Includes cutting-edge methods and protocols involving laser capture microdissection
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
1. Laser Capture Microdissection: Insights into Methods and Applications
Meera Mahalingam
2. Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues
Rémi Longuespée, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso, and Edwin De Pauw
3. Isolation of DNA and RNA from Laser Microdissected Tissue Samples
Yelena G. Golubeva and Andrew C. Warner
4. Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE
DaRue A. Prieto, Gordon Whitely, Donald J. Johann, Jr., and Josip Blonder
5. Next Generation Sequencing Analysis of Laser-Microdissected Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens
Lavinia Mägel, Stephan Bartels, and Ulrich Lehmann
6. Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model
Nadia P. Castro and Yelena G. Golubeva
7. Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease
Giuseppe Iacomino, Vera Rotondi Aufiero, Pasquale Marena, Antonella Venezia, Riccardo Troncone, Salvatore Auricchio, and Giuseppe Mazzarella
8. Laser Capture Microdissection and Isolation of High Quality RNA from Frozen Endometrial Tissue
Michele Cummings, Georgia Mappa, and Nicolas M. Orsi
9. Laser Microdissection for Human Papillomavirus (HPV) Genotyping Attribution and Methylation Pattern Analyses of Squamous Intraepithelial Lesions
Monica Molano, Suzanne M. Garland, and Alyssa M. Cornall
10. Laser Capture Microdissection and Tranillegalscriptional Analysis of Sub-Populations of the Osteoblast Lineage from Undecalcified Bone
Efrain Pacheco, Rong Hu, and Scott Taylor
11. Cell Type-Specific Laser Capture Microdissection for Gene Expression Profiling in the Human Brain
Sarah A. Mauney, Tsung-Ung W. Woo, and Kai C. Sonntag
12. The Isolation of Pure Populations of Neurons by Laser Capture Microdissection: Methods and Application in Neuroscience
Renée Morris and Prachi Mehta
13. Laser Capture Microdissection in Traumatic Brain Injury Research: Obtaining Hippocampal Subregions and Pools of Injured Neurons for Genomic Analyses
Deborah R. Boone, Harris A. Weisz, Stacy L. Sell, and Helen L. Hellmich
14. Isolation of Distinct Types of Neurons from Fresh Brain Tissue Using Laser Microdissection in Combination with High Performance Liquid Chromatography – Mass Spectrometry
Luisa Aring, Simone Steinbach, Katrin Marcus, and Caroline May
15. Immuno-Guided Laser Capture Microdissection of Glial Cells for mRNA Analysis
Arnaud B. Nicot, Justine Rambeau, Flora Guillot, Alexandra Garcia, and David A. Laplaud
16. Immuno-Laser Capture Microdissection for the Isolation of Enriched Glial Populations from Frozen Post Mortem Human Brain
Julie E. Simpson, Stephen B. Wharton, and Paul R. Heath
17. Laser Capture Microdissection for the Analysis of Rat and Human Spinal Cord Ependyma by qPCR
Daniel Garcia-Ovejero, Beatriz Paniagua-Torija, Angel Arevalo-Martin, Beatriz Navarro-Galve, and Eduardo Molina-Holgado
18. Isolation of Amyloid Plaques and Neurofibrillary Tangles from Archived Alzheimer´s Disease Tissue Using Laser Capture Microdissection for Downstream Proteomics
Eleanor Drummond, Shruti Nayak, Geoffrey Pires, Beatrix Ueberheide, and Thomas Wisniewski
19. Cell-Specific RNA Quantification in Human SN DA Neurons from Heterogeneous Post Mortem Midbrain Samples by UV-Laser Microdissection and RT-qPCR
Johanna Duda, Michael Fauler, Jan Gründemann, and Birgit Liss
20. Laser Capture Microdissection for Layer-Specific Analysis of Enteric Ganglia
Corinna Rosenbaum, Martina Böttner, Thilo Wedel, and Marco Metzger
21. A Laser Microdissection - Liquid Chromatography - Tandem Mass Spectrometry Workflow for Post-Mortem Analysis of Brain Tissue
David C. Hondius, Jeroen J.M. Hoozemans, Annemieke J.M. Rozemuller, Ka Wan Li, and August B. Smit
22. Laser Capture Microdissection and RNA Extraction from Perfusion-Fixed Cartilage and Bone Tissue from Mice Implanted with Human iPSC-Derived MSCs in a Calvarial Defect Model
Xiaonan Xin, Xi Jiang, Alexander Lichtler, Mark Kronenberg, David Rowe, and Joel S. Pachter
23. Laser Capture Microdissection-Based RNA-Seq of Barley Grain Tissues
Ronny Brandt, Martin Mascher, and Johannes Thiel
This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies. The contents of the volume explore these techniques that have revolutionized carrying out molecular analysis on specific types of normal and diseased cells and fully utilizing the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Laser Capture Microdissection: Methods and Protocols, Third Edition seeks to aid researchers generally and pathologists in particular in moving their studies forward with these vital techniques.
This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies. The contents of the volume explore these techniques that have revolutionized carrying out molecular analysis on specific types of normal and diseased cells and fully utilizing the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive,
Laser Capture Microdissection: Methods and Protocols, Third Edition seeks to aid researchers generally and pathologists in particular in moving their studies forward with these vital techniques.
Laser Capture Microdissection: Insights into Methods and Applications.- Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues.- Isolation of DNA and RNA from Laser Microdissected Tissue Samples.- Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE.- Next Generation Sequencing Analysis of Laser-Microdissected Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens.- Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model.- Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease.- Laser Capture Microdissection and Isolation of High Quality RNA from Frozen Endometrial Tissue.- Laser Microdissection for Human Papillomavirus (HPV) Genotyping Attribution and Methylation Pattern Analyses of Squamous Intraepithelial Lesions.- Laser Capture Microdissection and Tranillegalscriptional Analysis of Sub-Populations of the Osteoblast Lineage from Undecalcified Bone.- Cell Type-Specific Laser Capture Microdissection for Gene Expression Profiling in the Human Brain.- The Isolation of Pure Populations of Neurons by Laser Capture Microdissection: Methods and Application in Neuroscience.- Laser Capture Microdissection in Traumatic Brain Injury Research: Obtaining Hippocampal Subregions and Pools of Injured Neurons for Genomic Analyses.- Isolation of Distinct Types of Neurons from Fresh Brain Tissue Using Laser Microdissection in Combination with High Performance Liquid Chromatography - Mass Spectrometry.- Immuno-Guided Laser Capture Microdissection of Glial Cells for mRNA Analysis.- Immuno-Laser Capture Microdissection for the Isolation of Enriched Glial Populations from Frozen Post Mortem Human Brain.- Laser Capture Microdissection for the Analysis of Rat and Human Spinal Cord Ependyma by qPCR.- Isolation of Amyloid Plaques and Neurofibrillary Tangles from Archived Alzheimer's Disease Tissue Using Laser Capture Microdissection for Downstream Proteomics.- Cell-Specific RNA Quantification in Human SN DA Neurons from Heterogeneous Post Mortem Midbrain Samples by UV-Laser Microdissection and RT-qPCR.- Laser Capture Microdissection for Layer-Specific Analysis of Enteric Ganglia.- A Laser Microdissection - Liquid Chromatography - Tandem Mass Spectrometry Workflow for Post-Mortem Analysis of Brain Tissue.- Laser Capture Microdissection and RNA Extraction from Perfusion-Fixed Cartilage and Bone Tissue from Mice Implanted with Human iPSC-Derived MSCs in a Calvarial Defect Model.- Laser Capture Microdissection-Based RNA-Seq of Barley Grain Tissues.
Inhaltsverzeichnis
1. Laser Capture Microdissection: Insights into Methods and Applications
Meera Mahalingam
2. Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues
Rémi Longuespée, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso, and Edwin De Pauw
3. Isolation of DNA and RNA from Laser Microdissected Tissue Samples
Yelena G. Golubeva and Andrew C. Warner
4. Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE
DaRue A. Prieto, Gordon Whitely, Donald J. Johann, Jr., and Josip Blonder
5. Next Generation Sequencing Analysis of Laser-Microdissected Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens
Lavinia Mägel, Stephan Bartels, and Ulrich Lehmann
6. Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model
Nadia P. Castro and Yelena G. Golubeva
7. Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease
Giuseppe Iacomino, Vera Rotondi Aufiero, Pasquale Marena, Antonella Venezia, Riccardo Troncone, Salvatore Auricchio, and Giuseppe Mazzarella
8. Laser Capture Microdissection and Isolation of High Quality RNA from Frozen Endometrial Tissue
Michele Cummings, Georgia Mappa, and Nicolas M. Orsi
9. Laser Microdissection for Human Papillomavirus (HPV) Genotyping Attribution and Methylation Pattern Analyses of Squamous Intraepithelial Lesions
Monica Molano, Suzanne M. Garland, and Alyssa M. Cornall
10. Laser Capture Microdissection and Tranillegalscriptional Analysis of Sub-Populations of the Osteoblast Lineage from Undecalcified Bone
Efrain Pacheco, Rong Hu, and Scott Taylor
11. Cell Type-Specific Laser Capture Microdissection for Gene Expression Profiling in the Human Brain
Sarah A. Mauney, Tsung-Ung W. Woo, and Kai C. Sonntag
12. The Isolation of Pure Populations of Neurons by Laser Capture Microdissection: Methods and Application in Neuroscience
Renée Morris and Prachi Mehta
13. Laser Capture Microdissection in Traumatic Brain Injury Research: Obtaining Hippocampal Subregions and Pools of Injured Neurons for Genomic Analyses
Deborah R. Boone, Harris A. Weisz, Stacy L. Sell, and Helen L. Hellmich
14. Isolation of Distinct Types of Neurons from Fresh Brain Tissue Using Laser Microdissection in Combination with High Performance Liquid Chromatography ¿ Mass Spectrometry
Luisa Aring, Simone Steinbach, Katrin Marcus, and Caroline May
15. Immuno-Guided Laser Capture Microdissection of Glial Cells for mRNA Analysis
Arnaud B. Nicot, Justine Rambeau, Flora Guillot, Alexandra Garcia, and David A. Laplaud
16. Immuno-Laser Capture Microdissection for the Isolation of Enriched Glial Populations from Frozen Post Mortem Human Brain
Julie E. Simpson, Stephen B. Wharton, and Paul R. Heath
17. Laser Capture Microdissection for the Analysis of Rat and Human Spinal Cord Ependyma by qPCR
Daniel Garcia-Ovejero, Beatriz Paniagua-Torija, Angel Arevalo-Martin, Beatriz Navarro-Galve, and Eduardo Molina-Holgado
18. Isolation of Amyloid Plaques and Neurofibrillary Tangles from Archived Alzheimer¿s Disease Tissue Using Laser Capture Microdissection for Downstream Proteomics
Eleanor Drummond, Shruti Nayak, Geoffrey Pires, Beatrix Ueberheide, and Thomas Wisniewski
19. Cell-Specific RNA Quantification in Human SN DA Neurons from Heterogeneous Post Mortem Midbrain Samples by UV-Laser Microdissection and RT-qPCR
Johanna Duda, Michael Fauler, Jan Gründemann, and Birgit Liss
20. Laser Capture Microdissection for Layer-Specific Analysis of Enteric Ganglia
Corinna Rosenbaum, Martina Böttner, Thilo Wedel, and Marco Metzger
21. A Laser Microdissection - Liquid Chromatography - Tandem Mass Spectrometry Workflow for Post-Mortem Analysis of Brain Tissue
David C. Hondius, Jeroen J.M. Hoozemans, Annemieke J.M. Rozemuller, Ka Wan Li, and August B. Smit
22. Laser Capture Microdissection and RNA Extraction from Perfusion-Fixed Cartilage and Bone Tissue from Mice Implanted with Human iPSC-Derived MSCs in a Calvarial Defect Model
Xiaonan Xin, Xi Jiang, Alexander Lichtler, Mark Kronenberg, David Rowe, and Joel S. Pachter
23. Laser Capture Microdissection-Based RNA-Seq of Barley Grain Tissues
Ronny Brandt, Martin Mascher, and Johannes Thiel
Klappentext
This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies. The contents of the volume explore these techniques that have revolutionized carrying out molecular analysis on specific types of normal and diseased cells and fully utilizing the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
n
Authoritative and comprehensive, Laser Capture Microdissection: Methods and Protocols, Third Edition seeks to aid researchers generally and pathologists in particular in moving their studies forward with these vital techniques.
Includes cutting-edge methods and protocols involving laser capture microdissection
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
