Describes the mechanisms that support early developmental decisions in the mouse pre-implantationembryoAddresses the structure and function of the three-dimensional space of the nucleus in ES cellsIllustrates the dynamics and regulation of DNA replication in ES cellsAddresses novel genome-wide approaches to analyze splicing and alternative splicing patterns at a globalscale
Describes the mechanisms that support early developmental decisions in the mouse pre-implantation
Addresses the structure and function of the three-dimensional space of the nucleus in ES cells
Illustrates the dynamics and regulation of DNA replication in ES cells
Addresses novel genome-wide approaches to analyze splicing and alternative splicing patterns at a global
Eran Mes horer, PhD, is studying chromatin plasticity in embryonic and neuronal stem cells at the Department of Genetics at the Hebrew University of Jerusalem. He received his PhD in Molecular Neuroscience from the Hebrew University and conducted his post-doctoral studies at the National Cancer Institute, NIH. His lab focuses on understanding pluripotency, differentiation and reprogramming from a chromatin perspective, taking both genome-wide and single cell approaches. He is a member of the International Society for Stem Cell Research and holds the Joseph H. and Belle R. Braun Senior Lectureship in Life Sciences.Kathrin Plath, PhD, is an Assistant Professor in the Department of Biological Chemistry at the University of California Los Angeles since 2004. After she received her PhD from the Humboldt University at Berlin in Germany, she was at the University of California San Francisco and the Whitehead Institute in Cambridge, MA for her postdoctoral studies. Dr. Plath´s main research interest is to understand how developmental cues induce changes in chromatin structure at the molecular level, and how these changes regulate cell fate decisions and gene expression in mammalian development. She is a member of the International Society for Stem Cell Research and of the editorial board of several stem cell journals.
Stem cells have been gaining a lot of attention in recent years. Their unique potential to self-renew and differentiate has turned them into an attractive model for the study of basic biological questions such as cell division, replication, tranillegalscription, cell fate decisions, and more. With embryonic stem (ES) cells that can generate each cell type in the mammalian body and adult stem cells that are able to give rise to the cells within a given lineage, basic questions at different developmental stages can be addressed. Importantly, both adult and embryonic stem cells provide an excellent tool for cell therapy, making stem cell research ever more pertinent to regenerative medicine. As the title The Cell Biology of Stem Cells suggests, our book deals with multiple aspects of stem cell biology, ranging from their basic molecular characteristics to the in vivo stem cell trafficking of adult stem cells and the adult stem-cell niche, and ends with a visit to regeneration and cell fate reprogramming. In the first chapter, "Early embryonic cell fate decisions in the mouse”, Amy Ralson and Yojiro Yamanaka describe the mechanisms that support early developmental decisions in the mouse pre-implantation embryo and the current understanding of the source of the most immature stem cell types, which includes ES cells, trophoblast stem (TS) cells and extraembryonic endoderm stem (XEN) cells.
1. Early Embryonic Cell Fate Decisions in the MouseYojiro Yamanaka and Amy RalstonAbstractIntroductionLineage Establishment and the Pre?stem Cell Program: Formation of the BlastocystLineage Maintenance and the Stem Cell Program: Beyond the BlastocystThe Second Lineage Decision: Subdividing the ICMCell Signaling Regulates PE/EPI SpecificationEstablishment and Modulation of Pluripotency in the EPI LineageConclusion2. Nuclear Architecture in Stem CellsKelly J. Morris, Mita Chotalia and Ana PomboAbstractIntroductionFunctional Compartmentalization of the ES Cell NucleusStem Cell Features of Other Nucleoplasmic SubcompartmentsChromatin Features Characteristic of ES Cell NucleiConclusion3. Epigenetic Regulation of PluripotencyEleni M. Tomazou and Alexander MeissnerAbstractIntroductionEpigenetic ModificationsThe Epigenome of ES CellsConclusion4. Autosomal Lyonization of Replication Domains During Early Mammalian DevelopmentIchiro Hiratani and David M. GilbertAbstractIntroductionReplication Timing Program: An Elusive Measure of Genome OrganizationAn Evolutionarily Conserved Epigenetic FingerprintReplication Timing as a Quantitative Index of 3?Dimensional Genome OrganizationReplication Timing Reveals An Epigenetic Transition: Autosomal Lyonization at the Epiblast StageReplication Timing and Cellular Reprogramming: Further Support for Autosomal LyonizationMaintenance and Alteration of Replication Timing Program and Its Potential RolesConclusion5. PRESERVATION OF GENOMIC INTEGRITY IN MOUSE EMBRYONIC STEM CELLSPeter J. Stambrook and Elisia D. TichyAbstractIntroduction and Historical PerspectiveMutation Frequencies in Somatic CellsProtection of the Mouse ES Cell GenomeConclusion6. Tranillegalscriptional Regulation in Embryonic Stem CellsJian?Chien Dominic Heng and Huck?Hui NgAbstractIntroductionEmbryonic Stem Cells as a Model to Study Tranillegalscriptional RegulationTranillegalscription Factors Governing ESC PluripotencyTranillegalscriptionalRegulatory NetworkTechnologies for Dissecting the Tranillegalscriptional Regulatory NetworkThe Core Tranillegalscriptional Regulatory Network: Oct4, Sox2 and NanogExpanded Tranillegalscriptional Regulatory NetworkEnhanceosomes: Tranillegalscription Factor ComplexIntegration of Signaling Pathways to Tranillegalscriptional NetworkInterface Between Tranillegalscriptional and Epigenetic RegulationConclusion7. ALTERNATIVE SPLICING IN STEM CELL SELF?RENEWAL AND DIFERENTIATIONDavid A. Nelles and Gene W. YeoAbstractIntroductionIntroduction to Alternative SplicingAlternative Splicing of Genes Implicated in Stemness and DifferentiationGenome?Wide Methods to Identify and Detect Alternative Splicing EventsRegulation of Alternative Splicing by RNA Binding ProteinsConclusion and Perspectives8. MicroRNA Regulation of Embryonic Stem Cell Self?Renewal and DifferentiationCollin Melton and Robert BlellochAbstractIntroduction: The Self?Renewal ProgramEmbryonic Stem CellsmiRNA Biogenesis and FunctionESCC miRNAs Promote Self?RenewalmiRNAs Induced during ESC Differentiation Suppress the Self?Renewal ProgramRegulatory Networks Controlling miRNA ExpressionmiRNAs Can Promote or Inhibit Dedifferentiation to iPS CellsmiRNAs in Somatic Stem CellsmiRNAs in Cancer CellsConclusion9. TELOMERES AND TELOMERASE IN ADULT STEM CELLS and PLURIPOTENT EMBRYONIC STEM CELLS Rosa M. Marión and Maria A. BlascoAbstractIntroductionRegulation of Telomeres and TelomeraseRole of Telomeres and Telomerase in Adult SC CompartmentsTelomeres and Telomerase Regulation During Reprogramming by SCNTTelomeres and Telomerase Regulation During iPS Cell GenerationTelomerase Activation is Essential for the "Good” Quality of the Resulting iPS CellsRegulation of Telomere ReprogrammingConclusion10. X Chromosome Inactivation and Embryonic Stem Cells Tahsin Stefan Barakat and Joost GribnauAbstractIntroductionCis Acting Factors in XCITrans Acting Factors in XCICounting and ChoiceSilencing and Maintenance of
The Cell Biology of Stem Cells discusses multiple aspects of stem cell biology, ranging from the basic molecular characteristics to the in vivo stem cell trafficking of adult stem cells. The cell biology of stem cells is discussed, in addition to the questions that still remain.
Stem cells have been gaining a lot of attention in recent years. Their unique potential to self-renew and differentiate has turned them into an attractive model for the study of basic biological questions such as cell division, replication, tranillegalscription, cell fate decisions, and more. With embryonic stem (ES) cells that can generate each cell type in the mammalian body and adult stem cells that are able to give rise to the cells within a given lineage, basic questions at different developmental stages can be addressed. Importantly, both adult and embryonic stem cells provide an excellent tool for cell therapy, making stem cell research ever more pertinent to regenerative medicine. As the title The Cell Biology of Stem Cells suggests, our book deals with multiple aspects of stem cell biology, ranging from their basic molecular characteristics to the in vivo stem cell trafficking of adult stem cells and the adult stem-cell niche, and ends with a visit to regeneration and cell fate reprogramming. In the first chapter, "Early embryonic cell fate decisions in the mouse", Amy Ralson and Yojiro Yamanaka describe the mechanisms that support early developmental decisions in the mouse pre-implantation embryo and the current understanding of the source of the most immature stem cell types, which includes ES cells, trophoblast stem (TS) cells and extraembryonic endoderm stem (XEN) cells.
Early Embryonic Cell Fate Decisions in the Mouse.- Nuclear Architecture in Stem Cells.- Epigenetic Regulation of Pluripotency.- Autosomal Lyonization of Replication Domains During Early Mammalian Development.- Preservation of Genomic Integrity in Mouse Embryonic Stem Cells.- Tranillegalscriptional Regulation in Embryonic Stem Cells.- Alternative Splicing in Stem Cell Self-Renewal and Diferentiation.- MicroRNA Regulation of Embryonic Stem Cell Self-Renewal and Differentiation.- Telomeres And Telomerase in Adult Stem Cells and Pluripotent Embryonic Stem Cells.- X Chromosome Inactivation and Embryonic Stem Cells.- Adult Stem Cels and Their Niches.- Adult Stem Cel Diferentiation and Trafficking And Their Implications in Disease.- Vertebrates That Regenerate As Models For Guiding Stem Cels.- Reprogramming of Somatic Cells to Pluripotency.
Eran Mes horer, PhD, is studying chromatin plasticity in embryonic and neuronal stem cells at the Department of Genetics at the Hebrew University of Jerusalem. He received his PhD in Molecular Neuroscience from the Hebrew University and conducted his post doctoral studies at the National Cancer Institute, NIH. His lab focuses on understanding pluripotency, differentiation and reprogramming from a chromatin perspective, taking both genome wide and single cell approaches. He is a member of the International Society for Stem Cell Research and holds the Joseph H. and Belle R. Braun Senior Lectureship in Life Sciences.Kathrin Plath, PhD, is an Assistant Professor in the Department of Biological Chemistry at the University of California Los Angeles since 2004. After she received her PhD from the Humboldt University at Berlin in Germany, she was at the University of California San Francisco and the Whitehead Institute in Cambridge, MA for her postdoctoral studies. Dr. Plath's main research interest is to understand how developmental cues induce changes in chromatin structure at the molecular level, and how these changes regulate cell fate decisions and gene expression in mammalian development. She is a member of the International Society for Stem Cell Research and of the editorial board of several stem cell journals.
Über den Autor
Eran Mes horer, PhD, is studying chromatin plasticity in embryonic and neuronal stem cells at the Department of Genetics at the Hebrew University of Jerusalem. He received his PhD in Molecular Neuroscience from the Hebrew University and conducted his post¿doctoral studies at the National Cancer Institute, NIH. His lab focuses on understanding pluripotency, differentiation and reprogramming from a chromatin perspective, taking both genome¿wide and single cell approaches. He is a member of the International Society for Stem Cell Research and holds the Joseph H. and Belle R. Braun Senior Lectureship in Life Sciences.
Kathrin Plath, PhD, is an Assistant Professor in the Department of Biological Chemistry at the University of California Los Angeles since 2004. After she received her PhD from the Humboldt University at Berlin in Germany, she was at the University of California San Francisco and the Whitehead Institute in Cambridge, MA for her postdoctoral studies. Dr. Plath's main research interest is to understand how developmental cues induce changes in chromatin structure at the molecular level, and how these changes regulate cell fate decisions and gene expression in mammalian development. She is a member of the International Society for Stem Cell Research and of the editorial board of several stem cell journals.
Inhaltsverzeichnis
Early Embryonic Cell Fate Decisions in the Mouse.- Nuclear Architecture in Stem Cells.- Epigenetic Regulation of Pluripotency.- Autosomal Lyonization of Replication Domains During Early Mammalian Development.- Preservation of Genomic Integrity in Mouse Embryonic Stem Cells.- Tranillegalscriptional Regulation in Embryonic Stem Cells.- Alternative Splicing in Stem Cell Self-Renewal and Diferentiation.- MicroRNA Regulation of Embryonic Stem Cell Self-Renewal and Differentiation.- Telomeres And Telomerase in Adult Stem Cells and Pluripotent Embryonic Stem Cells.- X Chromosome Inactivation and Embryonic Stem Cells.- Adult Stem Cels and Their Niches.- Adult Stem Cel Diferentiation and Trafficking And Their Implications in Disease.- Vertebrates That Regenerate As Models For Guiding Stem Cels.- Reprogramming of Somatic Cells to Pluripotency.
Klappentext
Stem cells have been gaining a lot of attention in recent years. Their unique potential to self-renew and differentiate has turned them into an attractive model for the study of basic biological questions such as cell division, replication, tranillegalscription, cell fate decisions, and more. With embryonic stem (ES) cells that can generate each cell type in the mammalian body and adult stem cells that are able to give rise to the cells within a given lineage, basic questions at different developmental stages can be addressed. Importantly, both adult and embryonic stem cells provide an excellent tool for cell therapy, making stem cell research ever more pertinent to regenerative medicine. As the title The Cell Biology of Stem Cells suggests, our book deals with multiple aspects of stem cell biology, ranging from their basic molecular characteristics to the in vivo stem cell trafficking of adult stem cells and the adult stem-cell niche, and ends with a visit to regeneration and cell fate reprogramming. In the first chapter, ¿Early embryonic cell fate decisions in the mouse¿, Amy Ralson and Yojiro Yamanaka describe the mechanisms that support early developmental decisions in the mouse pre-implantation embryo and the current understanding of the source of the most immature stem cell types, which includes ES cells, trophoblast stem (TS) cells and extraembryonic endoderm stem (XEN) cells.
Describes the mechanisms that support early developmental decisions in the mouse pre-implantation
Addresses the structure and function of the three-dimensional space of the nucleus in ES cells
Illustrates the dynamics and regulation of DNA replication in ES cells
Addresses novel genome-wide approaches to analyze splicing and alternative splicing patterns at a global
