Chapter 1. The Polymerase Chain Reaction Chapter 2. A Brief Comparison Between In Vivo DNA Replication And In Vitro PCR Amplification Chapter 3. The PCR In Practice Chapter 4. The Different Types And Varieties Of Nucleic Acid Target Molecules Chapter 5: PCR Primers Chapter 6. Deoxynucleotide Triphosphates And Buffer Components Chapter 7. Taq And Other Thermostable DNA Polymerases Chapter 8. Important Considerations For Typical, Quantitative And Real-Time PCR Protocols Chapter 9. Analysis Of PCR Amplification Products. Chapter 10. Ensuring PCR Quality - Laboratory Organisation, Pcr Optimisation And Controls. Chapter 11. Ensuring PCR Quality-Quality Criteria And Quality Assurance. Chapter 12. Variants And Adaptations Of The Standard Pcr Protocol Chapter 13. In Situ PCR Amplification (Isa) - Major Considerations, Sample Processing And Applications
Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).
Gives a broad overview of available PCR techniques and variants
Focussing on the PCR technique, not restricted to a few limited applications associated with a minor discipline
Chapters outlining appropriate quality control procedures are included
Written by scientists with a broad knowledge of the field of PCR techniques and PCR-related clinical research