-Provides instructions for handling of different plant model systems -Offers cutting-edge methods for analyzing plant development on the phenotypic, molecular and biochemical level-Includes in-depth explanations and troubleshooting advice for all methods
Provides instructions for handling of different plant model systems
Offers cutting-edge methods for analyzing plant development on the phenotypic, molecular and biochemical level
Includes in-depth explanations and troubleshooting advice for all methods
Includes supplementary material: sn.pub/extras
Plants come in myriads of shapes and colors, and the beauty of plants has fascinated mankind for thousands of years. Long before Mendel discovered the laws of heritab- ity and Darwin developed his theory on evolution, the affection for ornamental plants led people to select alleles that establish novel plant forms. Today, plant developmental biology tries to discover the mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant´s life. Although the underlying processes of cell proliferation and differentiation are similar in plants and a- mals, plants are different because their development is usually open, and its outcome is not the faithful repetition of a general plan but is strongly in?uenced by environm- tal conditions. In the last few decades, plant developmental biology has pinpointed a large number of developmental regulators and their interactions and the mechanisms that govern plant development start to emerge. In part, this progress was enabled by the advance of powerful molecular tools for a few model species, most importantly Arabidopsis. This volume of the Methods in Molecular Biology series provides a collection of protocols for many of the common experimental approaches in plant developmental bi- ogy. All chapters are written in the same format as that used in the Methods in Molecular TM Biology series. Each chapter opens with a deillegalscription of the basic theory behind the method being described.
Plant Developmental BiologyEdited by Lars Hennig and Claudia KöhlerContents1. Growth protocols for model plants in developmental biologyLars Hennig2. Grafting as a research toolColin G. N. Turnbull3. Viral-induced gene silencing as a reverse genetics tool to study gene functionSteven Bernacki, Mansour Karimi, Pierre Hilson and Niki Robertson4. The CRE/lox system as a tool for developmental studies at the cell and tissue levelGuy Wachsman and Renze Heidstra5. Inducible gene expression systems for plantsLorenzo Borghi6. Trichome development in ArabidopsisJoachim F. Uhrig and Martin Hülskamp7. Phenotyping the development of leaf area in Arabidopsis thalianaSarah J. Cookson, Olivier Turc, Catherine Massonnet and Christine Granier8. Analyzing shoot apical meristem developmentCristel Carles, Chan Man Ha, Ji Hyung Jun, Elisa Fiume and Jennifer C. Fletcher9. Analyzing floral meristem developmentElisa Fiume, Helena R. Pires, Jin Sun Kim and Jennifer C. Fletcher10. Female gametophytic mutants: Diagnosis and characterizationRonny Völz and Rita Groß-Hardt11. Pollen tube developmentMark A. Johnson and Benedikt Kost12. Analysis of root meristem size developmentSerena Perilli and Sabrina Sabatini13. Phenotypic characterization of photomorphogenic responses during plant development Thomas Kretsch14. Kinematic analysis of cell division and expansionBart Rymen, Frederik Coppens, Stijn Dhondt, Fabio Fiorani and Gerrit T.S. Beemster15. Flowering time controlYvonne Möller-Steinbach, Cristina Alexandre and Lars Hennig16. mRNA detection by whole mount in situ hybridization WISH or sectioned tissue in situ hybridzation SISH in ArabidopsisYvonne Stahl and Rüdiger Simon17. Immunolocalization of proteins in plantsMichael Sauer and Ji?í Friml 18. Detection of small non-coding RNAsTamas Dalmay19. Quantitative real time PCR in plant developmental biologyVivien Exner20. Luciferase and green fluorescent protein reporter genes as tools todetermine protein abundance and intracellular dynamicsAndrás Viczián and Stefan Kircher21. Fluorescence-activated cell sorting in plant developmental biologyAnjali S. Iyer-Pascuzzi and Philip N. Benfey22. Laser microdissection of paraffin embedded plant tissues for tranillegalscript profilingRobert C. Day23. Utilizing bimolecular fluorescence complementation BiFC to assay protein-protein interaction in plantsNir Ohad and Shaul Yalovsky24. The split luciferase complementation assayNaohiro Kato and Jason Jones25. Co-immunoprecipitation and protein blotsErika Isono and Claus Schwechheimer26. Probing protein-protein interactions with FRET-FLIMChristoph Bücherl, José Aker, Sacco de Vries, Jan Willem Borst27. Plant chromatin immunoprecipitationCorina B. R. Villar and Claudia Köhler28. Immunocytological analysis of chromatin in isolated nucleiPenka Pavlova, Federico Tessadori, Hans J. de Jong and Paul Fransz29. Bisulphite sequencing of plant genomic DNAErnst Aichinger and Claudia Köhler
The beauty and variety of plants has fascinated mankind for thousands of years, and this fascination continues today as plant developmental biologists discover mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant´s life. In Plant Developmental Biology: Methods and Protocols, expert researchers provide a collection of protocols for many of the common experimental approaches in plant developmental biology, including diverse methods that range from grafting over bimolecular fluorescence complementation to chromatin immunoprecipitation. Chapters are divided into six major parts: growth protocols, manipulation of gene activity, assaying developmental phenotypes, assaying gene activity, testing protein-protein interactions, and probing chromatin. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and revolutionary, Plant Developmental Biology: Methods and Protocols is an essential guide for all plant developmental geneticists, biochemists, and researchers in stress physiology and plant nutrition.
Plants come in myriads of shapes and colors, and the beauty of plants has fascinated mankind for thousands of years. In the last few decades, plant developmental biology has pinpointed a large number of developmental regulators and their interactions and the mechanisms that govern plant development start to emerge.
The beauty and variety of plants has fascinated mankind for thousands of years. In this comprehensive and revolutionary book, expert researchers provide a collection of protocols for many of the common experimental approaches in plant developmental biology.
Plants come in myriads of shapes and colors, and the beauty of plants has fascinated mankind for thousands of years. Long before Mendel discovered the laws of heritab- ity and Darwin developed his theory on evolution, the affection for ornamental plants led people to select alleles that establish novel plant forms. Today, plant developmental biology tries to discover the mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant's life. Although the underlying processes of cell proliferation and differentiation are similar in plants and a- mals, plants are different because their development is usually open, and its outcome is not the faithful repetition of a general plan but is strongly in?uenced by environm- tal conditions. In the last few decades, plant developmental biology has pinpointed a large number of developmental regulators and their interactions and the mechanisms that govern plant development start to emerge. In part, this progress was enabled by the advance of powerful molecular tools for a few model species, most importantly Arabidopsis. This volume of the Methods in Molecular Biology series provides a collection of protocols for many of the common experimental approaches in plant developmental bi- ogy. All chapters are written in the same format as that used in the Methods in Molecular TM Biology series. Each chapter opens with a deillegalscription of the basic theory behind the method being described.
Growth Protocols for Model Plants in Developmental Biology.- Grafting as a Research Tool.- Virus-Induced Gene Silencing as a Reverse Genetics Tool to Study Gene Function.- The CRE/lox System as a Tool for Developmental Studies at the Cell and Tissue Level.- Inducible Gene Expression Systems for Plants.- Trichome Development in Arabidopsis.- Phenotyping the Development of Leaf Area in Arabidopsis thaliana.- Analyzing Shoot Apical Meristem Development.- Analyzing Floral Meristem Development.- Female Gametophytic Mutants: Diagnosis and Characterization.- Pollen Tube Development.- Analysis of Root Meristem Size Development.- Phenotypic Characterization of Photomorphogenic Responses During Plant Development.- Kinematic Analysis of Cell Division and Expansion.- Flowering Time Control.- mRNA Detection by Whole Mount In Situ Hybridization (WISH) or Sectioned Tissue In Situ Hybridization (SISH) in Arabidopsis.- Immunolocalization of Proteins in Plants.- Detection of Small Non-coding RNAs.- Quantitative Real Time PCR in Plant Developmental Biology.- Luciferase and Green Fluorescent Protein Reporter Genes as Tools to Determine Protein Abundance and Intracellular Dynamics.- Fluorescence-Activated Cell Sorting in Plant Developmental Biology.- Laser Microdissection of Paraffin-Embedded Plant Tissues for Tranillegalscript Profiling.- Utilizing Bimolecular Fluorescence Complementation (BiFC) to Assay Protein-Protein Interaction in Plants.- The Split Luciferase Complementation Assay.- Co-immunoprecipitation and Protein Blots.- Probing Protein-Protein Interactions with FRET-FLIM.- Plant Chromatin Immunoprecipitation.- Immunocytological Analysis of Chromatin in Isolated Nuclei.- Bisulphite Sequencing of Plant Genomic DNA.
Inhaltsverzeichnis
Plant Developmental Biology
Edited by Lars Hennig and Claudia Köhler
Contents
1. Growth protocols for model plants in developmental biology
Lars Hennig
2. Grafting as a research tool
Colin G. N. Turnbull
3. Viral-induced gene silencing as a reverse genetics tool to study gene function
Steven Bernacki, Mansour Karimi, Pierre Hilson and Niki Robertson
4. The CRE/lox system as a tool for developmental studies at the cell and tissue level
Guy Wachsman and Renze Heidstra
5. Inducible gene expression systems for plants
Lorenzo Borghi
6. Trichome development in Arabidopsis
Joachim F. Uhrig and Martin Hülskamp
7. Phenotyping the development of leaf area in Arabidopsis thaliana
Sarah J. Cookson, Olivier Turc, Catherine Massonnet and Christine Granier
8. Analyzing shoot apical meristem development
Cristel Carles, Chan Man Ha, Ji Hyung Jun, Elisa Fiume and Jennifer C. Fletcher
9. Analyzing floral meristem development
Elisa Fiume, Helena R. Pires, Jin Sun Kim and Jennifer C. Fletcher
10. Female gametophytic mutants: Diagnosis and characterization
Ronny Völz and Rita Groß-Hardt
11. Pollen tube development
Mark A. Johnson and Benedikt Kost
12. Analysis of root meristem size development
Serena Perilli and Sabrina Sabatini
13. Phenotypic characterization of photomorphogenic responses during plant development
Thomas Kretsch
14. Kinematic analysis of cell division and expansion
Bart Rymen, Frederik Coppens, Stijn Dhondt, Fabio Fiorani and Gerrit T.S. Beemster
15. Flowering time control
Yvonne Möller-Steinbach, Cristina Alexandre and Lars Hennig
16. mRNA detection by whole mount in situ hybridization WISH or sectioned tissue in situ hybridzation SISH in Arabidopsis
Yvonne Stahl and Rüdiger Simon
17. Immunolocalization of proteins in plants
Michael Sauer and Ji?í Friml
18. Detection of small non-coding RNAs
Tamas Dalmay
19. Quantitative real time PCR in plant developmental biology
Vivien Exner
20. Luciferase and green fluorescent protein reporter genes as tools todetermine protein abundance and intracellular dynamics
András Viczián and Stefan Kircher
21. Fluorescence-activated cell sorting in plant developmental biology
Anjali S. Iyer-Pascuzzi and Philip N. Benfey
22. Laser microdissection of paraffin embedded plant tissues for tranillegalscript profiling
Robert C. Day
23. Utilizing bimolecular fluorescence complementation BiFC to assay protein-protein interaction in plants
Nir Ohad and Shaul Yalovsky
24. The split luciferase complementation assay
Naohiro Kato and Jason Jones
25. Co-immunoprecipitation and protein blots
Erika Isono and Claus Schwechheimer
26. Probing protein-protein interactions with FRET-FLIM
Christoph Bücherl, José Aker, Sacco de Vries, Jan Willem Borst
27. Plant chromatin immunoprecipitation
Corina B. R. Villar and Claudia Köhler
28. Immunocytological analysis of chromatin in isolated nuclei
Penka Pavlova, Federico Tessadori, Hans J. de Jong and Paul Fransz
29. Bisulphite sequencing of plant genomic DNA
Ernst Aichinger and Claudia Köhler
Klappentext
Plants come in myriads of shapes and colors, and the beauty of plants has fascinated mankind for thousands of years. Long before Mendel discovered the laws of heritab- ity and Darwin developed his theory on evolution, the affection for ornamental plants led people to select alleles that establish novel plant forms. Today, plant developmental biology tries to discover the mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant¿s life. Although the underlying processes of cell proliferation and differentiation are similar in plants and a- mals, plants are different because their development is usually open, and its outcome is not the faithful repetition of a general plan but is strongly in?uenced by environm- tal conditions. In the last few decades, plant developmental biology has pinpointed a large number of developmental regulators and their interactions and the mechanisms that govern plant development start to emerge. In part, this progress was enabled by the advance of powerful molecular tools for a few model species, most importantly Arabidopsis. This volume of the Methods in Molecular Biology series provides a collection of protocols for many of the common experimental approaches in plant developmental bi- ogy. All chapters are written in the same format as that used in the Methods in Molecular TM Biology series. Each chapter opens with a deillegalscription of the basic theory behind the method being described.
Provides instructions for handling of different plant model systems
rn
Offers cutting-edge methods for analyzing plant development on the phenotypic, molecular and biochemical level
rn
Includes in-depth explanations and troubleshooting advice for all methods
rn
Includes supplementary material: sn.pub/extras
rn
