Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field.
1. Methods of Development of Biparental Mapping Populations in Horticultural Crops
Pasquale Tripodi
2. Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits
Isidore Diouf and Laura Pascual
3. Using Metabolomics to Assist Plant Breeding
Saleh Alseekh and Alisdair R. Fernie
4. High Throughput DNA Isolation in Vegetable Crops for Genomics Applications
Pasquale Tripodi and Giovanna Festa
5. High Resolution Melting Analysis as a Tool for Plant Species Authentication
Liliana Grazina, Joana Costa, Joana S. Amaral, and Isabel Mafra
6. Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping Methods
Zhangsheng Zhu, Binmei Sun, and Jianjun Lei
7. Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome
Fernando J. Yuste-Lisbona, Jose M. Jimenez-Gomez, Carmen Capel, and Rafael Lozano
8. Association Mapping in Plants
Pawan L. Kulwal and Ravinder Singh
9. Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs)
Contaldi Felice, Cappetta Elisa, and Esposito Salvatore
10. Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis Teresa Rosa Galise, Salvatore Esposito, and Nunzio D´Agostino
11. RNA Interference (RNAi) in Tomato Crop Reseserch
Pasquale Termolino
12. Protoplast Based Method for Genome Editing in Tetraploid Potato
Alessandro Nicolia, Ann-Sofie Fält, Per Hofvander, and Mariette Andersson
13. The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronaria
Andrea Copetta and Marina Laura
14. Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant
Edgar García-Fortea, Ana García-Pérez, Esther Gimeno-Páez, Marina Martínez-López, Santiago Vilanova, Pietro Gramazio, Jaime Prohens and Mariola Plazas
15. Assembly of TALEN and mTALE-Act for Plant Genome Engineering
Aimee A. Malzahn
and Yiping Qi
16. Genome Editing to Achieve the Crop Ideotype in Tomato
Tomaš Cermák, Karla Gasparini, Zoltán Kevei,
and Agustin Zsögön
17. Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging
Gernot Bodner, Mouhannad Alsalem, and Alireza Nakhforoosh
18. Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis
Federico Pallottino, Simone Figorilli, Cristina Cecchini, and Corrado Costa
This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field.
Methods of Development of Biparental Mapping Populations in Horticultural Crops.- Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits.- Using Metabolomics to Assist Plant Breeding.- High Throughput DNA Isolation in Vegetable Crops for Genomics Applications.- High Resolution Melting Analysis as a Tool for Plant Species Authentication.- Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping Methods.- Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome.- Association Mapping in Plants.- Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs).- Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis.- RNA Interference (RNAi) in Tomato Crop Reseserch.- Protoplast Based Method for Genome Editing in Tetraploid Potato.- The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronaria.- Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant.- Assembly of TALEN and mTALE-Act for Plant Genome Engineering.- Genome Editing to Achieve the Crop Ideotype in Tomato.- Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging.- Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis.
Inhaltsverzeichnis
1. Methods of Development of Biparental Mapping Populations in Horticultural Crops
Pasquale Tripodi
2. Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits
Isidore Diouf and Laura Pascual
3. Using Metabolomics to Assist Plant Breeding
Saleh Alseekh and Alisdair R. Fernie
4. High Throughput DNA Isolation in Vegetable Crops for Genomics Applications
Pasquale Tripodi and Giovanna Festa
5. High Resolution Melting Analysis as a Tool for Plant Species Authentication
Liliana Grazina, Joana Costa, Joana S. Amaral, and Isabel Mafra
6. Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping Methods
Zhangsheng Zhu, Binmei Sun, and Jianjun Lei
7. Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome
Fernando J. Yuste-Lisbona, Jose¿ M. Jime¿nez-Gömez, Carmen Capel, and Rafael Lozano
8. Association Mapping in Plants
Pawan L. Kulwal and Ravinder Singh
9. Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs)
Contaldi Felice, Cappetta Elisa, and Esposito Salvatore
10. Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis Teresa Rosa Galise, Salvatore Esposito, and Nunzio D¿Agostino
11. RNA Interference (RNAi) in Tomato Crop Reseserch
Pasquale Termolino
12. Protoplast Based Method for Genome Editing in Tetraploid Potato
Alessandro Nicolia, Ann-Sofie Fält, Per Hofvander, and Mariette Andersson
13. The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronaria
Andrea Copetta and Marina Laura
14. Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant
Edgar García-Fortea, Ana García-Pérez, Esther Gimeno-Páez, Marina Martínez-López, Santiago Vilanova, Pietro Gramazio, Jaime Prohens and Mariola Plazas
15. Assembly of TALEN and mTALE-Act for Plant Genome Engineering
Aimee A. Malzahn
and Yiping Qi
16. Genome Editing to Achieve the Crop Ideotype in Tomato
Tomaš ¿ermák, Karla Gasparini, Zoltán Kevei,
and Agustin Zsögön
17. Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging
Gernot Bodner, Mouhannad Alsalem, and Alireza Nakhforoosh
18. Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis
Federico Pallottino, Simone Figorilli, Cristina Cecchini, and Corrado Costa
Klappentext
This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.nnn nnnAuthoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
