Nehad M. Ayoub
23. The Direct Display of Costimulatory Proteins on Tumor Cells as a Means of Vaccination for Cancer Immunotherapy
Haval Shirwan, Esma S. Yolcu, Rajesh K. Sharma, Hong Zaho, and Orlando Grimany-Nuno
Part IV: Manipulation of Immune/Tumor Interactions
24. Cloning Variable Region Genes of Clonal Lymphoma Immunoglobulin for Generating Patient-Specific Idiotype DNA Vaccine
Soung-chul Cha, Hong Qin, Ippei Sakamaki, and Larry Kwak
25. Heat Shock Proteins Purified from Autologous Tumors Using Antibody-Based Affinity Chromatography
Christian Kleist, Marco Randazzo, Janina Jiga, and Peter Terness
26. Invariant Chain-Peptide Fusion Vaccine Using HER-2/neu
Sonia A. Perez, George E. Peoples, Michael Papamichail, and Constantin N. Baxevanis
27. TLR-9 Agonist Immunostimulatory Sequence Adjuvants Linked to Cancer Antigens
Hidekazu Shirota and Dennis M. Klinman
28. Production of Multiple CTL Epitopes from Multiple Tumor-Associated Antigens
Rena Morita, Yoshihiko Hirohashi, Munehide Nakatsugawa, Takayuki Kanaseki, Toshihiko Torigoe, and Noriyuki Sato
29. Preparation of Polypeptides Comprising Multiple TAA Peptides
Bing Ni, Zhengcai Jia, and Yuzhang Wu
30. Idiotype Vaccine Production Using Hybridoma Technology
Susana Inoges, Ascension Lopez-Diaz de Cerio, Helena Villanueva, Fernando Pastor, and Maurizio Bendandi
31. Preparation of Cancer-Related Peptide Cocktails that Target Heterogeneously-Expressed Antigens
Reshu Gupta and Pradip P Sachdeva
Part V: Delivery Mechanisms
32. Making an Avipoxvirus Encoding a Tumor-Associated Antigen and a Costimulatory Molecule
Paul M. Howley, Kerrilyn R. Diener, and John D. Hayball
33. Bacterial Vectors for the Delivery of Tumor Antigens
Yan Wang, Bertrand Toussaint, and Audrey Le Gouëllec
34. Preparation of Peptide Microspheres Using Tumor Antigen-Derived Peptides
Santwana Bhatnagar, Raza Ali Naqvi, Riyasat Ali, and D.N. Rao
35. Production of Antigen-Loaded Biodegradable Nanoparticles and Uptake by Dendritic Cells
Vijaya Bharti Joshi, Sean M. Geary, and Aliasger K. Salem
36. Development of Plasmid-Lipid Complexes for Direct Intratumoral Injection
Rama P. Kotipatruni and Ganji Purnachandra Nagaraju
Part VI: The Advances, Challenges, and Future of Cancer Vaccines
37. The Use of Dendritic Cells for Peptide-Based Vaccination in Cancer Immunotherapy
Mohamed L. Salem
38. Advances in Host and Vector Development for the Production of Plasmid DNA Vaccines
Juergen Mairhofer and Alvaro R. Lara
39. Challenges Facing the Development of Cancer Vaccines
Mayer Fishman
40. Future of Cancer Vaccines
Hauke Winter, Bernard A. Fox, and Dominik Rüttinger
Klappentext
Cancer Vaccines: Methods and Protocols explores the manipulation and modification of immune cells, the manipulation and modification of tumor cells as well as the manipulation of immune/tumor interactions and various delivery mechanisms, with the overall end goal of evoking a tumor-specific response and overcoming the immuno-evasive mechanisms employed by the tumor cells. This detailed volume also covers the subject of cancer vaccines in a more global sense with its section on the advances, challenges, and future of cancer vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.nComprehensive and authoritative, Cancer Vaccines: Methods and Protocols aims to help guide researchers toward developing further generations of cancer vaccines that are both safe and efficacious, with the hope that cancer vaccines will be the standard of care in the very near future.
Includes cutting-edge methods and protocols to support the creation of future safe and efficacious cancer vaccines
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
