Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Andrew Hill, Associate Professor/Principle Research Fellow, Department of Biochemistry and Molecular Biology, The University of Melbourne Bio21 Institute,
Email: a.hill@unimelb.edu.au
This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and comprehensive, Exosomes and Microvesicles: Methods and Protocols is a valuable resource containing methodologies for anyone interested in researching EVs.
Preface...
Table of Contents...
Contributing Authors...
1. Methods to Analyze EVs
Bernd Giebel and Clemens Helmbrecht
2. Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles
Sybren L. N. Maas, Marike L.D. Broekman, and Jeroen de Vrij
3. Immuno-Characterization of Exosomes using Nanoparticle Tracking Analysis
Kym McNicholas and Michael Z. Michael
4. Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy
Romain Linares, Sisareuth Tan, Céline Gounou, and Alain R. Brisson
5. Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR
Shayne A. Bellingham, Mitch Shambrook, and Andrew F. Hill
6. Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM™ System
Lesley Cheng and Andrew F. Hill
7. A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration
Rong Xu, Richard J. Simpson, and David W. Greening
8. Multiplexed Phenotyping of Extracellular Vesicles using Protein Microarray (EV Array)
Rikke Bæk and Malene Møller Jørgensen
9. Purification and Analysis of Exosomes Released by Mature Cortical Neurons following Synaptic Activation
Karine Laulagnier, Charlotte Javalet, Fiona Hemming, and Rémy Sadoul
10. A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space
R. Perez-Gonzalez, S.A. Gauthier, A. Kumar, M. Saito, M. Saito, and E. Levy
11. Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission
Pascal Leblanc, Zaira E. Arellano-Anaya, Emilien Bernard, Laure Gallay, Monique Provansal, Sylvain Lehmann, Laurent Schaeffer, Graça Raposo and Didier Vilette
12. Isolation of Platelet-Derived Extracellular Vesicles
Maria Aatonen, Sami Valkonen, Anita Böing, Yuana Yuana, Rienk Nieuwland and Pia Siljander
13. Bioinformatics Tools for Extracellular Vesicles Research
Shivakumar Keerthikumar, Lahiru Gangoda, Yong Song Gho, and Suresh Mathivanan
14. Preparation and Isolation of siRNA-Loaded Extracellular Vesicles
Pieter Vader, Imre Mäger, Yi Lee, Joel Z. Nordin, Samir EL Andaloussi, and Matthew J.A. Wood
15. Interaction of Extracellular Vesicles with Endothelial Cells under Physiological Flow Conditions
S.M. van Dommelen, M. Fish, A.D. Barendrecht, R.M. Schiffelers, O. Eniola-Adefeso, P. Vader
This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and comprehensive, Exosomes and Microvesicles: Methods and Protocols is a valuable resource containing methodologies for anyone interested in researching EVs.
This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and comprehensive, Exosomes and Microvesicles: Methods and Protocols is a valuable resource containing methodologies for anyone interested in researching EVs.
Methods to Analyze EVs.- Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.- Immuno-Characterization of Exosomes using Nanoparticle Tracking Analysis.- Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy.- Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR.- Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM(TM) System.- A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration.- Multiplexed Phenotyping of Extracellular Vesicles using Protein Microarray (EV Array).- Purification and Analysis of Exosomes Released by Mature Cortical Neurons following Synaptic Activation.- A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space.- Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission.- Isolation of Platelet-Derived Extracellular Vesicles.- Bioinformatics Tools for Extracellular Vesicles Research.- Preparation and Isolation of siRNA-Loaded Extracellular Vesicles.- Interaction of Extracellular Vesicles with Endothelial Cells under Physiological Flow Conditions.
Über den Autor
Inhaltsverzeichnis
Methods to Analyze EVs.- Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.- Immuno-Characterization of Exosomes using Nanoparticle Tracking Analysis.- Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy.- Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR.- Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM(TM) System.- A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration.- Multiplexed Phenotyping of Extracellular Vesicles using Protein Microarray (EV Array).- Purification and Analysis of Exosomes Released by Mature Cortical Neurons following Synaptic Activation.- A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space.- Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission.- Isolation of Platelet-Derived Extracellular Vesicles.- Bioinformatics Tools for Extracellular Vesicles Research.- Preparation and Isolation of siRNA-Loaded Extracellular Vesicles.- Interaction of Extracellular Vesicles with Endothelial Cells under Physiological Flow Conditions.
Klappentext
This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.nnPractical and comprehensive, Exosomes and Microvesicles: Methods and Protocols is a valuable resource containing methodologies for anyone interested in researching EVs.nn
Includes cutting-edge methods and protocols
n
Provides step-by-step detail essential for reproducible results
n
Contains key notes and implementation advice from the experts
n
Includes supplementary material: sn.pub/extras
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