Contributing AuthorsnPrefacenAcknowledgements Nucleic acids hybridization: potentials and limitations nAnton Buzdin Selective suppression of polymerase chain reaction and its most popular applications nSergey Lukyanov, Konstantin Lukyanov, Nadezhda Gurskaya, Ekaterina Bogdanova, Anton Buzdin Suppression subtractive hybridization nSergey Lukyanov, Denis Rebrikov, Anton Buzdin Stem-loop oligonucleotides as hybridization probes and their practical use in molecular biology and biomedicine nAnton Buzdin, Sergey Lukyanov Generation of normalized cDNA librariesnAlex Shcheglov, Pavel Zhulidov, Ekaterina Bogdanova, Dmitry Shagin Primer extension enrichment reaction (PEER) and other methods for difference screening nLilia Ganova-Raeva Subtractive hybridization with covalently modified oligonucleotides nShi-Lung Lin, Donald Chang, Joseph Miller and Shao-Yao Ying Coincidence cloning: robust technique for isolation of common sequencesnAnton Buzdin DNA hybridization in solution for mutation detection nAnton Buzdin Current attempts to improve the specificity of nucleic acids hybridization nAnton Buzdin Concepts on microarray design for genome and transcriptome analyses nHelder I. Nakaya, Eduardo M. Reis and Sergio Verjovski-Almeida Index
Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and biomedicine, while the potential of many other advantageous techniques seems to be underestimated. Written by an international team of authors, this book details the current state-of-art in hybridization techniques. Coverage includes experimental protocols along with comprehensive and detailed method descriptions.
In-depth review of nucleic acid hybridization in vitro techniques
All major applications covered
Includes novel methods with the potential of being widely used
Detailed experimental protocols provided