Very first book dedicated to the proteomic analysis of subcellular structures
Written by a panel of acknowledged international experts in the field
Contains comprehensive reviews on membrane proteomics, organelle subproteomes and supramolecular complexes
Surveys the latest breakthroughs in subcellular fractionation and proteomics
Covers a wide range of topics including: signalling mechanisms, systems biology and emerging technologies in proteomics
This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organization and their specific methodologies. In addition, the book includes coverage of systems biology that deals with the integration of the data derived from these different levels to produce a synthetic deillegalscription of the cell as a system.
Introduction Section 1: Membrane proteomicsKeynotes on membrane proteomicsThierry Rabilloud Two-dimensional BAC/SDS-PAGE for membrane proteomicsRené P. Zahedi, Jan Moebius and Albert Sickmann Microparticles: a new tool for plasma membrane sub-cellular proteomicLaurent Miguet, Sarah Sanglier, Christine Schaeffer, Noelle Potier, Laurent Mauvieux and Alain Van Dorsselaer. Lipid raft proteomics: more than just detergent-resistant membranesLeonard J. Foster and Queenie W. T. Chan Section 2: Organelle subproteomesOrganelle Proteome Variation Among Different Cell Types: Lessons from Nuclear Membrane ProteinsDeirdre M. Kavanagh, William E. Powell, Poonam Malik, Vassiliki Lazou and Eric C. Schirmer Synaptosome ProteomicsFengju Bai and Frank A. Witzmann Proteomic analysis of secreted exosomesChristine Olver and Michel Vidal Section 3: Characterization of supramolecular protein complexesFrom Protein-Protein Complexes to InteractomicsVincent Collura and Guillaume Boissy Supramolecular signalling complexes in the nervous systemMark O. Collins and Seth G.N. Grant Protein networks and complexes in photoreceptor ciliaRonald Roepman and Uwe Wolfrum Section 4: Subcellular systems biologySystems Biology and the Reconstruction of the Cell: from molecular components to integral functionFrank J. Bruggeman, Sergio Rossell, Karen van Eunen, Jildau Bouwman, Hans V. Westerhoff and Barbara Bakker Automated, Systematic Determination of Protein Subcellular Location using Fluorescence MicroscopyElvira García Osuna and Robert F. Murphy Systems biology of the Endoplasmic Reticulum stressresponseMarie-Elaine Caruso and Eric Chevet Section 5: Emerging technologies in proteomicsSystems Nanobiology: From Quantitative Single Molecule Biophysics to Microfluidic-Based Single Cell AnalysisJoerg Martini, Wibke Hellmich, Dominik Greif, Anke Becker, Thomas Merkle, Robert Ros, Alexandra Ros, Katja Toensing and Dario Anselmetti Biophotonics applied to proteomicsMichel Faupel, Débora Bonenfant, Patrick Schindler, Eric Bertrand Dieter Mueller, Markus Stoeckli, Francis Bitsch, Tatiana Rohner, Dieter Staab and Jan Van Oostrum Differential epitope identification of antibodies against intracellular domains of Alzheimer`s amyloid precursor protein using high resolution affinity- mass spectrometryXiaodan Tian, Madalina Maftei, Markus Kohlmann, Bernadette Allinquant and Michael Przybylski LC-MALDI MS and MS/MS – An Efficient Tool in Proteome Analysis Dieter R. Mueller, Hans Voshol, Annick Waldt, Brigitte Wiedmann and Jan van Oostrum
This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organization and their specific methodologies. In addition, the book includes coverage of systems biology that deals with the integration of the data derived from these different levels to produce a synthetic deillegalscription of the cell as a system.
Membrane Proteomics.- Keynotes on Membrane Proteomics.- Two-Dimensional BAC/SDS-PAGE for Membrane Proteomics.- Microparticles: A New Tool for Plasma Membrane Sub-cellular Proteomic.- Lipid Raft Proteomics: More than Just Detergent-Resistant Membranes.- Organelle Subproteomes.- Organelle Proteome Variation Among Different Cell Types: Lessons from Nuclear Membrane Proteins.- Synaptosome Proteomics.- Proteomic Analysis of Secreted Exosomes.- Characterization of Supramolecular Protein Complexes.- From Protein-Protein Complexes to Interactomics.- Supramolecular Signalling Complexes in the Nervous System.- Protein Networks and Complexes in Photoreceptor Cilia.- Subcellular Systems Biology.- Systems Biology and the Reconstruction of the Cell: From Molecular Components to Integral Function.- Automated, Systematic Determination of Protein Subcellular Location using Fluorescence Microscopy.- Systems Biology of the Endoplasmic Reticulum Stress Response.- Emerging Technologies in Proteomics.- Systems Nanobiology: From Quantitative Single Molecule Biophysics to Microfluidic-Based Single Cell Analysis.- Biophotonics Applied to Proteomics.- Differential Epitope Identification of Antibodies Against Intracellular Domains of Alzheimer's Amyloid Precursor Protein Using High Resolution Affinity-mass Spectrometry.- LC-MALDI MS and MS/MS - An Efficient Tool in Proteome Analysis.
Inhaltsverzeichnis
Introduction Section 1: Membrane proteomics
Keynotes on membrane proteomics
Thierry Rabilloud Two-dimensional BAC/SDS-PAGE for membrane proteomics
René P. Zahedi, Jan Moebius and Albert Sickmann Microparticles: a new tool for plasma membrane sub-cellular proteomic
Laurent Miguet, Sarah Sanglier, Christine Schaeffer, Noelle Potier, Laurent Mauvieux and Alain Van Dorsselaer. Lipid raft proteomics: more than just detergent-resistant membranes
Leonard J. Foster and Queenie W. T. Chan Section 2: Organelle subproteomes
Organelle Proteome Variation Among Different Cell Types: Lessons from Nuclear Membrane Proteins
Deirdre M. Kavanagh, William E. Powell, Poonam Malik, Vassiliki Lazou and Eric C. Schirmer Synaptosome Proteomics
Fengju Bai and Frank A. Witzmann Proteomic analysis of secreted exosomes
Christine Olver and Michel Vidal Section 3: Characterization of supramolecular protein complexes
From Protein-Protein Complexes to Interactomics
Vincent Collura and Guillaume Boissy Supramolecular signalling complexes in the nervous system
Mark O. Collins and Seth G.N. Grant Protein networks and complexes in photoreceptor cilia
Ronald Roepman and Uwe Wolfrum Section 4: Subcellular systems biology
Systems Biology and the Reconstruction of the Cell: from molecular components to integral function
Frank J. Bruggeman, Sergio Rossell, Karen van Eunen, Jildau Bouwman, Hans V. Westerhoff and Barbara Bakker Automated, Systematic Determination of Protein Subcellular Location using Fluorescence Microscopy
Elvira García Osuna and Robert F. Murphy Systems biology of the Endoplasmic Reticulum stressresponse
Marie-Elaine Caruso and Eric Chevet Section 5: Emerging technologies in proteomics
Systems Nanobiology: From Quantitative Single Molecule Biophysics to Microfluidic-Based Single Cell Analysis
Joerg Martini, Wibke Hellmich, Dominik Greif, Anke Becker, Thomas Merkle, Robert Ros, Alexandra Ros, Katja Toensing and Dario Anselmetti Biophotonics applied to proteomics
Michel Faupel, Débora Bonenfant, Patrick Schindler, Eric Bertrand Dieter Mueller, Markus Stoeckli, Francis Bitsch, Tatiana Rohner, Dieter Staab and Jan Van Oostrum Differential epitope identification of antibodies against intracellular domains of Alzheimer`s amyloid precursor protein using high resolution affinity- mass spectrometry
Xiaodan Tian, Madalina Maftei, Markus Kohlmann, Bernadette Allinquant and Michael Przybylski LC-MALDI MS and MS/MS - An Efficient Tool in Proteome Analysis
Dieter R. Mueller, Hans Voshol, Annick Waldt, Brigitte Wiedmann and Jan van Oostrum
Klappentext
This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organization and their specific methodologies. In addition, the book includes coverage of systems biology that deals with the integration of the data derived from these different levels to produce a synthetic deillegalscription of the cell as a system.
Very first book dedicated to the proteomic analysis of subcellular structures
Written by a panel of acknowledged international experts in the field
Contains comprehensive reviews on membrane proteomics, organelle subproteomes and supramolecular complexes
Surveys the latest breakthroughs in subcellular fractionation and proteomics
Covers a wide range of topics including: signalling mechanisms, systems biology and emerging technologies in proteomics
