Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
This volume aims to present a large panel of techniques for the study of Plant Cell Division. Plant Cell Division: Methods and Protocols captures basic experimental protocols that are commonly used to study plant cell division processes, as well as more innovative procedures. Chapters are split into five parts covering several different aspect of plant cell division such as, cell cultures for cell division studies, cell cycle progression and mitosis, imaging plant cell division, cell division and morphogenesis, and cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Cell Division: Methods and Protocols is a valuable tool for the study of plant cell division at both the cellular and molecular levels, and in the context of plant development.
Part I: Cell Cultures for Cell Division Studies
1. Synchronization Of Green Algae By Light And Dark Regimes For Cell Cycle and Cell Division Studies
Monika Hlavová, Milada Vítová, and Katerina Bišová
2. Plant Cell Division Analyzed by Transient Agrobacterium-Mediated Transformation of Tobacco BY-2 Cells
Henrik Buschmann
Part II: Cell Cycle and Mitosis
3. Identifying Gene Regulatory Networks In Arabidopsis By In Silico Prediction, Yeast-1-Hybrid and Inducible Gene Profiling Assays
Erin E. Sparks and Philip N. Benfey
4. Cytrap Marker Systems for In Vivo Visualization of Cell Cycle Progression in Arabidopsis
Shiori Sugamata Aki and Masaaki Umeda
5. Imaging Nuclear Morphology and Organization in Cleared Plant Tissues Treated with Cell Cycle Inhibitors
José Dijair Antonino De Souza Junior, Maria Fatima Grossi De Sa, Gilbert Engler and, Janice De Almeida Engler
6. Isolation of Plant Nuclei at Defined Cell Cycle Stages Using Edu Labeling and Flow Cytometry
Emily E. Wear, Lorenzo Concia, Ashley M. Brooks, Emily A. Markham, Tae-Jin Lee, George C. Allen, William F. Thompson, and Linda Hanley-Bowdoin
7. Preparation and Fluorescent Analysis of Plant Metaphase Chromosomes
Trude Schwarzacher
8. Fluorescence In Situ Hybridization On Rice Chromosomes
Yafei Li and Zhukuan Cheng
Part III Imaging Plant Cell Division
9. Dynamics of the Plant Nuclear Envelope During Cell Division
David E Evans and Katja Graumann
10. Immunolabeling Of Nuclei/Chromosomes In Arabidopsis Thaliana
Michael Sandmann, Joerg Fuchs, and Inna Lermontova
11. In Vivo Imaging Of Microtubules Organization In Dividing Giant Cell
Marie-Cecile Caillaud and Bruno Favery
Part IV: Cell Division and Morphogenesis
12. Real-Time Lineage Analysis to Study Cell Division Orientation in the Arabidopsis Shoot Meristem
Cory J. Tobin and Elliot M. Meyerowitz
13. Cell Proliferation Analysis Using Edu Labeling In Whole Plant and Histological Samples Of Arabidopsis
Anita Kazda, Svetlana Akimcheva, J. Matthew Watson, and Karel Riha
14. Studying Cell Division Plane Positioning in Early-Stage Embryos
Katia Belcram, Jean-Christophe Palauqui, and Martine Pastuglia
Part V: Cytokinesis
15. Characterization Oof Cytokinetic Mutants using Small Fluorescent Probes
Andrei Smertenko, Panagiotis Moschou, Laining Zhang, Deirdre Fahy, and Peter Bozhkov
16. Using Live Cell Markers in Maize to Analyze Cell Division Orientation and Timing Carolyn G. Rasmussen
17. Ratiometric Fluorescence Live Imaging Analysis of Membrane Lipid Order in Arabidopsis Mitotic Cells Using a Lipid Order-Sensitive Probe
Patricia Gerbeau-Pissot, Christophe Der, Markus Grebe, and Thomas Stanislas
This volume aims to present a large panel of techniques for the study of Plant Cell Division. Plant Cell Division: Methods and Protocols captures basic experimental protocols that are commonly used to study plant cell division processes, as well as more innovative procedures. Chapters are split into five parts covering several different aspect of plant cell division such as, cell cultures for cell division studies, cell cycle progression and mitosis, imaging plant cell division, cell division and morphogenesis, and cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Cell Division: Methods and Protocols is a valuable tool for the study of plant cell division at both the cellular and molecular levels, and in the context of plant development.
Synchronization Of Green Algae By Light And Dark Regimes For Cell Cycle and Cell Division Studies.- Plant Cell Division Analyzed by Transient Agrobacterium-Mediated Transformation of Tobacco BY-2 Cells.- Identifying Gene Regulatory Networks In Arabidopsis By In Silico Prediction, Yeast-1-Hybrid and Inducible Gene Profiling Assays.- Cytrap Marker Systems for In Vivo Visualization of Cell Cycle Progression in Arabidopsis.- Imaging Nuclear Morphology and Organization in Cleared Plant Tissues Treated with Cell Cycle Inhibitors.- Isolation of Plant Nuclei at Defined Cell Cycle Stages Using Edu Labeling and Flow Cytometry.- Preparation and Fluorescent Analysis of Plant Metaphase Chromosomes.- Fluorescence In Situ Hybridization On Rice Chromosomes.- Dynamics of the Plant Nuclear Envelope During Cell Division.- Immunolabeling Of Nuclei/Chromosomes In Arabidopsis Thaliana.- In Vivo Imaging Of Microtubules Organization In Dividing Giant Cell.- Real-Time Lineage Analysis to Study Cell Division Orientation in the Arabidopsis Shoot Meristem.- Cell Proliferation Analysis Using Edu Labeling In Whole Plant and Histological Samples Of Arabidopsis.- Studying Cell Division Plane Positioning in Early-Stage Embryos.- Characterization Oof Cytokinetic Mutants using Small Fluorescent Probes.- Using Live Cell Markers in Maize to Analyze Cell Division Orientation and Timing.- Ratiometric Fluorescence Live Imaging Analysis of Membrane Lipid Order in Arabidopsis Mitotic Cells Using a Lipid Order-Sensitive Probe.
Inhaltsverzeichnis
Part I: Cell Cultures for Cell Division Studies
1. Synchronization Of Green Algae By Light And Dark Regimes For Cell Cycle and Cell Division Studies
Monika Hlavová, Milada Vítová, and Kate¿ina Bišová
2. Plant Cell Division Analyzed by Transient Agrobacterium-Mediated Transformation of Tobacco BY-2 Cells
Henrik Buschmann
Part II: Cell Cycle and Mitosis
3. Identifying Gene Regulatory Networks In Arabidopsis By In Silico Prediction, Yeast-1-Hybrid and Inducible Gene Profiling Assays
Erin E. Sparks and Philip N. Benfey
4. Cytrap Marker Systems for In Vivo Visualization of Cell Cycle Progression in Arabidopsis
Shiori Sugamata Aki and Masaaki Umeda
5. Imaging Nuclear Morphology and Organization in Cleared Plant Tissues Treated with Cell Cycle Inhibitors
José Dijair Antonino De Souza Junior, Maria Fatima Grossi De Sa, Gilbert Engler and, Janice De Almeida Engler
6. Isolation of Plant Nuclei at Defined Cell Cycle Stages Using Edu Labeling and Flow Cytometry
Emily E. Wear, Lorenzo Concia, Ashley M. Brooks, Emily A. Markham, Tae-Jin Lee, George C. Allen, William F. Thompson, and Linda Hanley-Bowdoin
7. Preparation and Fluorescent Analysis of Plant Metaphase Chromosomes
Trude Schwarzacher
8. Fluorescence In Situ Hybridization On Rice Chromosomes
Yafei Li and Zhukuan Cheng
Part III Imaging Plant Cell Division
9. Dynamics of the Plant Nuclear Envelope During Cell Division
David E Evans and Katja Graumann
10. Immunolabeling Of Nuclei/Chromosomes In Arabidopsis Thaliana
Michael Sandmann, Joerg Fuchs, and Inna Lermontova
11. In Vivo Imaging Of Microtubules Organization In Dividing Giant Cell
Marie-Cecile Caillaud and Bruno Favery
Part IV: Cell Division and Morphogenesis
12. Real-Time Lineage Analysis to Study Cell Division Orientation in the Arabidopsis Shoot Meristem
Cory J. Tobin and Elliot M. Meyerowitz
13. Cell Proliferation Analysis Using Edu Labeling In Whole Plant and Histological Samples Of Arabidopsis
Anita Kazda, Svetlana Akimcheva, J. Matthew Watson, and Karel Riha
14. Studying Cell Division Plane Positioning in Early-Stage Embryos
Katia Belcram, Jean-Christophe Palauqui, and Martine Pastuglia
Part V: Cytokinesis
15. Characterization Oof Cytokinetic Mutants using Small Fluorescent Probes
Andrei Smertenko, Panagiotis Moschou, Laining Zhang, Deirdre Fahy, and Peter Bozhkov
16. Using Live Cell Markers in Maize to Analyze Cell Division Orientation and Timing Carolyn G. Rasmussen
17. Ratiometric Fluorescence Live Imaging Analysis of Membrane Lipid Order in Arabidopsis Mitotic Cells Using a Lipid Order-Sensitive Probe
Patricia Gerbeau-Pissot, Christophe Der, Markus Grebe, and Thomas Stanislas
Klappentext
This volume aims to present a large panel of techniques for the study of Plant Cell Division. Plant Cell Division: Methods and Protocols captures basic experimental protocols that are commonly used to study plant cell division processes, as well as more innovative procedures. Chapters are split into five parts covering several different aspect of plant cell division such as, cell cultures for cell division studies, cell cycle progression and mitosis, imaging plant cell division, cell division and morphogenesis, and cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Cell Division: Methods and Protocols is a valuable tool for the study of plant cell division at both the cellular and molecular levels, and in the context of plant development.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
