Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.
Part I Review Chapters
1. Advanced in Plant Gene Silencing Methods
Prachi Pandey, Muthappa Senthil-Kumar, and Kirankumar S. Mysore
2. Strategies for Altering Plant Traits Using Virus-Induced Gene Silencing Technologies
Christophe Lacomme
3. Bioinformatics Tools for Achieving Better Gene Silencing in Plants
Firoz Ahmed, Xinbin Dai, and Patrick Xuechun Zhao
4. Profiling of Small RNAs Involved in Plant-Pathogen Interactions
Dongdong Niu, Zhaoyun Wang, Shune Wang, Lulu Qiao, and Hongwei Zhao
5. RNAi-Mediated Resistance to Viruses in Genetically Engineered Plants
Abdulrazak B. Ibrahim and Francisco J. L. Aragão
Part II Methods Chapter
6. Simplifying Transgene Locus Structure Through Cre-lox Recombination
Vibha Srivastava and David W. Ow
7. Transgene-Induced Gene Silencing in Plants
Yun Jin and Hui-Shan Guo
8. Gene Silencing by DNA Interference in Fern Gametophytes
Masamitsu Wada and Hidenori Tsuboi
9. Induction of Stable Epigenetic Gene Silencing in Plants Using a Virus Vector
Akira Kanazawa and Megumi Kasai
10. A Method for Validating MicroRNAs in Plants by miR-RACE
Jinggui Fang and Xin Sun
11. MR VIGS: MicroRNA-Based Virus-Induced Gene Silencing in Plants
Weiwei Chen, Qj Zhang, Junhua Kong, Feng Hu, Bin Li, Chaoqun Wu, Cheng Qin, Pencheng Zhang, Nongnong Shi, and Yiguo Hong
12. A High-Throughput RNA Interference (RNAi)-Based Approach Using Hairy Roots for the Study of Plant-Rhizobia Interactions
Senjuti Sinharoy, Catalina I. Pislariu, and Michael K. Udvardi
13. A Functional Genomics Method for Assaying Gene Function in Phytopathogenic Fungi Through Host-Induced Gene Silencing Mediated by Agroinfiltration
Vinay Panwar, Brent McCallum, and Guus Bakkeren
14. An Effective and Convenient Method for the Delivery of Apple Latent Spherical Virus (ALSV)-Based Vectors into Plant Cells by Agroinoculation
Tatsuya Kon and Nubuyuki Yoshikawa
15. Virus-Induced Gene Silencing (VIGS) for Functional Genomics in Rice Using rice tungro bacilliform virus (RTBV) as a Vector
Ravi Kant, Shweta Sharma, and Indranil Dasgupta
16. Virus-Induced Gene Silencing of Fiber-Related Genes in Cotton
John R. Tuttle, Candace H. Haigler, and Dominique Robertson
17. Establishment of an Efficient Virus-Induced Gene Silencing (VIGS) Assay in Arabidopsis by Agrobacterium-Mediated Rubbing Infection
Ana Marcia E. de A. Manhães, Marcos V. V. de Oliveira, and Libo Shan
18. Virus-Induced Gene Silencing as a Scalable Tool to Study Drought Tolerance in Plants
Gavin M. George, Michael E. Ruckle, and James R. Lloyd
19. VIGS for Dissecting Mechanisms Involved in the Symbiotic Interaction of Microbes with Plants
Mette Grønlund
20. Construction of a Cotton VIGS Library for Functional Genomic Study
Maoying Li, Fangjun Li, and Ping He
21. Synthetic Gene Complementation to Determine Off-Target Silencing
Dhirendra Kumar
22. Construction of Mismatched Inverted Repeat (IR) Silencing Vectors for Maximizing IR Stability and Effective Gene Silencing and in Plants
M. E. Chrissie Rey, Johan Harmse, Sarah H. Taylor, Patrick Arbuthnot, and Marc S. Weinberg
This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.
This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.
Advanced in Plant Gene Silencing Methods.- Strategies for Altering Plant Traits Using Virus-Induced Gene Silencing Technologies.- Bioinformatics Tools for Achieving Better Gene Silencing in Plants.- Profiling of Small RNAs Involved in Plant-Pathogen Interactions.- RNAi-Mediated Resistance to Viruses in Genetically Engineered Plants.- Simplifying Transgene Locus Structure Through Cre-lox Recombination.- Transgene-Induced Gene Silencing in Plants.- Gene Silencing by DNA Interference in Fern Gametophytes.- Induction of Stable Epigenetic Gene Silencing in Plants Using a Virus Vector.- A Method for Validating MicroRNAs in Plants by miR-RACE.- MR VIGS: MicroRNA-Based Virus-Induced Gene Silencing in Plants.- A High-Throughput RNA Interference (RNAi)-Based Approach Using Hairy Roots for the Study of Plant-Rhizobia Interactions.- A Functional Genomics Method for Assaying Gene Function in Phytopathogenic Fungi Through Host-Induced Gene Silencing Mediated by Agroinfiltration.- An Effective and Convenient Method for the Delivery of Apple Latent Spherical Virus (ALSV)-Based Vectors into Plant Cells by Agroinoculation.- Virus-Induced Gene Silencing (VIGS) for Functional Genomics in Rice Using rice tungro bacilliform virus (RTBV) as a Vector.- Virus-Induced Gene Silencing of Fiber-Related Genes in Cotton.- Establishment of an Efficient Virus-Induced Gene Silencing (VIGS) Assay in Arabidopsis by Agrobacterium-Mediated Rubbing Infection.- Virus-Induced Gene Silencing as a Scalable Tool to Study Drought Tolerance in Plants.- VIGS for Dissecting Mechanisms Involved in the Symbiotic Interaction of Microbes with Plants.- Construction of a Cotton VIGS Library for Functional Genomic Study.- Synthetic Gene Complementation to Determine Off-Target Silencing.- Construction of Mismatched Inverted Repeat (IR) Silencing Vectors for Maximizing IR Stability and Effective Gene Silencing and in Plants.
Inhaltsverzeichnis
Advanced in Plant Gene Silencing Methods.- Strategies for Altering Plant Traits Using Virus-Induced Gene Silencing Technologies.- Bioinformatics Tools for Achieving Better Gene Silencing in Plants.- Profiling of Small RNAs Involved in Plant-Pathogen Interactions.- RNAi-Mediated Resistance to Viruses in Genetically Engineered Plants.- Simplifying Transgene Locus Structure Through Cre-lox Recombination.- Transgene-Induced Gene Silencing in Plants.- Gene Silencing by DNA Interference in Fern Gametophytes.- Induction of Stable Epigenetic Gene Silencing in Plants Using a Virus Vector.- A Method for Validating MicroRNAs in Plants by miR-RACE.- MR VIGS: MicroRNA-Based Virus-Induced Gene Silencing in Plants.- A High-Throughput RNA Interference (RNAi)-Based Approach Using Hairy Roots for the Study of Plant-Rhizobia Interactions.- A Functional Genomics Method for Assaying Gene Function in Phytopathogenic Fungi Through Host-Induced Gene Silencing Mediated by Agroinfiltration.- An Effective and Convenient Method for the Delivery of Apple Latent Spherical Virus (ALSV)-Based Vectors into Plant Cells by Agroinoculation.- Virus-Induced Gene Silencing (VIGS) for Functional Genomics in Rice Using rice tungro bacilliform virus (RTBV) as a Vector.- Virus-Induced Gene Silencing of Fiber-Related Genes in Cotton.- Establishment of an Efficient Virus-Induced Gene Silencing (VIGS) Assay in Arabidopsis by Agrobacterium-Mediated Rubbing Infection.- Virus-Induced Gene Silencing as a Scalable Tool to Study Drought Tolerance in Plants.- VIGS for Dissecting Mechanisms Involved in the Symbiotic Interaction of Microbes with Plants.- Construction of a Cotton VIGS Library for Functional Genomic Study.- Synthetic Gene Complementation to Determine Off-Target Silencing.- Construction of Mismatched Inverted Repeat (IR) Silencing Vectors for Maximizing IR Stability and Effective Gene Silencing and in Plants.
Klappentext
This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.nThorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
