Chapter 1- Introduction
Chapter 2- Antibodies
Chapter 3- Sample Preparation/Fixation
Chapter 4- Tissue Embedding
Chapter 5- Blocking and Permeability
Chapter 6- Labels for Antibodies
Chapter 7- Application Methods
Chapter 8- Controls
Chapter 9- Method and Label Decision
Chapter 10- Single Antibody Procedure
Chapter 11- Multiple Antibodies Different Species
Chapter 12- Multiple Antibodies Same Species
Chapter 13- Microscopy and Images
Chapter 14- Trouble-Shooting
Chapter 15- Electron Microscopic Immunocytochemistry
In biomedical research, because of a dramatic increase in productivity, immunocytochemistry has emerged as a major technique. The proposed book will provide the first practical guide to planning, performing, and evaluating immunocytochemical experiments.
In today's graduate education the emphasis is on doing research and not on formal class work. Graduate students therefore lack the background in many essential techniques necessary to perform research in fields in which they were not trained. As director of a university core microscopy facility which sees students and faculty from dozens of laboratories each year, Dr. Burry has surmised the vast majority of these novice microscope users need considerable help. In an attempt to educate users, Dr. Burry has initiated immunocytochemistry seminars and workshops which serve to train people in this powerful research tool. The proposed book is an outgrowth of these presentations and conversations with, by now, hundreds of people who have asked for help.
The philosophy which separates this book from other books in this field is that it is practical, rather than academic. In looking at other important immunocytochemistry titles, the predominant orientation is academic, with the author attempting to comprehensively discuss the topic. For example, one book with sample preparation lists ten fixatives which can be used; however, only two such fixatives are commonly used today. In this particular title, the detailed discussion of old methods might be seen as important in establishing the author as an expert. By contrast, the approach for Burry's book would be to discuss methods based on what works in animal research laboratories today, and focus only on the most productive methods.
An additional distinction with this proposed book is the focus on animal research and
The first practical guide to planning, performing, and evaluating immunocytochemical experiments
Emphasis on practical, rather than academic, focusing only on the most productive methods
Strong focus on animal research over human pathology, as processing for animal-based tissues takes about a third of the time and results in higher quality images
Because this field is new and is emerging, there are numerous advantages of specific methods over other, more generalized methods
The purpose of this book is to show a novice how to do immunocytochemistry without engaging in a discussion of possible advanced methods