Presents selected genetic, molecular, and cellular techniques used in somatic stem cell reserach and its clinical application
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Stem cells are found in almost all organisms from the early stages of development to the end of life. There are several types of stem cells and all of them may prove useful for medical research; however, each of the different types has both promise and limitations. Somatic Stem Cells: Methods and Protocols presents selected genetic, molecular, and cellular techniques used in somatic stem cell research and its clinical application. Chapters focus on the isolation, characterization, purity, plasticity, and clinical uses of somatic stem cells from a variety of human and animal tissues. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Through and intuitive, Somatic Stem Cells: Methods and Protocols seeks to provides scientists with the fundamental techniques of stem cell research and its potential application in regenerative medicine.
Section I: Introduction
1. Current Thoughts on the Therapeutic Potential of Stem Cell
Pranela Rameshwar
Section II: Stem Cell Study in Lower Organism
2. A Unique FACS Method to Isolate Stem Cells in Planarian
Tetsutaro Hayashi and Kiyokazu Agata
3. Identification of Neural Stem Cells in the Drosophila Larval Brain
Mo Weng, Hideyuki Komori, and Cheng-Yu Lee
4. Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut
Shree Ram Singh, Manoj K. Mishra, Steven X. Hou, and Maduri Kango-Singh
Section III: Stem Cell Study in Murine and Human Model
5. Developing a Quantitative In Vivo Tissue Reconstitution Assay to Assess the Relative Potency of Candidate Populations of Mouse Oesophageal Epithelial Cells
Daniel Croagh, Rick Redvers, Wayne A. Phillips, and Pritinder Kaur
6. Identification, Isolation, and Culture of Intestinal Epithelial Stem Cells from Murine Intestine
Gracz, A.D., Puthoff, B.J., Magness, S.T.
7. Isolation and Characterization of Distal Lung Progenitor Cells
Barbara Driscoll, Alex Kikuchi, Allison N. Lau, Jooeun Lee, Raghava Reddy, Edwin Jesudason, Carla F. Kim,?David Warburton
8. Transplantation of Mouse Fetal Liver Cells for Analyzing the Function of Hematopoietic Stem and Progenitor Cells
Kristbjorn Orri Gudmundsson, Steven W. Stull, and Jonathan R. Keller
9. Convenient and Efficient Enrichment of the CD133+ Liver Cells From Rat Fetal Liver as a Source of Liver Stem/Progenitor Cells
Weihui Liu, Nan You, Kefeng Dou
10. Assessing the Potential Clinical Utility of Transplantations of Neural and Mesenchymal Stem Cells for Treating Neurodegenerative Diseases
Laurent Lescaudron, C.Boyer, Virginie Bonnamain, KD. Fink, X. Lévêque, J. Rossignol, V. Nerrière-Daguin, AC Malouet, F. Lelan, N.D Dey, D. Michel-Monigadon, M. Lu, I. Neveu, S. von Hörsten, P. Naveilhan and GL Dunbar
11. Functional Identification of Neural Stem Cell-Derived Oligodendrocytes
Sofia Grade, Fabienne Agasse, Liliana Bernardino, João O. Malva
12. Stem/Progenitor Cells in Murine Mammary Gland: Isolation and Functional Characterization
Abhik Bandyopadhyay, Qiaoxiang Dong and Lu-Zhe Sun
13. A Reporter Assay to Detect Transfer and Targeting of miRNAs in Stem Cell-Breast Cancer Co-Cultures
Steven J. Greco, Shyam A. Patel, Pranela Rameshwar
14. Isolation, Culture and Osteogenic/chondrogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells
Susanne Grässel, Sabine Stöckl, Zsuzsa Jenei-Lanzl
15. Obtaining Freshly Isolated and Cultured Mesenchymal Stem Cells from Human Adipose Tissue
Andrew C. Boquest and Philippe Collas
16. Collection, Processing and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regenerative Medicine
Michael S. Badowski and David T. Harris
17. Generation of Functional Islets from Human Umbilical Cord and Placenta Derived Mesenchymal Stem Cells
Sachin Kadam, Vijayendran Govindasamy, and Ramesh Bhonde
18. Isolation and Characterization of Human Prostate Stem/Progenitor Cells
Changyong Guo, Baohui Zhang, and Isla P. Garraway
19. Isolation and Expansion of Adult Cardiac Stem/Progenitor Cells in the Form of Cardiospheres from Human Cardiac Biopsies and Murine Hearts
Isotta Chimenti, Roberto Gaetani, Lucio Barile, Elvira Forte, Vittoria Ionta, Francesco Angelini, Giacomo Frati, Elisa Messina, Alessandro Giacomello.
20. Isolation and Differentiation of Human Cardiomyocyte Progenitor Cells Into Cardiomyocytes
Anke M. Smits, Angelique A. van Oorschot and Marie-José Goumans
21. Isolation, Expansion and Characterization of Human Islet-Derived Progenitor Cells
Mugdha V. Joglekar and Anandwardhan A. Hardikar
22. Isolation and Characterization of Resident Mesenchymal Stem Cells in Human Glomeruli
Stefania Bruno and Giovanni Camussi
23. Endothelial Colony Forming Progenitor Cell Isolation and Expansion
Nicole A. Hofmann, Andreas Reinisch and Dirk Strunk
24. Isolation and Characterization of Stem Cell-Enriched Human and Canine Hair Follicle Keratinocytes
Manabu Ohyama and Tetsuro Kobayashi
25. Human Salivary Gland Stem Cells: Isolation, Propagation and Characterization
Silke Schwarz and Nicole Rotter
26. Identification, Isolation, Characterization and Banking of Human Dental Pulp Stem Cells
Virginia Tirino, Francesca Paino and Gianpaolo Papaccio
27. Isolation and Differentiation Potential of Fibroblast-Like Stromal Cells Derived from Human Skin
Hsing-I Huang and Chung-Zu Wu
28. Immortalization of Human Mesenchymal Stromal Cells with Telomerase and Red Fluorescence Protein Expression
Chao-Ling Yao and Shiaw-Min Hwang
29. Genetic Modification of Mesenchymal Stem Cells
Andréia Escosteguy Vargas, Melissa Medeiros Markoski, Andrés Delgado Cañedo, Flávia Helena da Silva and Nance Beyer Nardi
30. Methodology, Biology and Clinical Applications of Human Mesenchymal Stem Cells
Melissa Camassola, Luisa Maria Gomes de Macedo Braga, Pedro Cesar Chagastelles and Nance Beyer Nardi
31. In Vitro Production of Enucleated Red Blood Cells from Hematopoietic Stem and Progenitor Cells
Kenichi Miharada and Yukio Nakamura
32. Methods for Cancer Stem Cell Detection and Isolation
Virginia Tirino, Vincenzo Desiderio, Francesca Paino and Gianpaolo Papaccio
33. Biocompatible Nanoparticle Labelling of Stem Cells and their Distribution in Brain
Ashish K. Rehni, Thakur Gurjeet Singh, Mansi Chitkara, and I. S. Sandhu
This Methods in Molecular Biology(TM) volume offers selected genetic, molecular, and cellular techniques used in somatic stem cell research and its clinical application. Includes materials and reagent lists, step-by-step protocols, troubleshooting advice and more.
Stem cells are found in almost all organisms from the early stages of development to the end of life. There are several types of stem cells and all of them may prove useful for medical research; however, each of the different types has both promise and limitations. Somatic Stem Cells: Methods and Protocols presents selected genetic, molecular, and cellular techniques used in somatic stem cell research and its clinical application. Chapters focus on the isolation, characterization, purity, plasticity, and clinical uses of somatic stem cells from a variety of human and animal tissues. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Through and intuitive, Somatic Stem Cells: Methods and Protocols seeks to provides scientists with the fundamental techniques of stem cell research and its potential application in regenerative medicine.
Current Thoughts on the Therapeutic Potential of Stem Cell.-A Unique FACS Method to Isolate Stem Cells in Planarian.-Identification of Neural Stem Cells in the Drosophila Larval Brain.-Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut.-Developing a Quantitative In Vivo Tissue Reconstitution Assay to Assess the Relative Potency of Candidate Populations of Mouse Oesophageal Epithelial Cells.-Identification, Isolation, and Culture of Intestinal Epithelial Stem Cells from Murine Intestine.-Isolation and Characterization of Distal Lung Progenitor Cells.-Transplantation of Mouse Fetal Liver Cells for Analyzing the Function of Hematopoietic Stem and Progenitor Cells.-Convenient and Efficient Enrichment of the CD133+ Liver Cells From Rat Fetal Liver as a Source of Liver Stem/Progenitor Cells.-Assessing the Potential Clinical Utility of Transplantations of Neural and Mesenchymal Stem Cells for Treating Neurodegenerative Diseases.-Functional Identification of Neural Stem Cell-Derived Oligodendrocytes.-Stem/Progenitor Cells in Murine Mammary Gland: Isolation and Functional Characterization.-A Reporter Assay to Detect Transfer and Targeting of miRNAs in Stem Cell-Breast Cancer Co-Cultures.-Isolation, Culture and Osteogenic/chondrogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells.-Obtaining Freshly Isolated and Cultured Mesenchymal Stem Cells from Human Adipose Tissue.-Collection, Processing and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regenerative Medicine.-Generation of Functional Islets from Human Umbilical Cord and Placenta Derived Mesenchymal Stem Cells.-Isolation and Characterization of Human Prostate Stem/Progenitor Cells.-Isolation and Expansion of Adult Cardiac Stem/Progenitor Cells in the Form of Cardiospheres from Human Cardiac Biopsies and Murine Hearts.-Isolation and Differentiation of Human Cardiomyocyte Progenitor Cells Into Cardiomyocytes.-Isolation, Expansion andCharacterization of Human Islet-Derived Progenitor Cells.-Isolation and Characterization of Resident Mesenchymal Stem Cells in Human Glomeruli.-Endothelial Colony Forming Progenitor Cell Isolation and Expansion.-Isolation and Characterization of Stem Cell-Enriched Human and Canine Hair Follicle Keratinocytes.-Human Salivary Gland Stem Cells: Isolation, Propagation and Characterization.-Identification, Isolation, Characterization and Banking of Human Dental Pulp Stem Cells.-Isolation and Differentiation Potential of Fibroblast-Like Stromal Cells Derived from Human Skin.-Immortalization of Human Mesenchymal Stromal Cells with Telomerase and Red Fluorescence Protein Expression.-Genetic Modification of Mesenchymal Stem Cells.-Methodology, Biology and Clinical Applications of Human Mesenchymal Stem Cells.-In Vitro Production of Enucleated Red Blood Cells from Hematopoietic Stem and Progenitor Cells.-Methods for Cancer Stem Cell Detection and Isolation.-Biocompatible Nanoparticle Labelling of Stem Cells and their Distribution in Brain.
Inhaltsverzeichnis
Section I: Introduction1. Current Thoughts on the Therapeutic Potential of Stem CellPranela RameshwarSection II: Stem Cell Study in Lower Organism 2. A Unique FACS Method to Isolate Stem Cells in PlanarianTetsutaro Hayashi and Kiyokazu Agata 3. Identification of Neural Stem Cells in the Drosophila Larval BrainMo Weng, Hideyuki Komori, and Cheng-Yu Lee4. Generation and Staining of Intestinal Stem Cell Lineage in Adult MidgutShree Ram Singh, Manoj K. Mishra, Steven X. Hou, and Maduri Kango-Singh Section III: Stem Cell Study in Murine and Human Model5. Developing a Quantitative In Vivo Tissue Reconstitution Assay to Assess the Relative Potency of Candidate Populations of Mouse Oesophageal Epithelial CellsDaniel Croagh, Rick Redvers, Wayne A. Phillips, and Pritinder Kaur6. Identification, Isolation, and Culture of Intestinal Epithelial Stem Cells from Murine IntestineGracz, A.D., Puthoff, B.J., Magness, S.T. 7. Isolation and Characterization of Distal Lung Progenitor CellsBarbara Driscoll, Alex Kikuchi, Allison N. Lau, Jooeun Lee, Raghava Reddy, Edwin Jesudason, Carla F. Kim,¿David Warburton 8. Transplantation of Mouse Fetal Liver Cells for Analyzing the Function of Hematopoietic Stem and Progenitor CellsKristbjorn Orri Gudmundsson, Steven W. Stull, and Jonathan R. Keller9. Convenient and Efficient Enrichment of the CD133+ Liver Cells From Rat Fetal Liver as a Source of Liver Stem/Progenitor CellsWeihui Liu, Nan You, Kefeng Dou 10. Assessing the Potential Clinical Utility of Transplantations of Neural and Mesenchymal Stem Cells for Treating Neurodegenerative Diseases Laurent Lescaudron, C.Boyer, Virginie Bonnamain, KD. Fink, X. Lévêque, J. Rossignol, V. Nerrière-Daguin, AC Malouet, F. Lelan, N.D Dey, D. Michel-Monigadon, M. Lu, I. Neveu, S. von Hörsten, P. Naveilhan and GL Dunbar 11. Functional Identification of Neural Stem Cell-Derived OligodendrocytesSofia Grade, Fabienne Agasse, Liliana Bernardino, João O. Malva12. Stem/Progenitor Cells in Murine Mammary Gland: Isolation and Functional CharacterizationAbhik Bandyopadhyay, Qiaoxiang Dong and Lu-Zhe Sun 13. A Reporter Assay to Detect Transfer and Targeting of miRNAs in Stem Cell-Breast Cancer Co-Cultures Steven J. Greco, Shyam A. Patel, Pranela Rameshwar 14. Isolation, Culture and Osteogenic/chondrogenic Differentiation of Bone Marrow Derived Mesenchymal Stem CellsSusanne Grässel, Sabine Stöckl, Zsuzsa Jenei-Lanzl 15. Obtaining Freshly Isolated and Cultured Mesenchymal Stem Cells from Human Adipose TissueAndrew C. Boquest and Philippe Collas 16. Collection, Processing and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regenerative Medicine Michael S. Badowski and David T. Harris17. Generation of Functional Islets from Human Umbilical Cord and Placenta Derived Mesenchymal Stem Cells Sachin Kadam, Vijayendran Govindasamy, and Ramesh Bhonde 18. Isolation and Characterization of Human Prostate Stem/Progenitor CellsChangyong Guo, Baohui Zhang, and Isla P. Garraway 19. Isolation and Expansion of Adult Cardiac Stem/Progenitor Cells in the Form of Cardiospheres from Human Cardiac Biopsies and Murine Hearts Isotta Chimenti, Roberto Gaetani, Lucio Barile, Elvira Forte, Vittoria Ionta, Francesco Angelini, Giacomo Frati, Elisa Messina, Alessandro Giacomello. 20. Isolation and Differentiation of Human Cardiomyocyte Progenitor Cells Into CardiomyocytesAnke M. Smits, Angelique A. van Oorschot and Marie-José Goumans 21. Isolation, Expansion and Characterization of Human Islet-Derived Progenitor CellsMugdha V. Joglekar and Anandwardhan A. Hardikar 22. Isolation and Characterization of Resident Mesenchymal Stem Cells in Human GlomeruliStefania Bruno and Giovanni Camussi 23. Endothelial Colony Forming Progenitor Cell Isolation and ExpansionNicole A. Hofmann, Andreas Reinisch and Dirk Strunk 24. Isolation and Characterization of Stem Cell-Enriched Human and Canine Hair Follicle KeratinocytesManabu Ohyama and Tetsuro Kobayashi 25. Human Salivary Gland Stem Cells: Isolation, Propagation and CharacterizationSilke Schwarz and Nicole Rotter 26. Identification, Isolation, Characterization and Banking of Human Dental Pulp Stem CellsVirginia Tirino, Francesca Paino and Gianpaolo Papaccio 27. Isolation and Differentiation Potential of Fibroblast-Like Stromal Cells Derived from Human SkinHsing-I Huang and Chung-Zu Wu 28. Immortalization of Human Mesenchymal Stromal Cells with Telomerase and Red Fluorescence Protein ExpressionChao-Ling Yao and Shiaw-Min Hwang 29. Genetic Modification of Mesenchymal Stem CellsAndréia Escosteguy Vargas, Melissa Medeiros Markoski, Andrés Delgado Cañedo, Flávia Helena da Silva and Nance Beyer Nardi30. Methodology, Biology and Clinical Applications of Human Mesenchymal Stem CellsMelissa Camassola, Luisa Maria Gomes de Macedo Braga, Pedro Cesar Chagastelles and Nance Beyer Nardi 31. In Vitro Production of Enucleated Red Blood Cells from Hematopoietic Stem and Progenitor CellsKenichi Miharada and Yukio Nakamura32. Methods for Cancer Stem Cell Detection and IsolationVirginia Tirino, Vincenzo Desiderio, Francesca Paino and Gianpaolo Papaccio 33. Biocompatible Nanoparticle Labelling of Stem Cells and their Distribution in BrainAshish K. Rehni, Thakur Gurjeet Singh, Mansi Chitkara, and I. S. Sandhu
Klappentext
Stem cells are found in almost all organisms from the early stages of development to the end of life. There are several types of stem cells and all of them may prove useful for medical research; however, each of the different types has both promise and limitations. Somatic Stem Cells: Methods and Protocols presents selected genetic, molecular, and cellular techniques used in somatic stem cell research and its clinical application. Chapters focus on the isolation, characterization, purity, plasticity, and clinical uses of somatic stem cells from a variety of human and animal tissues. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.n nThrough and intuitive, Somatic Stem Cells: Methods and Protocols seeks to provides scientists with the fundamental techniques of stem cell research and its potential application in regenerative medicine.
Presents selected genetic, molecular, and cellular techniques used in somatic stem cell reserach and its clinical application
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
