1: Detection And Analysis Of Proteins A. Introduction B. Basic methods for protein analysisC. Characterization of primary, secondary, tertiary and quaternary structures of proteins2: Detection And Analysis Of Nucleic Acids A. Introduction B. Basic methods for nucleic acid analysis C. DNA sequencing D. Detection and quantitation of RNA3: Recombinant DNA Techniques: Cloning And Manipulation Of DNA A. Introduction B. Plasmid and viral vectors C. Libraries D. Site Directed Mutagenesis E. Polymerase chain reaction (PCR) F. Screening for differentially expressed genes (DEGs) G. Promoter-protein interactionsH. Silencing gene expression I. Forensics and DNA technology 4: Antibody-Based Techniques A. Introduction B. Monoclonal antibodies C. Purification and use of antibodies D. Flow cytometry and fluorescence activated cell sorting (FACS) E. Other assays using antibodies5: Microscopy: Imaging Of Biological Specimens A. IntroductionB. Light microscopyC. Fluorescence and laser confocal microarray microscopy D. Electron Microscopy (EM)E. Magnetic resonance imaging (MRI)6: The Derivation And Manipulation Of Experimental Animals In Biomedical SciencesA. Introduction B. Inbred strains of miceC. Congenic strains of mice D. Transgenic mice E. Targeted transgenes: "knockout" and "knockin" mice F. Other uses of mice in biomedical research References Some excellent methodology manualsIndex
Thousands of methods have been developed in the various biomedical disciplines, and those covered in this book represent the basic, essential and most widely used methods in several different disciplines.
Thousands of methods have been developed in the various biomedical disciplines and those covered in this book represent the basic, essential and most widely used methods in several different disciplines.