Part I. Introduction. An Introduction to Plant Cell Culture: Pointers to Success, R. D. Hall. Part II. Cell Culture and Plant Regeneration. Callus Initiation, Maintenance, and Shoot Induction in Rice, N. W. Blackhall, J. P. Jotham, K. Azakanandam, J. B. Power, K. C. Lowe, E. C. Cocking, and M. R. Davey. Callus Initiation, Maintenance, and Shoot Induction in Potato, R. F. Curry and A. C. Cassells. Somatic Embryogenesis in Barley Suspension Cultures, M. Kihara, H. Funatsuki, K. Ito, and P. A. Lazzeri. Somatic Embryogenesis in Picea Suspension Cultures, U. Egertsdotter. Specialized Techniques. Direct, Cyclic Somatic Embryogenesis in Cassava for Mass Production Purposes, K. J. J. M. Raemakers, E. Jacobsen, and R. G. F. Visser. Immature Inflorescence Culture of Cereals, S. Rasco-Gaunt and P. Barcelo. Cryopreservation of Rice Tissue Cultures, E. E. Benson and P. T. Lynch. Noncryogenic Long-Term Germplasm Storage, A. Golmirzaie and J. Toledo. Part III. Plant Propagation In Vitro. Microprogation of Strawberry via Axillary Shoot Proliferation, P. Boxus. Meristem-Tip Culture for Propagation and Virus Elimination, B. W. W. Grout. Specialized Techniques. Clonal Propagation of Orchids, B. Tisserat and D. Jones. In Vitro Propagation of Succulent Plants, J. Gratton and M. F. Fay. Microproragation of Flower Bulbs: Lily and Narcissus, M. M. Langens-Gerrits and G-J . M. de Klerk. Clonal Propagation of Woody Species, I. S. Harry and T. A. Thorpe. Spore-Derived Axenic Cultures of Ferns as a Method of Propagation, M. V. Ford and M. F. Fay. Part IV. Applications for Plant Protoplasts. Protoplast Isolation, Culture, and Plant Regeneration from Passiflora Leaves, P. Anthony, W. Otoni, J. B. Power, K. C. Lowe, and M. R. Davey. Isolation, Culture, and Plant Regeneration from Suspension-Derived Protoplasts of Lolium, M. Folling and A. Olesen. Protoplast Fusion for Symmetric Somatic Hybrid Production in Brassicaceae, J. Fahleson and K. Glimelius. Production of Cybrids in Rapeseed (Brassica napus), S. Yarrow. Specialized Techniques. Microprotoplast-Mediated Chromosome Transfer (MMCT) for the Direct Production of Monosomic Addition Lines, K. S. Ramulu, P. Dijkhuis, F. A. Krens, and H. A. Verhoeven. Guard Cell Protoplasts: Isolation, Culture, and Regeneration of Plants, G. Boorse and G. Tallman. In Vitro Fertilization with Isolated, Single Gametes, E. Kranz. Part V. Protocols for Genomic Manipulation. Protocols for Anther and Microspore Culture of Barley, A. Jähne-Gärtner and H. Lorz . Microspore Embryogenesis and In Vitro Pollen Maturation in Tobacco, A. Touraev and E. Heberle-Bors. Embryo Rescue Following Wide Crosses, H. C. Sharma. Specialized Techniques. Mutagenesis and the Selection of Resistant Mutants, P. J. Dix. The Generation of Plastid Mutants In Vitro, P. J. Dix. Part VI. Protocols for the Introduction of Specific Genes. Agrobacterium-Mediated Transformation of Petunia Leaf Discs, I. M. van der Meer. Transformation of Rice Via PEG-Mediated DNA Uptake into Protoplasts, K. Datta and S. K. Datta. Transformation of Wheat Via Particle Bombardment, I. K. Vasil and V. Vasil. Plant Transformation Via Protoplast Electroporation, G. W. Bates. Specialized Techniques. Transformation of Maize Via Tissue Electroporation, K. D'Halluin, E. Bonne, M. Bossut, and R. Le Page. Transformation of Maize Using Silicon Carbide Whiskers, J. M. Dunwell. Part VII. Suspension Culture Initiation and the Accumulation of Metabolites. Directing Anthraquinone Accumulation Via Manipulation of Morinda Suspension Cultures, M. J. M. Hagendoorn, D. C. L. Jamar, and L. H. W. van der Plas. Alkaloid Accumulation in Catharanthus Roseus Suspension Cultures, A. H. Scragg. Betalains: Their Accumulation and Release In Vitro, C.S. Hunter and N. J. Kilby. Appendix. Index.
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