Includes cutting-edge methods and protocols
Provides step-by-step detail for reproducible results
Contains key notes and implementation advice from the experts
This volume details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Genome Editing in Animals: Methods and Protocols aims to ensure successful results in the further study of this vital field.
1. Construction and Evaluation of Zinc Finger Nucleases
Hiroshi Ochiai and Takashi Yamamoto
2. Current Overview of TALEN Construction Systems
Tetsushi Sakuma and Takashi Yamamoto
3. CRISPR/Cas9
Izuho Hatada and Takuro Horii
4. Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse
Qingbo Wang and Kumiko Ui-Tei
5. Genome Editing of Mouse by Cytoplasmic Injection
Takuro Horii and Izuho Hatada
6. Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing sgRNA/Cas9 Expressing Plasmids
Taichi Noda, Asami Oji, and Masahito Ikawa
7. Genome Editing in Mouse and Rat by Electroporation
Takehito Kaneko
8. Generation of Knock-in Mouse by Genome Editing
Wataru Fujii
9. Genome Editing of Rat
Takehito Kaneko
10. Gene Targeting in Rabbits: Single-step Generation of Knockout Rabbits by Microinjection of CRISPR/Cas9 Plasmids
Yoshihiro Kawano and Arata Honda
11. Genome Editing of Pig
Masahito Watanabe and Hiroshi Nagashima
12. Genome Editing of Monkey
Zhen Liu, Yijun Cai, and Qiang Sun
13. Genome Editing Mediated by Primordial Germ Cell in Chicken
Jae Yong Han and Hong Jo Lee
14. CRISPR/Cas9-mediated Targeted Knock-in of Exogenous Reporter Genes in Zebrafish
Atsuo Kawahara
15. Genome Editing of Medaka
Satoshi Ansai and Masato Kinoshita
16. A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders using the CRISPR-Cas System
Yuto Sakane, Ken-ich T Suzuki, and Takashi Yamamoto
17. Genome Editing of Silkworms
Takuya Tsubota and Hideki Sezutsu
18. Genome Editing in the Cricket, Gryllus bimaculatus
Takahito Watanabe, Sumihare Noji, and Taro Mito
19. Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease
Yasunori Sasakura, Keita Yoshida, and Nicholas Treen
20. Genome editing of C. elegans
Takuma Sugi
This volume details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the highly successful
Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Genome Editing in Animals: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Construction and Evaluation of Zinc Finger Nucleases.- Current Overview of TALEN Construction Systems.- CRISPR/Cas9.- Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse.- Genome Editing of Mouse by Cytoplasmic Injection.- Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing sgRNA/Cas9 Expressing Plasmids.- Genome Editing in Mouse and Rat by Electroporation.- Generation of Knock-in Mouse by Genome Editing.- Genome Editing of Rat.- Gene Targeting in Rabbits: Single-step Generation of Knockout Rabbits by Microinjection of CRISPR/Cas9 Plasmids.- Genome Editing of Pig.- Genome Editing of Monkey.- Genome Editing Mediated by Primordial Germ Cell in Chicken.- CRISPR/Cas9-mediated Targeted Knock-in of Exogenous Reporter Genes in Zebrafish.- Genome Editing of Medaka.- A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders using the CRISPR-Cas System.- Genome Editing of Silkworms.- Genome Editing in the Cricket, Gryllus bimaculatus.- Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease.- Genome editing of C. elegans.
Inhaltsverzeichnis
Construction and Evaluation of Zinc Finger Nucleases.- Current Overview of TALEN Construction Systems.- CRISPR/Cas9.- Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse.- Genome Editing of Mouse by Cytoplasmic Injection.- Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing sgRNA/Cas9 Expressing Plasmids.- Genome Editing in Mouse and Rat by Electroporation.- Generation of Knock-in Mouse by Genome Editing.- Genome Editing of Rat.- Gene Targeting in Rabbits: Single-step Generation of Knockout Rabbits by Microinjection of CRISPR/Cas9 Plasmids.- Genome Editing of Pig.- Genome Editing of Monkey.- Genome Editing Mediated by Primordial Germ Cell in Chicken.- CRISPR/Cas9-mediated Targeted Knock-in of Exogenous Reporter Genes in Zebrafish.- Genome Editing of Medaka.- A Simple Protocol for Loss-of-Function Analysis in
Xenopus tropicalis
Founders using the CRISPR-Cas System.- Genome Editing of Silkworms.- Genome Editing in the Cricket,
Gryllus bimaculatus.-
Genome Editing of the Ascidian
Ciona intestinalis
with TALE Nuclease.- Genome editing of
C. elegans.
Klappentext
This volume details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.nAuthoritative and practical, Genome Editing in Animals: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Includes cutting-edge methods and protocols
Provides step-by-step detail for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Includes supplementary material: sn.pub/extras
