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Cryopreservation and Freeze-Drying Protocols
(Englisch)
Methods in Molecular Biology 2180
Wolkers, Willem F. & Oldenhof, Harriëtte

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Cryopreservation and Freeze-Drying Protocols

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Produktbeschreibung

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.

The chapter "Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.


Part I: Fundamental Aspects of Cryopreservation and Freeze-Drying

 

1. Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues

            Willem F. Wolkers and Harriëtte Oldenhof

 

2. Principles of Ice-Free Cryopreservation by Vitrification

            Gregory M. Fahy and Brian Wowk

 

3. The Principles of Freeze-Drying and Application of Analytical Technologies

            Kevin R. Ward and Paul Matejtschuk

 

4. Mathematical Modeling and Optimization of Cryopreservation in Single Cells

            James D. Benson

 

5. Mathematical Modeling of Protectant Transport in Tissues

            Ross M. Warner and Adam Z. Higgins

 

Part II: Technologies and Methods to Study Freezing and Drying

 

6. Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation

            Peter Kilbride and Julie Meneghel

 

7. Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of Biologics

            Shangping Wang, Susan Trammell, and Gloria D. Elliott

 

8. High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kinetics

            Jens O.M. Karlsson

 

9. Use of Ice Recrystallization Inhibition Assays to Screen for Compounds that Inhibit Ice Recrystallization

            Anna A. Ampaw, August Sibthorpe, and Robert N. Ben

 

10. DSC Analysis of Thermophysical Properties for Biomaterials and Formulations

            Wendell Q. Sun

 

11. Osmometric Measurements of Cryoprotective Agent Permeation into Tissues

            Kezhou Wu, Leila Laouar, Nadia Shardt, Janet A.W. Elliott, and Nadr M. Jomha

 

12. Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes

            Ariadna Corral, Alberto Olmo, and Ramón Risco

 

13. Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological Research

            Willem F. Wolkers and Harriëtte Oldenhof

 

14. Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements

            Guanglin Yu, Rui Li, and Allison Hubel

 

Part III: Cryopreservation and Freeze-Drying Protocols

 

15. Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm

            Harriëtte Oldenhof, Willem F. Wolkers, and Harald Sieme

 

16. Cryopreservation of Avian Semen

            Henri Woelders

 

17. Cryopreservation of Mouse Sperm for Genome Banking

            Yuksel Agca and Cansu Agca

 

18. Cryopreservation of Marine Invertebrates: From Sperm to Complex Larval Stages

            Estefania Paredes, Pablo Heres, Catarina Anjos, and Elsa Cabrita

 

19. Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into a Cooling Agent

            Mengying Wang, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, and Vladimir Isachenko

 

20. Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification as Successful Methods for Cryogenic Storage

            Victoria Keros and Barry J. Fuller

 

21. Vitrification of Porcine Oocytes and Zygotes in Microdrops on a Solid Metal Surface or Liquid Nitrogen

            Tamas Somfai and Kazuhiro Kikuchi

 

22. Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research

            Yuksel Agca and Cansu Agca

 

23. Cryopreservation and Thawing of Human Ovarian Cortex Tissue Slices

            Jana Liebenthron and Markus Montag

 

24. Vitrification: A Simple and Successful Method for Cryostorage of Human Blastocysts

            Juergen Liebermann

 

25. Vitrification of Equine In Vivo-Derived Embryos, after Blastocoel Aspiration

            Carolina Herrera

 

26. Frozen Blood Reserves

            Johan W. Lagerberg

 

27. Isolation, Cryopreservation, and Characterization of iPSC-Derived Megakaryocytes

            Denys Pogozhykh, Rainer Blasczyk, and Constança Figueiredo

 

28. Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem Cells

            Melany López and Ali Eroglu

 

29. Chemically-Defined and Xeno-Free Cryopreservation of Human Induced Pluripotent Stem Cells

            Juliette Seremak and Ali Eroglu

 

30. Protocol for Cryopreservation of Endothelial Monolayers

            Leah A. Marquez-Curtis, Nasim Eskandari, Locksley E. McGann, and Janet A.W. Elliott

 

31. Vitrification of Heart Valve Tissues

            Kelvin G.M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene, and Lia H. Campbell

 

32. Cryopreservation of Algae

            Estefania Paredes, Angela Ward, Ian Probert, Léna Gouhier, and Christine N. Campbell

 

33. Cryopreservation of Fern Spores and Pollen

            Anna Nebot, Victoria J. Philpott, Anna Pajdo, and Daniel Ballesteros

 

34. Cryopreservation of Plant Cell Lines Using Alginate Encapsulation

            Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack

 

35. Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3

            Angelika Senula and Manuela Nagel

 

36. Cryopreservation of Seeds and Seed Embryos in Orthodox, Intermediate, and Recalcitrant Seeded Species

            Daniel Ballesteros, Natalia Fanega-Sleziak, and Rachael Davies

 

37. Freeze-Drying of Proteins

            Baolin Liu and Xinli Zhou

 

38. Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical Properties

            Fernanda Fonseca, Amélie Girardeau, and Stéphanie Passot

 

39. Preservation of Mammalian Sperm by Freeze-Drying

            Levent Keskintepe and Ali Eroglu

 

40. Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf Availability

            Willem F. Wolkers and Andres Hilfiker


This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.

The chapter "Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.

The chapter "Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.


Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues.- Principles of Ice-Free Cryopreservation by Vitrification.- The Principles of Freeze-Drying and Application of Analytical Technologies.- Mathematical Modeling and Optimization of Cryopreservation in Single Cells.- Mathematical Modeling of Protectant Transport in Tissues.- Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation.- Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of Biologics.- High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kinetics.- Use of Ice Recrystallization Inhibition Assays to Screen for Compounds that Inhibit Ice Recrystallization.- DSC Analysis of Thermophysical Properties for Biomaterials and Formulations.- Osmometric Measurements of Cryoprotective Agent Permeation into Tissues.- Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes.- Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological Research.- Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements.- Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm.- Cryopreservation of Avian Semen.- Cryopreservation of Mouse Sperm for Genome Banking.- Cryopreservation of Marine Invertebrates: From Sperm to Complex Larval Stages.- Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into a Cooling Agent.- Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification as Successful Methods for Cryogenic Storage.- Vitrification of Porcine Oocytes and Zygotes in Microdrops on a Solid Metal Surface or Liquid Nitrogen.- Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research.- Cryopreservation and Thawing of Human Ovarian Cortex Tissue Slices.- Vitrification: A Simple and Successful Method for Cryostorage of Human Blastocysts.- Vitrificationof Equine In Vivo-Derived Embryos, after Blastocoel Aspiration.- Frozen Blood Reserves.- Isolation, Cryopreservation, and Characterization of iPSC-Derived Megakaryocytes.- Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem Cells.- Chemically-Defined and Xeno-Free Cryopreservation of Human Induced Pluripotent Stem Cells.- Protocol for Cryopreservation of Endothelial Monolayers.- Vitrification of Heart Valve Tissues.- Cryopreservation of Algae.- Cryopreservation of Fern Spores and Pollen.- Cryopreservation of Plant Cell Lines Using Alginate Encapsulation.- Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3.- Cryopreservation of Seeds and Seed Embryos in Orthodox, Intermediate, and Recalcitrant Seeded Species.- Freeze-Drying of Proteins.- Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical Properties.- Preservation of Mammalian Sperm by Freeze-Drying.- Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf Availability.



Inhaltsverzeichnis



Part I: Fundamental Aspects of Cryopreservation and Freeze-Drying

1. Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues

Willem F. Wolkers and Harriëtte Oldenhof

2. Principles of Ice-Free Cryopreservation by Vitrification

Gregory M. Fahy and Brian Wowk

3. The Principles of Freeze-Drying and Application of Analytical Technologies

Kevin R. Ward and Paul Matejtschuk

4. Mathematical Modeling and Optimization of Cryopreservation in Single Cells

James D. Benson

5. Mathematical Modeling of Protectant Transport in Tissues

Ross M. Warner and Adam Z. Higgins

Part II: Technologies and Methods to Study Freezing and Drying

6. Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation

Peter Kilbride and Julie Meneghel

7. Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of Biologics

Shangping Wang, Susan Trammell, and Gloria D. Elliott

8. High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kinetics

Jens O.M. Karlsson

9. Use of Ice Recrystallization Inhibition Assays to Screen for Compounds that Inhibit Ice Recrystallization

Anna A. Ampaw, August Sibthorpe, and Robert N. Ben

10. DSC Analysis of Thermophysical Properties for Biomaterials and Formulations

Wendell Q. Sun

11. Osmometric Measurements of Cryoprotective Agent Permeation into Tissues

Kezhou Wu, Leila Laouar, Nadia Shardt, Janet A.W. Elliott, and Nadr M. Jomha

12. Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes

Ariadna Corral, Alberto Olmo, and Ramón Risco

13. Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological Research

Willem F. Wolkers and Harriëtte Oldenhof

14. Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements

Guanglin Yu, Rui Li, and Allison Hubel

Part III: Cryopreservation and Freeze-Drying Protocols

15. Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm

Harriëtte Oldenhof, Willem F. Wolkers, and Harald Sieme

16. Cryopreservation of Avian Semen

Henri Woelders

17. Cryopreservation of Mouse Sperm for Genome Banking

Yuksel Agca and Cansu Agca

18. Cryopreservation of Marine Invertebrates: From Sperm to Complex Larval Stages

Estefania Paredes, Pablo Heres, Catarina Anjos, and Elsa Cabrita

19. Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into a Cooling Agent

Mengying Wang, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, and Vladimir Isachenko

20. Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification as Successful Methods for Cryogenic Storage

Victoria Keros and Barry J. Fuller

21. Vitrification of Porcine Oocytes and Zygotes in Microdrops on a Solid Metal Surface or Liquid Nitrogen

Tamas Somfai and Kazuhiro Kikuchi

22. Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research

Yuksel Agca and Cansu Agca

23. Cryopreservation and Thawing of Human Ovarian Cortex Tissue Slices

Jana Liebenthron and Markus Montag

24. Vitrification: A Simple and Successful Method for Cryostorage of Human Blastocysts

Juergen Liebermann

25. Vitrification of Equine In Vivo-Derived Embryos, after Blastocoel Aspiration

Carolina Herrera

26. Frozen Blood Reserves

Johan W. Lagerberg

27. Isolation, Cryopreservation, and Characterization of iPSC-Derived Megakaryocytes

Denys Pogozhykh, Rainer Blasczyk, and Constança Figueiredo

28. Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem Cells

Melany López and Ali Eroglu

29. Chemically-Defined and Xeno-Free Cryopreservation of Human Induced Pluripotent Stem Cells

Juliette Seremak and Ali Eroglu

30. Protocol for Cryopreservation of Endothelial Monolayers

Leah A. Marquez-Curtis, Nasim Eskandari, Locksley E. McGann, and Janet A.W. Elliott

31. Vitrification of Heart Valve Tissues

Kelvin G.M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene, and Lia H. Campbell

32. Cryopreservation of Algae

Estefania Paredes, Angela Ward, Ian Probert, Léna Gouhier, and Christine N. Campbell

33. Cryopreservation of Fern Spores and Pollen

Anna Nebot, Victoria J. Philpott, Anna Pajdo, and Daniel Ballesteros

34. Cryopreservation of Plant Cell Lines Using Alginate Encapsulation

Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack

35. Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3

Angelika Senula and Manuela Nagel

36. Cryopreservation of Seeds and Seed Embryos in Orthodox, Intermediate, and Recalcitrant Seeded Species

Daniel Ballesteros, Natalia Fanega-Sleziak, and Rachael Davies

37. Freeze-Drying of Proteins

Baolin Liu and Xinli Zhou

38. Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical Properties

Fernanda Fonseca, Amélie Girardeau, and Stéphanie Passot

39. Preservation of Mammalian Sperm by Freeze-Drying

Levent Keskintepe and Ali Eroglu

40. Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf Availability

Willem F. Wolkers and Andres Hilfiker


Klappentext

This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

n
Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.nnThe chapter ¿Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation¿ is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.nn




Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts



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