New Experimental Approaches.- Patch Pipettes Used for Loading Small Cells with Flourescent Indicator Dyes.- Optical Detection of Calcium-Dependent ATP Release From Stimulated Medullary Chromaffin Cells.- Glucose-evoked Changes in [K+] and [Ca2+] in the Intercellular Spaces of the Mouse Islet of Langerhans.- K-Channels.- A Potassium Channel Modulated by Glucose Metabolism in Rat Pancreatic ?-cells.- Glucose Suppresses ATP-Inhibited K-channels in Pancreatic ?-cells.- K-Channels in an Insulin-secreting Cell Line: Effects of ATP and Sulphonylureas.- Inhibition of K-channels in Insulin Secreting Cells.- Pharmacological Control of 86Rb Efflux from Mouse Pancreatic Islets.- Electrophysiological Measurements Show Marked Differences in the Properties of the Pancreatic ?-cell K-channels from Albino Mice and a Strain of ob/ob (Obese) Mice.- Single K-channel Activity in Fish Islet Cells.- Potassium Channels in Adrenocortical and Parathyroid Cells.- Ca-Channels.- Pharmacological Properties of the Chromaffin Cell Calcium Channel.- Insulin Release and K+-induced Depolarization in Mouse Pancreatic ?-cells.- Calcium and Potassium Currents Recorded from Pancreatic ?-cells Under Voltage Clamp Control.- Voltage-activated Ca2+ and K+ Currents in an Insulin-secreting Cell Line (RINm 5F).- Voltage-gated Ca Current in Pancreatic Islet ?-cells.- Effects of Varapamil and Nifedipine on Glucose-Induced Electrical Activity in Pancreatic ?-cells.- Stimulation of Insulin Release by Organic Calcium-agonists.- Role of Ionic Channels.- Contribution of Isotope Flux Studies to Understanding the Mechanism of the ?-cell Membrane.- 22Na+ Efflux from Normal and ob/ob Mouse Islets of Langerhans.- The Role of Anions in the Regulation of Insulin Secretion.- Graded Spike Electrogenesis in Mouse Pancreatic ?-cell.- Prediction of the Glucose-induced Changes in Membrane Ionic Permeability and Cytosolic Ca2+ by Mathematical Modeling.- Modelling the ?-cell Electrical Activity.- Intracellular Ionized Calcium.- Insulin Secretion Studied in Islets Permeabilised by High Voltage Discharge.- Regulation of Insulin Release Independent of Changes of Cytosolic Ca2+ Concentration.- The Role of Cytosolic Calcium in Insulin Secretion from a Hamster Beta Cell Line.- Differential Effect of Nutrient and Non-nutrient Secretagogues on Cytosolic Free Ca2+ in Pancreatic Islet Cells.- Relationship Between Extracellular Na+ and the Total Ionized Ca2+ Content of Rat Pancreatic Islets.- Neural Regulation.- Mobilization of Different Pools of Glucose-incorporated Calcium in Pancreatic ?-cells After Muscarinic Receptor Activation.- Effect of the Order of Application of Neural Inputs on Insulin Secretion.- Muscarinic Receptors and the Control of Glucose Induced Electrical Activity in the Pancreatic ?-cell.- Electrophysiological Evidence for Histaminergic Modulation of Pancreatic ?-cell Function.- Effect of Melatonin on Insulin Secretion from Isolated Rat Islets of Langerhans.- Mechanisms of Insulin Release.- Calcium Regulation of Membrane Fusion During Hormone Secretion.- The Insulin Secretory Granule: Featurees and Functions in Common with Other Endocrine Granules.- Effects of Monensin on Glucose-induced Insulin Release and 45Ca2+ Outflow.- Interdependency of Ca2+ Availability and Cyclic AMP Generation in the Pancreatic ?-cell.- Cyclic AMP Antagonist, A Second Messenger for Insulin Action, Inhibits Glucose-stimulated Insulin Secretion in Isolated Islets of Chinese Hamsters.- Pulsatile Insulin Release and Electrical Activity from Single ob/ob Mouse Islets of Langerhans.- Comparison of Stimulus-secretion Coupling in Normal and ob/ob (Norwich Colony) Mouse Islets of Langerhans.- Insulin Release, Ca2+ Fluxes and Calmodulin Content of Pancreatic Islet in Aging Rats.- Protein Carboxyl Methylation in Rat Pancreatic Islets. Possible Role in ?-cell Function.- Role of Transglutaminase in Proinsulin Conversion and Insulin Release.- Induction of the Glucokinase-glucose Sensor in Pancreatic Islets of Insulinoma-bearing Rats Following Tumor Removal.- Biochemical Design Features of the Pancreatic Islet Cell Glucose-sensory System.- Contributors.
Pancreatic a-cell biophysics has undergone a veritable informa tion explosion in the past two years. Single channel and macroscopic currents have become easily accessible following the introduction of the patch clamp technique. In addition to this new approach, further development of techniques for optical measurements, ion-sensitive microelectrodes, permeabilized cells and mathematical modelling have recently added to the now classical techniques of membrane potential recording and tracer flux measurement. The International Workshop on Biophysics of the Pancreatic a-Cell held in Alicante (Spain) on Sep tember 30 - October 1, 1985, has now given us the opportunity to share experiences with these new techniques applied to the a-cell. Further more this was the first occasion for most of the groups doing patch clamp studies of the a-cell to decide on appropriate nomenclature and to debate the different characteristics of the a-cell ionic channels. To make this information available to the larger scientific community a record of the meeting has been assembled in this book. It is a collection of research papers by leading scientists at the meet ing working on biophysical, biochemical and physiological aspects. of secretion. We grouped their contributions in seven sections, includ ing new experimental approaches, K-channels, Ca-channels, role of ionic channels, intracellular ionized calcium, neural regulation and mechanisms of insulin release. Each section gives an account of the state of the problem at the time of the meeting, and the subjects are analyzed from the different perspectives of the various contributors.
Springer Book Archives