Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo tranillegalscriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.
1. Growth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse
Stephany El-Hayek, Qin Yang, and Hugh J. Clarke
2. In vitro Culture of Mouse Oocytes for Meiotic Maturation
Ewa Borsuk and Jacek Z. Kubiak
3. In vitro Maturation and Culture of Human Oocytes
Jennifer R Gruhn, Stine Gry Kristensen, Claus Yding Andersen, and Eva R Hoffmann
4. Pronuclear Transfer in Human Oocytes
Louise Hyslop
5. In vitro Fertilization of Mouse Oocyte
Agathe Chaigne
6. Profiling Maternal mRNA Translation during Oocyte Development
Joao P. Sousa Martins and Marco Conti
7. Tranillegalscriptome Profiling of Single Mouse Oocytes
Maud Borensztein , Laurène Syx
, Nicolas Servant,
and Edith Heard
8. Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation
Cecilia S. Blengini and Karen Schindler
9. Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation
Jessica Greaney, Zhe Wei, and Hayden Homer
10. Triple-colour Live Imaging of Mouse Oocytes
Aurélien Courtois, Petr Solc, and Tomoya S. Kitajima
11. Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes
Elvira Nikalayevich, Nora Bouftas, and Katja Wassmann
12. Manipulating Cohesin Levels in Live Mouse Oocytes
Anna Szydlowska, Sabrina Ladstätter, and Kikuë Tachibana
13. Optogenetic Manipulation of Mouse Oocytes
Takashi Akera, David M. Chenoweth, and Michael A. Lampson
14. Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin
Greg FitzHarris
15. Photoactivation of Actin in Mouse Oocyte
Maria Almonacid
16. Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes
Isma Bennabi and Marion Manil-Ségalen
17. Micropipette Aspiration of Oocytes to Assess Cortical Tension
Janice P. Evans and Douglas N. Robinson
This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo tranillegalscriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.
"Each chapter presents not only detailed and reproducible methods (troubleshooting included) but also nice images and instructive figures providing non-verbal information necessary to better understand how to deal with this `fantastic little laboratory of molecular biology´ that is the oocyte.” (Manuela Monti, European Journal of Histochemistry, Vol. 62 (04), October, 2018)
This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo tranillegalscriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.
Growth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse.- In vitro Culture of Mouse Oocytes for Meiotic Maturation.- In vitro Maturation and Culture of Human Oocytes.- Pronuclear Transfer in Human Oocytes.- In vitro Fertilization of Mouse Oocyte.- Profiling Maternal mRNA Translation during Oocyte Development.- Tranillegalscriptome Profiling of Single Mouse Oocytes.- Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation.- Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation.- Triple-colour Live Imaging of Mouse Oocytes.- Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes.- Manipulating Cohesin Levels in Live Mouse Oocytes.- Optogenetic Manipulation of Mouse Oocytes.- Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin.- Photoactivation of Actin in Mouse Oocyte.- Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes.- Micropipette Aspiration of Oocytes to Assess Cortical Tension.
"Each chapter presents not only detailed and reproducible methods (troubleshooting included) but also nice images and instructive figures providing non-verbal information necessary to better understand how to deal with this 'fantastic little laboratory of molecular biology' that is the oocyte." (Manuela Monti, European Journal of Histochemistry, Vol. 62 (04), October, 2018)
Inhaltsverzeichnis
1. Growth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse
Stephany El-Hayek, Qin Yang, and Hugh J. Clarke
2. In vitro Culture of Mouse Oocytes for Meiotic Maturation
Ewa Borsuk and Jacek Z. Kubiak
3. In vitro Maturation and Culture of Human Oocytes
Jennifer R Gruhn, Stine Gry Kristensen, Claus Yding Andersen, and Eva R Hoffmann
4. Pronuclear Transfer in Human Oocytes
Louise Hyslop
5. In vitro Fertilization of Mouse Oocyte
Agathe Chaigne
6. Profiling Maternal mRNA Translation during Oocyte Development
Joao P. Sousa Martins and Marco Conti
7. Tranillegalscriptome Profiling of Single Mouse Oocytes
Maud Borensztein , Laurène Syx
, Nicolas Servant,
and Edith Heard
8. Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation
Cecilia S. Blengini and Karen Schindler
9. Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation
Jessica Greaney, Zhe Wei, and Hayden Homer
10. Triple-colour Live Imaging of Mouse Oocytes
Aurélien Courtois, Petr Solc, and Tomoya S. Kitajima
11. Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes
Elvira Nikalayevich, Nora Bouftas, and Katja Wassmann
12. Manipulating Cohesin Levels in Live Mouse Oocytes
Anna Szydlowska, Sabrina Ladstätter, and Kikuë Tachibana
13. Optogenetic Manipulation of Mouse Oocytes
Takashi Akera, David M. Chenoweth, and Michael A. Lampson
14. Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin
Greg FitzHarris
15. Photoactivation of Actin in Mouse Oocyte
Maria Almonacid
16. Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes
Isma Bennabi and Marion Manil-Ségalen
17. Micropipette Aspiration of Oocytes to Assess Cortical Tension
Janice P. Evans and Douglas N. Robinson
Klappentext
This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo tranillegalscriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.nnn nnnAuthoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
