Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.
The chapter "Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Part I: Fundamental Aspects of Cryopreservation and Freeze-Drying
1. Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues
Willem F. Wolkers and Harriëtte Oldenhof
2. Principles of Ice-Free Cryopreservation by Vitrification
Gregory M. Fahy and Brian Wowk
3. The Principles of Freeze-Drying and Application of Analytical Technologies
Kevin R. Ward and Paul Matejtschuk
4. Mathematical Modeling and Optimization of Cryopreservation in Single Cells
James D. Benson
5. Mathematical Modeling of Protectant Transport in Tissues
Ross M. Warner and Adam Z. Higgins
Part II: Technologies and Methods to Study Freezing and Drying
6. Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation
Peter Kilbride and Julie Meneghel
7. Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of Biologics
Shangping Wang, Susan Trammell, and Gloria D. Elliott
8. High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kinetics
Jens O.M. Karlsson
9. Use of Ice Recrystallization Inhibition Assays to Screen for Compounds that Inhibit Ice Recrystallization
Anna A. Ampaw, August Sibthorpe, and Robert N. Ben
10. DSC Analysis of Thermophysical Properties for Biomaterials and Formulations
Wendell Q. Sun
11. Osmometric Measurements of Cryoprotective Agent Permeation into Tissues
Kezhou Wu, Leila Laouar, Nadia Shardt, Janet A.W. Elliott, and Nadr M. Jomha
12. Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes
Ariadna Corral, Alberto Olmo, and Ramón Risco
13. Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological Research
Willem F. Wolkers and Harriëtte Oldenhof
14. Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements
Guanglin Yu, Rui Li, and Allison Hubel
Part III: Cryopreservation and Freeze-Drying Protocols
15. Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm
Harriëtte Oldenhof, Willem F. Wolkers, and Harald Sieme
16. Cryopreservation of Avian Semen
Henri Woelders
17. Cryopreservation of Mouse Sperm for Genome Banking
Yuksel Agca and Cansu Agca
18. Cryopreservation of Marine Invertebrates: From Sperm to Complex Larval Stages
Estefania Paredes, Pablo Heres, Catarina Anjos, and Elsa Cabrita
19. Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into a Cooling Agent
Mengying Wang, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, and Vladimir Isachenko
20. Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification as Successful Methods for Cryogenic Storage
Victoria Keros and Barry J. Fuller
21. Vitrification of Porcine Oocytes and Zygotes in Microdrops on a Solid Metal Surface or Liquid Nitrogen
Tamas Somfai and Kazuhiro Kikuchi
22. Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research
Yuksel Agca and Cansu Agca
23. Cryopreservation and Thawing of Human Ovarian Cortex Tissue Slices Jana Liebenthron and Markus Montag
24. Vitrification: A Simple and Successful Method for Cryostorage of Human Blastocysts
Juergen Liebermann
25. Vitrification of Equine In Vivo-Derived Embryos, after Blastocoel Aspiration
Carolina Herrera
26. Frozen Blood Reserves
Johan W. Lagerberg
27. Isolation, Cryopreservation, and Characterization of iPSC-Derived Megakaryocytes
Denys Pogozhykh, Rainer Blasczyk, and Constança Figueiredo
28. Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem Cells
Melany López and Ali Eroglu
29. Chemically-Defined and Xeno-Free Cryopreservation of Human Induced Pluripotent Stem Cells
Juliette Seremak and Ali Eroglu
30. Protocol for Cryopreservation of Endothelial Monolayers
Leah A. Marquez-Curtis, Nasim Eskandari, Locksley E. McGann, and Janet A.W. Elliott
31. Vitrification of Heart Valve Tissues
Kelvin G.M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene, and Lia H. Campbell
32. Cryopreservation of Algae
Estefania Paredes, Angela Ward, Ian Probert, Léna Gouhier, and Christine N. Campbell
33. Cryopreservation of Fern Spores and Pollen
Anna Nebot, Victoria J. Philpott, Anna Pajdo, and Daniel Ballesteros
34. Cryopreservation of Plant Cell Lines Using Alginate Encapsulation
Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack
35. Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3
Angelika Senula and Manuela Nagel
36. Cryopreservation of Seeds and Seed Embryos in Orthodox, Intermediate, and Recalcitrant Seeded Species
Daniel Ballesteros, Natalia Fanega-Sleziak, and Rachael Davies
37. Freeze-Drying of Proteins
Baolin Liu and Xinli Zhou
38. Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical Properties
Fernanda Fonseca, Amélie Girardeau, and Stéphanie Passot
39. Preservation of Mammalian Sperm by Freeze-Drying
Levent Keskintepe and Ali Eroglu
40. Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf Availability
Willem F. Wolkers and Andres Hilfiker
This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.
The chapter "Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and authoritative,
Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.
The chapter "Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues.- Principles of Ice-Free Cryopreservation by Vitrification.- The Principles of Freeze-Drying and Application of Analytical Technologies.- Mathematical Modeling and Optimization of Cryopreservation in Single Cells.- Mathematical Modeling of Protectant Transport in Tissues.- Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation.- Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of Biologics.- High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kinetics.- Use of Ice Recrystallization Inhibition Assays to Screen for Compounds that Inhibit Ice Recrystallization.- DSC Analysis of Thermophysical Properties for Biomaterials and Formulations.- Osmometric Measurements of Cryoprotective Agent Permeation into Tissues.- Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes.- Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological Research.- Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements.- Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm.- Cryopreservation of Avian Semen.- Cryopreservation of Mouse Sperm for Genome Banking.- Cryopreservation of Marine Invertebrates: From Sperm to Complex Larval Stages.- Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into a Cooling Agent.- Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification as Successful Methods for Cryogenic Storage.- Vitrification of Porcine Oocytes and Zygotes in Microdrops on a Solid Metal Surface or Liquid Nitrogen.- Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research.- Cryopreservation and Thawing of Human Ovarian Cortex Tissue Slices.- Vitrification: A Simple and Successful Method for Cryostorage of Human Blastocysts.- Vitrificationof Equine In Vivo-Derived Embryos, after Blastocoel Aspiration.- Frozen Blood Reserves.- Isolation, Cryopreservation, and Characterization of iPSC-Derived Megakaryocytes.- Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem Cells.- Chemically-Defined and Xeno-Free Cryopreservation of Human Induced Pluripotent Stem Cells.- Protocol for Cryopreservation of Endothelial Monolayers.- Vitrification of Heart Valve Tissues.- Cryopreservation of Algae.- Cryopreservation of Fern Spores and Pollen.- Cryopreservation of Plant Cell Lines Using Alginate Encapsulation.- Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3.- Cryopreservation of Seeds and Seed Embryos in Orthodox, Intermediate, and Recalcitrant Seeded Species.- Freeze-Drying of Proteins.- Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical Properties.- Preservation of Mammalian Sperm by Freeze-Drying.- Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf Availability.
Inhaltsverzeichnis
Part I: Fundamental Aspects of Cryopreservation and Freeze-Drying
1. Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues
Willem F. Wolkers and Harriëtte Oldenhof
2. Principles of Ice-Free Cryopreservation by Vitrification
Gregory M. Fahy and Brian Wowk
3. The Principles of Freeze-Drying and Application of Analytical Technologies
Kevin R. Ward and Paul Matejtschuk
4. Mathematical Modeling and Optimization of Cryopreservation in Single Cells
James D. Benson
5. Mathematical Modeling of Protectant Transport in Tissues
Ross M. Warner and Adam Z. Higgins
Part II: Technologies and Methods to Study Freezing and Drying
6. Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation
Peter Kilbride and Julie Meneghel
7. Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of Biologics
Shangping Wang, Susan Trammell, and Gloria D. Elliott
8. High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kinetics
Jens O.M. Karlsson
9. Use of Ice Recrystallization Inhibition Assays to Screen for Compounds that Inhibit Ice Recrystallization
Anna A. Ampaw, August Sibthorpe, and Robert N. Ben
10. DSC Analysis of Thermophysical Properties for Biomaterials and Formulations
Wendell Q. Sun
11. Osmometric Measurements of Cryoprotective Agent Permeation into Tissues
Kezhou Wu, Leila Laouar, Nadia Shardt, Janet A.W. Elliott, and Nadr M. Jomha
12. Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes
Ariadna Corral, Alberto Olmo, and Ramón Risco
13. Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological Research
Willem F. Wolkers and Harriëtte Oldenhof
14. Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements
Guanglin Yu, Rui Li, and Allison Hubel
Part III: Cryopreservation and Freeze-Drying Protocols
15. Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm
Harriëtte Oldenhof, Willem F. Wolkers, and Harald Sieme
16. Cryopreservation of Avian Semen
Henri Woelders
17. Cryopreservation of Mouse Sperm for Genome Banking
Yuksel Agca and Cansu Agca
18. Cryopreservation of Marine Invertebrates: From Sperm to Complex Larval Stages
Estefania Paredes, Pablo Heres, Catarina Anjos, and Elsa Cabrita
19. Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into a Cooling Agent
Mengying Wang, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, and Vladimir Isachenko
20. Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification as Successful Methods for Cryogenic Storage
Victoria Keros and Barry J. Fuller
21. Vitrification of Porcine Oocytes and Zygotes in Microdrops on a Solid Metal Surface or Liquid Nitrogen
Tamas Somfai and Kazuhiro Kikuchi
22. Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research
Yuksel Agca and Cansu Agca
23. Cryopreservation and Thawing of Human Ovarian Cortex Tissue Slices
Jana Liebenthron and Markus Montag
24. Vitrification: A Simple and Successful Method for Cryostorage of Human Blastocysts
Juergen Liebermann
25. Vitrification of Equine In Vivo-Derived Embryos, after Blastocoel Aspiration
Carolina Herrera
26. Frozen Blood Reserves
Johan W. Lagerberg
27. Isolation, Cryopreservation, and Characterization of iPSC-Derived Megakaryocytes
Denys Pogozhykh, Rainer Blasczyk, and Constança Figueiredo
28. Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem Cells
Melany López and Ali Eroglu
29. Chemically-Defined and Xeno-Free Cryopreservation of Human Induced Pluripotent Stem Cells
Juliette Seremak and Ali Eroglu
30. Protocol for Cryopreservation of Endothelial Monolayers
Leah A. Marquez-Curtis, Nasim Eskandari, Locksley E. McGann, and Janet A.W. Elliott
31. Vitrification of Heart Valve Tissues
Kelvin G.M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene, and Lia H. Campbell
32. Cryopreservation of Algae
Estefania Paredes, Angela Ward, Ian Probert, Léna Gouhier, and Christine N. Campbell
33. Cryopreservation of Fern Spores and Pollen
Anna Nebot, Victoria J. Philpott, Anna Pajdo, and Daniel Ballesteros
34. Cryopreservation of Plant Cell Lines Using Alginate Encapsulation
Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack
35. Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3
Angelika Senula and Manuela Nagel
36. Cryopreservation of Seeds and Seed Embryos in Orthodox, Intermediate, and Recalcitrant Seeded Species
Daniel Ballesteros, Natalia Fanega-Sleziak, and Rachael Davies
37. Freeze-Drying of Proteins
Baolin Liu and Xinli Zhou
38. Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical Properties
Fernanda Fonseca, Amélie Girardeau, and Stéphanie Passot
39. Preservation of Mammalian Sperm by Freeze-Drying
Levent Keskintepe and Ali Eroglu
40. Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf Availability
Willem F. Wolkers and Andres Hilfiker
Klappentext
This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
n
Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.nnThe chapter ¿Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation¿ is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.nn
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
