Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods cover culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants. Plant Cell Culture Protocols's state-of-the-art techniques are certain to make the book today's reference of choice, an indispensable tool in the development of new transgenic plants and full-scale commercial applications.
Part I. Introduction. An Introduction to Plant Cell Culture: Pointers to Success, R. D. Hall. Part II. Cell Culture and Plant Regeneration. Callus Initiation, Maintenance, and Shoot Induction in Rice, N. W. Blackhall, J. P. Jotham, K. Azakanandam, J. B. Power, K. C. Lowe, E. C. Cocking, and M. R. Davey. Callus Initiation, Maintenance, and Shoot Induction in Potato, R. F. Curry and A. C. Cassells. Somatic Embryogenesis in Barley Suspension Cultures, M. Kihara, H. Funatsuki, K. Ito, and P. A. Lazzeri. Somatic Embryogenesis in Picea Suspension Cultures, U. Egertsdotter. Specialized Techniques. Direct, Cyclic Somatic Embryogenesis in Cassava for Mass Production Purposes, K. J. J. M. Raemakers, E. Jacobsen, and R. G. F. Visser. Immature Inflorescence Culture of Cereals, S. Rasco-Gaunt and P. Barcelo. Cryopreservation of Rice Tissue Cultures, E. E. Benson and P. T. Lynch. Noncryogenic Long-Term Germplasm Storage, A. Golmirzaie and J. Toledo. Part III. Plant Propagation In Vitro. Microprogation of Strawberry via Axillary Shoot Proliferation, P. Boxus. Meristem-Tip Culture for Propagation and Virus Elimination, B. W. W. Grout. Specialized Techniques. Clonal Propagation of Orchids, B. Tisserat and D. Jones. In Vitro Propagation of Succulent Plants, J. Gratton and M. F. Fay. Microproragation of Flower Bulbs: Lily and Narcissus, M. M. Langens-Gerrits and G-J . M. de Klerk. Clonal Propagation of Woody Species, I. S. Harry and T. A. Thorpe. Spore-Derived Axenic Cultures of Ferns as a Method of Propagation, M. V. Ford and M. F. Fay. Part IV. Applications for Plant Protoplasts. Protoplast Isolation, Culture, and Plant Regeneration from Passiflora Leaves, P. Anthony, W. Otoni, J. B. Power, K. C. Lowe, and M. R. Davey. Isolation, Culture, and Plant Regeneration from Suspension-Derived Protoplasts of Lolium, M. Folling and A. Olesen. Protoplast Fusion for Symmetric Somatic Hybrid Production in Brassicaceae, J. Fahleson and K. Glimelius. Production of Cybrids in Rapeseed (Brassica napus), S. Yarrow. Specialized Techniques. Microprotoplast-Mediated Chromosome Transfer (MMCT) for the Direct Production of Monosomic Addition Lines, K. S. Ramulu, P. Dijkhuis, F. A. Krens, and H. A. Verhoeven. Guard Cell Protoplasts: Isolation, Culture, and Regeneration of Plants, G. Boorse and G. Tallman. In Vitro Fertilization with Isolated, Single Gametes, E. Kranz. Part V. Protocols for Genomic Manipulation. Protocols for Anther and Microspore Culture of Barley, A. Jähne-Gärtner and H. Lorz . Microspore Embryogenesis and In Vitro Pollen Maturation in Tobacco, A. Touraev and E. Heberle-Bors. Embryo Rescue Following Wide Crosses, H. C. Sharma. Specialized Techniques. Mutagenesis and the Selection of Resistant Mutants, P. J. Dix. The Generation of Plastid Mutants In Vitro, P. J. Dix. Part VI. Protocols for the Introduction of Specific Genes. Agrobacterium-Mediated Transformation of Petunia Leaf Discs, I. M. van der Meer. Transformation of Rice Via PEG-Mediated DNA Uptake into Protoplasts, K. Datta and S. K. Datta. Transformation of Wheat Via Particle Bombardment, I. K. Vasil and V. Vasil. Plant Transformation Via Protoplast Electroporation, G. W. Bates. Specialized Techniques. Transformation of Maize Via Tissue Electroporation, K. D'Halluin, E. Bonne, M. Bossut, and R. Le Page. Transformation of Maize Using Silicon Carbide Whiskers, J. M. Dunwell. Part VII. Suspension Culture Initiation and the Accumulation of Metabolites. Directing Anthraquinone Accumulation Via Manipulation of Morinda Suspension Cultures, M. J. M. Hagendoorn, D. C. L. Jamar, and L. H. W. van der Plas. Alkaloid Accumulation in Catharanthus Roseus Suspension Cultures, A. H. Scragg. Betalains: Their Accumulation and Release In Vitro, C.S. Hunter and N. J. Kilby. Appendix. Index.
". . . a valuable update on the technology, with emphasis on methods for micropropagation and gene transfer. . .the editor has assembled an excellent range of expert authors and the volume is well balanced and up-to-date. . .thoroughly recommended."-Plant Growth Regulation
"Protocols are presented for many agronomically important plants including the culture and regeneration of rice, potato, barley, casava, spruce, Pasiflora, Lolium, and various species of Brassica. The organization of all of the chapters is consistent including an introduction, a materials section divided into appropriate subsections, a similarly subdivided methods section, a notes section that contains important observations to make or precautions to take, and a final reference section. . .the techniques are well written and include specific examples and step-by-step methods with appropriate references. . .will make an excellent addition to the library of researchers who wish to extend their collection of basic plant cell culture techniques."-Quarterly Review of Biology
"The combination of many years of experience with new experimental entries is often very successful and of high quality...represents one of the highest quality manuals on this topic published with the last ten years...it will assure the relevance of an important scientific discipline, plant biotechnology, in today's world."-Biologia Plantarum
"The book brings together a series of well written, step-by-step protocols on the most frequently used techniques in plant cell and tissue culture, covering various aspects of culture initiation, maintenance, long-term storage and manipulation....In addition to protocols on widely applied methods, each part includes representative protocols for more specialized techniques....All the chapters are well structured, profusely illustrated with original photographs and line drawings, and contain detailed descriptions of materials, culture media and comprehensively described experimental procedures. The reader will certainly appreciate the precautionary notes and additional hints on each technique and each protocol provided at the end of each chapter."-Elsevier
"This book presents a comprehensive collection of applicable techniques for plant cell and tissue culture. The methods cover culture initiation, maintenance, manipulation, application and long-term storage, with emphasis on techniques for genetic modification and micropropagation." - Lavoisier Librairie
Springer Book Archives