Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
This book encompasses broad aspects of
Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for
in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.
The chapter "Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
1. Cryptosporidium Diagnostic Assays: Microscopy
Guy Robinson and Rachel M. Chalmers
2. Cryptosporidium Diagnostic Assays: Molecular Detection
Guy Robinson, Kristin Elwin, and Rachel M. Chalmers
3. Detection of Cryptosporidium Recovered From Large-Volume Water Samples Using Dead-End Ultrafiltration
Amy M. Kahler and Vincent R. Hill
4. Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation
Michael J. Arrowood
5. Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay
Jeffrey W. Priest and Delynn M. Moss
6. Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii
Iwona Driskell and Roberta M. O´Connor
7. Cryptosporidium Genotyping for Epidemiology Tracking
Dawn M. Roellig and Lihua Xiao
8. Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection
Anson V. Koehler and Jan Šlapeta
9. Preparation of Cryptosporidium DNA for Whole Genome Sequencing
Guy Robinson and Rachel M. Chalmers
10. Accessing Cryptosporidium Omic and Isolate Data Via CryptoDB.org
Susanne Warrenfeltz and Jessica C. Kissinger on behalf of the EuPathDB Team
11. A Novel Method to Silence Genes in Cryptosporidium
Alejandro Castellanos-Gonzalez
12. Use of miRNAs to Study Host Cell-Parasite Interactions
Ji-Hao Xu, Nicholas W. Mathy, and Xian-Ming Chen
13. Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9
Adam Sateriale, Mattie Pawlowic, Sumiti Vinayak, Carrie Brooks, and Boris Striepen
14. Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology
Karine Sonzogni-Desautels, Jan R. Mead, and Momar Ndao
15. Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics
Michael W. Riggs and Deborah A. Schaefer
16. Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water
Jennifer L. Murphy and Michael J. Arrowood
17. High-Content Screening for Cryptosporidium Drug Discovery
Melissa S. Love and Case W. McNamara
18. High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR
Haili Zhang and Guan Zhu
19. In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds
Nigel Yarlett, Mary Morada, Mohini Gobin, Wesley Van Voorhis, and Samuel Arnold
20. In Vitro Culture of Cryptosporidium parvum Using Stem Cell-Derived Intestinal Epithelial Monolayers
Georgia Wilke, Yi Wang, Soumya Ravindran, Thaddeus Stappenbeck, William Witola, and L. David Sibley
21. Two and Three Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium
Daviel Cardenas, Seema Bhalchandra, Hymlaire Lamisere, Ying Chen, Xi-Lei Zeng, Sasirekha Ramani, Umesh C. Karandikar, David L. Kaplan, Mary K. Estes, and Honorine D. Ward
This book encompasses broad aspects of
Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for
in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.
The chapter "Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
This book encompasses broad aspects of
Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for
in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.
The chapter "Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Cryptosporidium Diagnostic Assays: Microscopy.- Cryptosporidium Diagnostic Assays: Molecular Detection.- Detection of Cryptosporidium Recovered From Large-Volume Water Samples Using Dead-End Ultrafiltration.- Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation.- Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay.- Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii.- Cryptosporidium Genotyping for Epidemiology Tracking.- Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection.- Preparation of Cryptosporidium DNA for Whole Genome Sequencing.- Accessing Cryptosporidium Omic and Isolate Data Via CryptoDB.org.- A Novel Method to Silence Genes in Cryptosporidium.- Use of miRNAs to Study Host Cell-Parasite Interactions.- Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9.- Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology.- Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics.- Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water.- High-Content Screening for Cryptosporidium Drug Discovery.- High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR.- In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds.- In Vitro Culture of Cryptosporidium parvum Using Stem Cell-Derived Intestinal Epithelial Monolayers.- Two and Three Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium.
Inhaltsverzeichnis
1. Cryptosporidium Diagnostic Assays: Microscopy
Guy Robinson and Rachel M. Chalmers
2. Cryptosporidium Diagnostic Assays: Molecular Detection
Guy Robinson, Kristin Elwin, and Rachel M. Chalmers
3. Detection of Cryptosporidium Recovered From Large-Volume Water Samples Using Dead-End Ultrafiltration
Amy M. Kahler and Vincent R. Hill
4. Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation
Michael J. Arrowood
5. Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay
Jeffrey W. Priest and Delynn M. Moss
6. Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii
Iwona Driskell and Roberta M. O¿Connor
7. Cryptosporidium Genotyping for Epidemiology Tracking
Dawn M. Roellig and Lihua Xiao
8. Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection
Anson V. Koehler and Jan Šlapeta
9. Preparation of Cryptosporidium DNA for Whole Genome Sequencing
Guy Robinson and Rachel M. Chalmers
10. Accessing Cryptosporidium Omic and Isolate Data Via CryptoDB.org
Susanne Warrenfeltz and Jessica C. Kissinger on behalf of the EuPathDB Team
11. A Novel Method to Silence Genes in Cryptosporidium
Alejandro Castellanos-Gonzalez
12. Use of miRNAs to Study Host Cell-Parasite Interactions
Ji-Hao Xu, Nicholas W. Mathy, and Xian-Ming Chen
13. Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9
Adam Sateriale, Mattie Pawlowic, Sumiti Vinayak, Carrie Brooks, and Boris Striepen
14. Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology
Karine Sonzogni-Desautels, Jan R. Mead, and Momar Ndao
15. Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics
Michael W. Riggs and Deborah A. Schaefer
16. Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water
Jennifer L. Murphy and Michael J. Arrowood
17. High-Content Screening for Cryptosporidium Drug Discovery
Melissa S. Love and Case W. McNamara
18. High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR
Haili Zhang and Guan Zhu
19. In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds
Nigel Yarlett, Mary Morada, Mohini Gobin, Wesley Van Voorhis, and Samuel Arnold
20. In Vitro Culture of Cryptosporidium parvum Using Stem Cell-Derived Intestinal Epithelial Monolayers
Georgia Wilke, Yi Wang, Soumya Ravindran, Thaddeus Stappenbeck, William Witola, and L. David Sibley
21. Two and Three Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium
Daviel Cardenas, Seema Bhalchandra, Hymlaire Lamisere, Ying Chen, Xi-Lei Zeng, Sasirekha Ramani, Umesh C. Karandikar, David L. Kaplan, Mary K. Estes, and Honorine D. Ward
Klappentext
This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
rn
Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.
rn
The chapter "Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.rn
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
