Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
This detailed volume focuses on best practices and conditions for maintaining the most commonly used salamander species in the laboratory. Salamanders in Regeneration Research: Methods and Protocols guides readers through experimental manipulations in vivo and in vitro, respectively. With methods on targeting a wide variety of structures, ranging from the limb to the heart and to the brain, and methods for studying genetically modified organisms and tools for mining in the genomic databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Salamanders in Regeneration Research: Methods and Protocols provides a comprehensive collection of methods chapters.
Part I: Salamanders
1. Variation in Salamanders: An Essay on Genomes, Development, and Evolution
Jeremy P Brockes
2. Maintaining Eastern Newts (Notophthalmus viridescens) for Regeneration Research
Hans-Georg Simon and Shannon Odelberg
3. Housing and Maintenance of Ambystoma mexicanum, the Mexican Axolotl
Johanna E. Farkas and James R. Monaghan
4. Husbandry of Spanish Ribbed Newts (Pleurodeles waltl)
Alberto Joven, Matthew Kirkham, and András Simon
5. Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies
Claudia Marcela Arenas G, Andrea Gómez-Molina, and Jean Paul Delgado
Part II: Experimental Manipulation in Salamanders
6. Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISH
Nobuyasu. Maki
7. Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablation
Matthew Kirkham and Alberto Joven
8. The Accessory Limb Model: An Alternative Experimental System of Limb Regeneration
Tetsuya Endo, David M. Gardiner, Aki Makanae, and Akira Satoh
9. High-efficiency Electroporation of the Spinal Cord in Larval Axolotl
Aida Rodrigo-Albors and Elly M. Tanaka
10. Pseudotyped Retroviruses for Infecting Axolotl
Tzu-Hsing Kuo and Jessica L. Whited
11. Thyroxine Induced Metamorphosis in the Axolotl (Ambystoma mexicanum)
Peggy S. Coots and Ashley W. Seifert
12. Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryos
Anoop Kumar and Jean Paul Delgado
13. In vivo Modulation and Quantification of microRNAs During Axolotl Tail Regeneration
Jami R. Erickson and Karen Echeverri
Part III: Salamander Cells in Culture
14. Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemas
Patrizia Ferretti and Anoop Kumar
15. Culture and Transfection of Axolotl Cells
Jean-François Denis, Fadi Sader, Patrizia Ferretti, and Stéphane Roy
16. Isolation and Culture of Neurospheres from the Adult Newt Brain
L Shahul Hameed, András Simon
17. Methods for Axolotl Blood Collection, Intravenous Injection and Efficient Leukocyte Isolation from Peripheral Blood and the Regenerating Limb
Ryan J. Debuque and James W. Godwin
18. Assessing Cardiomyocyte Proliferative Capacity in the Newt Heart and Primary Culture
Hans-Georg Simon and Shannon Odelberg
19. Long Term Organ Cultures of Newt Hearts
Tanja Piatkowski and Thomas Braun
20. In vitro Preparation of Newt Inner Ear Sensory Epithelia as a Model for Repair and Regeneration
Ruth R Taylor
Part IV Transgenesis in Salamanders
21. Transgenesis in Axolotl (Ambystoma mexicanum)
Shahryar Khattak and Elly M Tanaka
22. Generating and Identifying Axolotls with Targeted Mutations using Cas9 RNA-guided Nuclease
G Parker Flowers and Craig M Crews
23. Gene Manipulation for Regenerative Studies using the Iberian Ribbed Newt, Pleurodeles waltl
Toshinori Hayashi and Takashi Takeuchi
Part V: Gene Expression
24. Tranillegalscriptomics Using Axolotls
S. Randal Voss, Anthony Athippozhy, and M. Ryan Woodcock
25. Sal-Site: Research Resources for The Mexican Axolotl
Nour W Al Haj Baddar, M Ryan Woodcock, Shivam Khatri, D. Kevin Kump, and S. Randal Voss
26. Data Mining in Newt-omics, the Repository for Omics Data from the Newt
Mario Looso and Thomas Braun
This detailed volume focuses on best practices and conditions for maintaining the most commonly used salamander species in the laboratory. Salamanders in Regeneration Research: Methods and Protocols guides readers through experimental manipulations in vivo and in vitro, respectively. With methods on targeting a wide variety of structures, ranging from the limb to the heart and to the brain, and methods for studying genetically modified organisms and tools for mining in the genomic databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Salamanders in Regeneration Research: Methods and Protocols provides a comprehensive collection of methods chapters.
This detailed volume focuses on best practices and conditions for maintaining the most commonly used salamander species in the laboratory. Salamanders in Regeneration Research: Methods and Protocols guides readers through experimental manipulations in vivo and in vitro, respectively. With methods on targeting a wide variety of structures, ranging from the limb to the heart and to the brain, and methods for studying genetically modified organisms and tools for mining in the genomic databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Salamanders in Regeneration Research: Methods and Protocols provides a comprehensive collection of methods chapters.
Variation in Salamanders: An Essay on Genomes, Development, and Evolution.- Maintaining Eastern Newts (Notophthalmus viridescens) for Regeneration Research.- Housing and Maintenance of Ambystoma mexicanum, the Mexican Axolotl.- Husbandry of Spanish Ribbed Newts (Pleurodeles waltl.- Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies.- Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISH.- Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablation.- The Accessory Limb Model: An Alternative Experimental System of Limb Regeneration.- High-efficiency Electroporation of the Spinal Cord in Larval Axolotl.- Pseudotyped Retroviruses for Infecting Axolotl.- Thyroxine Induced Metamorphosis in the Axolotl (Ambystoma mexicanum).- Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryos.- In vivo Modulation and Quantification of microRNAs During Axolotl Tail Regeneration.- Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemas.- Culture and Transfection of Axolotl Cells.- Isolation and Culture of Neurospheres from the Adult Newt Brain.- Methods for Axolotl Blood Collection, Intravenous Injection and Efficient Leukocyte Isolation from Peripheral Blood and the Regenerating Limb.- Assessing Cardiomyocyte Proliferative Capacity in the Newt Heart and Primary Culture.- Long Term Organ Cultures of Newt Hearts.- In vitro Preparation of Newt Inner Ear Sensory Epithelia as a Model for Repair and Regeneration .- Transgenesis in Axolotl (Ambystoma mexicanum).- Generating and Identifying Axolotls with Targeted Mutations using Cas9 RNA-guided Nuclease.- Gene Manipulation for Regenerative Studies using the Iberian Ribbed Newt, Pleurodeles waltl.- Tranillegalscriptomics Using Axolotls.- Sal-Site: Research Resources for The Mexican Axolotl.- Data Mining in Newt-omics, the Repository for Omics Data from the Newt.
Inhaltsverzeichnis
Part I: Salamanders
1. Variation in Salamanders: An Essay on Genomes, Development, and Evolution
Jeremy P Brockes
2. Maintaining Eastern Newts (Notophthalmus viridescens) for Regeneration Research
Hans-Georg Simon and Shannon Odelberg
3. Housing and Maintenance of Ambystoma mexicanum, the Mexican Axolotl
Johanna E. Farkas and James R. Monaghan
4. Husbandry of Spanish Ribbed Newts (Pleurodeles waltl)
Alberto Joven, Matthew Kirkham, and András Simon
5. Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies
Claudia Marcela Arenas G, Andrea Gómez-Molina, and Jean Paul Delgado
Part II: Experimental Manipulation in Salamanders
6. Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISH
Nobuyasu. Maki
7. Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablation
Matthew Kirkham and Alberto Joven
8. The Accessory Limb Model: An Alternative Experimental System of Limb Regeneration
Tetsuya Endo, David M. Gardiner, Aki Makanae, and Akira Satoh
9. High-efficiency Electroporation of the Spinal Cord in Larval Axolotl
Aida Rodrigo-Albors and Elly M. Tanaka
10. Pseudotyped Retroviruses for Infecting Axolotl
Tzu-Hsing Kuo and Jessica L. Whited
11. Thyroxine Induced Metamorphosis in the Axolotl (Ambystoma mexicanum)
Peggy S. Coots and Ashley W. Seifert
12. Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryos
Anoop Kumar and Jean Paul Delgado
13. In vivo Modulation and Quantification of microRNAs During Axolotl Tail Regeneration
Jami R. Erickson and Karen Echeverri
Part III: Salamander Cells in Culture
14. Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemas
Patrizia Ferretti and Anoop Kumar
15. Culture and Transfection of Axolotl Cells
Jean-François Denis, Fadi Sader, Patrizia Ferretti, and Stéphane Roy
16. Isolation and Culture of Neurospheres from the Adult Newt Brain
L Shahul Hameed, András Simon
17. Methods for Axolotl Blood Collection, Intravenous Injection and Efficient Leukocyte Isolation from Peripheral Blood and the Regenerating Limb
Ryan J. Debuque and James W. Godwin
18. Assessing Cardiomyocyte Proliferative Capacity in the Newt Heart and Primary Culture
Hans-Georg Simon and Shannon Odelberg
19. Long Term Organ Cultures of Newt Hearts
Tanja Piatkowski and Thomas Braun
20. In vitro Preparation of Newt Inner Ear Sensory Epithelia as a Model for Repair and Regeneration
Ruth R Taylor
Part IV Transgenesis in Salamanders
21. Transgenesis in Axolotl (Ambystoma mexicanum)
Shahryar Khattak and Elly M Tanaka
22. Generating and Identifying Axolotls with Targeted Mutations using Cas9 RNA¿guided Nuclease
G Parker Flowers and Craig M Crews
23. Gene Manipulation for Regenerative Studies using the Iberian Ribbed Newt, Pleurodeles waltl
Toshinori Hayashi and Takashi Takeuchi
Part V: Gene Expression
24. Tranillegalscriptomics Using Axolotls
S. Randal Voss, Anthony Athippozhy, and M. Ryan Woodcock
25. Sal-Site: Research Resources for The Mexican Axolotl
Nour W Al Haj Baddar, M Ryan Woodcock, Shivam Khatri, D. Kevin Kump, and S. Randal Voss
26. Data Mining in Newt-omics, the Repository for Omics Data from the Newt
Mario Looso and Thomas Braun
Klappentext
This detailed volume focuses on best practices and conditions for maintaining the most commonly used salamander species in the laboratory. Salamanders in Regeneration Research: Methods and Protocols guides readers through experimental manipulations in vivo and in vitro, respectively. With methods on targeting a wide variety of structures, ranging from the limb to the heart and to the brain, and methods for studying genetically modified organisms and tools for mining in the genomic databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Salamanders in Regeneration Research: Methods and Protocols provides a comprehensive collection of methods chapters.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
